An Electrospun Sandwich-Type Lipase-Membrane Bioreactor for Hydrolysis at Macroscopic Oil–Water Interfaces

Abstract

The core task for lipase catalytic system design is to construct a suitable oil−water interface for lipase distribution. In comparison to the micro-oil−water interface, the macro-oil−water interface (top oil−bottom water) served as a simplified lipase catalytic system that is more in line with industrial applications but limited in catalytic efficiency. Based on the assumption that one potential carrier can help lipase reach to the macro-oil−water interface, in the current work, sandwich-type lipase-membrane bioreactors (SLMBs) fabricated by a facile layer-by-layer electrospinning process were reported. These SLMBs were composed of a hydrophilic polyamide 6 nanofibrous membrane (NFM) as the bottom layer, a blended electrospun lipase/PVA NFM as the middle layer, and a hydrophobic EC/PU NFM as the top layer. The lipase loading can be controlled by altering the electrospinning time of the middle layer. Under the optimized conditions, the catalytic efficiency of the SLMBs was 2.05 times higher than that of free lipase. In addition, the SLMBs exhibit much better pH (high activity over a broad pH range of 5−10), temperature (retained 62% at 80 °C), storage stability (no loss of activity after being stored at 4 °C for 11 days), and reusability (retained 23% after five cycles) than free lipase.

Research Progress of Water Treatment Technology Based on Nanofiber Membranes

“…In addition, fresh water suitable for drinking or irrigation can be obtained through the integrated process of combining MBR with the NF/RO system [ 123 ]. In recent years, the application of nanofiber membranes in MBR for the treatment of organic contaminants in water has been widely conducted [ 124 , 125 , 126 , 127 ].…”

Artificial Intelligence Aided Lipase Production and Engineering for Enzymatic Performance Improvement

“…For enzyme preparations, it is not only the immobilization conditions that need to be considered; the selection of immobilization material, activation condition, and reactor are also important factors, − which should have different degrees of influence on the properties of immobilized lipase. Therefore, while AI technology is utilized to computationally optimize immobilization conditions, it can also facilitate the rational design of immobilized enzymes with improved performance, accelerating the discovery of more efficient and sustainable catalysts for various biotechnological applications.…”

The Mesoporous Polydopamine-Based Bowl-like Lipase Nanoreactor for the Biocatalytic Synthesis of Oleate Sterol Ester

“…Mesoporous nanomaterials have been shown to be an excellent alternative for immobilized enzyme carriers in the majority of immobilized carrier materials because of its large specific surface area and adequate pore size, − for providing suitable sites for enzyme attachment. Various of mesoporous materials are emerging in a variety of applications, including sphere-like, − bowl-like, , cube-like, , sandwich-like, , dumbbell-like , and so on. In our opinion, bowl-shaped mesoporous materials would work well as carriers for enzymes to achieve improved catalytic activity.…”