An electrophoretic study of maternal, fetal, and infant sera

Moore, Dan H.; Pan, R. Martin Du; Buxton, C. Lee

doi:10.1016/0002-9378(49)90431-7

Cited by 113 publications

(26 citation statements)

References 24 publications

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“…In general, trends for albumin and gamma globulin were downward and for alpha,, alpha2, beta, and phi (fibrinogen) globulins were upward until delivery or later. Subsequently, the trends were [6][7][8][9][10][11][12] reversed and values approached those of the non- Fifth day tistically significant on the basis of plasma concentration, but per 100 gm. of total protein only the decrease in gamma globulin was significant.…”

Section: And Discussionmentioning

confidence: 99%

“…On the tracings, the areas attributable to the various components were defined by the method of Tiselius and Kabat (16), in which ordinates are drawn from the lowest point between each two components to the base line. Each area was measured with a precision disc planimeter 6 or a differential analyzer (17) and the relative concentrations were determined by dividing the areas representative of each component by the area of the entire pattern minus the area of the corresponding boundary anomaly. From this percentage figure and the chemically-determined value for the total plasma protein, corrected for dilution and shrinkage, the actual plasma concentrations of the respective components were calculated.…”

Section: Electrophoretic Techniquementioning

confidence: 99%

“…Although the manufacturer's specifications indicated that the center section of the cell had a capacity of 11 ml., the actual size was 9 + ml. 6 G. Gorado, Zurich, Switzerland.…”

Section: Electrophoretic Techniquementioning

confidence: 99%

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Metabolism of Women During the Reproductive Cycle. Xvii. Changes in Electrophoretic Patterns of Plasma Proteins Throughout the Cycle and Following Delivery 12

Coryell¹,

Beach²,

Robinson³

et al. 1950

J. Clin. Invest.

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The present investigation, an electrophoretic study of blood proteins, was planned to obtain information concerning the preconceptional physiologic status of women, the physiologic changes in the maternal organism in preparation for the exigencies of labor and losses at delivery, for the readjustment during the puerperium, and the demands of lactation. The results of this study will provide the basis for a better understanding of electrophoretic determinations of the proteins in the blood of women whose child-bearing was complicated by abnormal conditions or disease (1).While this investigation was in progress (2) the Tiselius procedure (3) for electrophoretic separation of blood proteins was applied to maternal, fetal, and infant sera by Longsworth, Curtis and Pembroke (4), Lagercrantz (5), Moore, DuPan and Buxton (6), and Scrimshaw and Alling (7) in studies of placental permeability and toxemias of pregnancy but, to our knowledge, this is the first report of electrophoretic determinations of constituents of the blood of healthy women throughout the reproductive cycle, from the preconceptional state through the postpartum readjustment period. The observations on cord blood will be presented in another communication. METHODS AND MATERIALSHealthy, well-nourished women in the early stages of gestation were selected from among his private patients 1 A preliminary report of the material in this paper was presented before the by the obstetrician (H. C. M.) on the basis of medical history and examination. The subjects were average or above in economic level and leading normal family lives. They were consuming good diets and clinically were considered well-nourished before, during, and after pregnancy. Since longitudinal data have greater value than cross-sectional data under known environmental conditions during growth (8) and when a physiologic process is involved (9), insofar as possible the same subjects were studied before conception, and throughout pregnancy, delivery, and postpartum, thus providing both types of results and minimizing difficulties in interpreting individual differences by using each subject as her own "control." Data were obtained with the same techniques for a group of non-pregnant women who had never been pregnant. Blood samnplinig and preparationBlood samples for chemical determinations of total protein and for electrophoresis were obtained during the three trimesters of pregnancy, at delivery, and within 24 hours, five to six days, and six to 12 weeks postpartum. Samples were obtained from finger punctures for evaluation of nutritional status. Since the subjects were living at home and blood samples were procured at times of examination by the obstetrician, the women were not always in a fasting state when blood was withdrawn.Blood was withdrawn from a vein in the antecubital fossa with minimum stasis which might affect the proteins (10), usually between 10:00 a.m. and 2:30 p.m. As accurately as possible, 20 ml. were drawn and discharged into a 50 ml., glass-stoppered Erlenmeyer flask contai...

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Section: And Discussionmentioning

confidence: 99%

Section: Electrophoretic Techniquementioning

confidence: 99%

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Metabolism of Women During the Reproductive Cycle. Xvii. Changes in Electrophoretic Patterns of Plasma Proteins Throughout the Cycle and Following Delivery 12

Coryell¹,

Beach²,

Robinson³

et al. 1950

J. Clin. Invest.

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“…4 Bromphenol blue migrated only with the more rapidly moving albumin A which is considered to be the normal type of albumin. Preliminary attempts to identify postalbumin by immunoelectrophoresis have been unsuccessful, and further study is in progress.…”

Section: Resultsmentioning

confidence: 98%

“…The concentration and electrophoretic pattern of serum proteins of the fetus differ from those of the adult in animals (1-3) as well as in man (4)(5)(6)(7). In early fetuses of some species, serum proteins have been described which are either absent or modified at later stages of development (8,9).…”

mentioning

confidence: 99%