An Electroporation Device with Microbead-Enhanced Electric Field for Bacterial Inactivation

Pudasaini, Sanam; Perera, A T K; Ahmed, Syed Shaheer Uddin; Chong, Yong Bing; Ng, Sum Huan; Yang, Chun

doi:10.3390/inventions5010002

Cited by 16 publications

(14 citation statements)

References 53 publications

(62 reference statements)

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“…In addition to mammalian cells, bacteria have been also reprogrammed in a high‐throughput and efficient manner by using microfluidic electroporation. [ 40,135–138 ] For example, Garcia et al. developed a microfluidic electroporation platform with nonuniform microchannels, which could improve the efficiency and throughput of bacterial electrotransformation ( Escherichia coli ) by four times and 100–1000 times, respectively, compared to that of traditional cuvette electroporation.…”

Section: Programming Living Cellsmentioning

confidence: 99%

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

2021

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Cell‐based living materials, including single cells, cell‐laden fibers, cell sheets, organoids, and organs, have attracted intensive interests owing to their widespread applications in cancer therapy, regenerative medicine, drug development, and so on. Significant progress in materials, microfabrication, and cell biology have promoted the development of numerous promising microfluidic platforms for programming these cell‐based living materials with a high‐throughput, scalable, and efficient manner. In this review, the recent progress of novel microfluidic platforms for programming cell‐based living materials is presented. First, the unique features, categories, and materials and related fabrication methods of microfluidic platforms are briefly introduced. From the viewpoint of the design principles of the microfluidic platforms, the recent significant advances of programming single cells, cell‐laden fibers, cell sheets, organoids, and organs in turns are then highlighted. Last, by providing personal perspectives on challenges and future trends, this review aims to motivate researchers from the fields of materials and engineering to work together with biologists and physicians to promote the development of cell‐based living materials for human healthcare‐related applications.

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Section: Programming Living Cellsmentioning

confidence: 99%

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

2021

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“…In our previous work, we developed a new microfluidic electroporation device with insulating micropillars for inducing locally enhanced electric field to inactivate yeast cells by using a DC field, where pillar diameter to gap ratio (i.e., d/D ratio) was kept as 2 [20]. A scaled-up electroporation device using insulating microbeads to intensify local electric field was also demonstrated [21].…”

Section: Introductionmentioning

confidence: 99%

Bacterial inactivation via microfluidic electroporation device with insulating micropillars

Pudasaini

Perera

et al. 2021

Electrophoresis

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Electroporation is a promising method to inactivate cells and it has wide applications in medical science, biology and environmental health. Here, we investigate the bacteria inactivation performance of two different microfluidic electroporation devices with rhombus and circular micropillars used for generating locally enhanced electric field strength. Experiments are carried out to characterize the inactivation performance (i.e., the log removal efficiency) of two types of bacteria: Escherichia coli (E. coli, gram-negative) and Enterococcus faecalis (E. faecalis, gram-positive) in these two microfluidic devices. We find that under the same applied electric field, the device with rhombus micropillars performs better than the device with circular micropillars for both E. coli and E. faecalis. Numerical simulations show that due to the corner-induced singularity effect, the maximum electric field enhancement is higher in the device with rhombus micropillars than that in the device with circular micropillars. We also study the effects of DC and AC electric fields and flowrate. Our experiments demonstrate that the use of the DC field achieves higher log removal efficiencies than the use of AC field.

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“…A cell electroporation is a modern non-thermal method, which is used in medicine, biotechnology, and industry [1][2][3][4]. It is based on a use of HV pulses, which are applied to tissue by electrodes.…”

Section: Introductionmentioning

confidence: 99%

Compact High-Voltage AC Generator with Pulse Transformer for High-Frequency Irreversible Electroporation (H-FIRE)

Folprecht¹,

Cervinka²,

Procházka³

2021

Electronics

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This paper is focused on a design of a high-voltage (HV) generator, which is proposed for a high-frequency irreversible electroporation (H-FIRE). The generator produces bursts of bipolar symmetrical pulses. Most HV sources used for cell electroporation are based on a controlled discharge of a capacitor into a resistive load. This solution is very simple, but it is associated with a certain risk of an uncontrolled discharge of the capacitor. We present a different type of the generator, where a DC-AC inverter with pulse transformer is used and where the mentioned risk is eliminated. Our generator is able to deliver bursts with variable length from 50 to 150 μs and a gap between bursts can be set from 0.5 to 1.5 s. Pulse frequency can be varied from 65 to 470 kHz and the output voltage is controlled in two ranges from 0 to 1.3 kV or from 0 to 2.5 kV. Results are presented with resistive load and with tissue impedance load.

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An Electroporation Device with Microbead-Enhanced Electric Field for Bacterial Inactivation

Cited by 16 publications

References 53 publications

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

Bacterial inactivation via microfluidic electroporation device with insulating micropillars

Compact High-Voltage AC Generator with Pulse Transformer for High-Frequency Irreversible Electroporation (H-FIRE)

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