An electrophoretic comparison of the enzymes of Saccharomyces yeasts.

Yamazaki, Masashi; Goto, Shoji; Komagata, Kazuo

doi:10.2323/jgam.29.305

Cited by 15 publications

(7 citation statements)

References 12 publications

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“…Our results differ from those obtained by other authors in studies on the electrophoretic mobility of isoenzymes in the species examined of the genus Saccharomyces as they generally present a greater intraspecific diversity in patterns and fail to separate the four species belonging to Succhuromyces senm stricto (Subden et al, 1982;Yamazaki et al, 1983;Poncet et al, 1992;Lewicka et al, 1995). The experimental conditions, namely the use of certified strains and the time of harvesting the cells, may account for the consistency of these results.…”

Section: Numerical Analysis Of Isoenzyme Profilescontrasting

confidence: 57%

“…1). In similar studies, based on a higher number of enzymes, the authors report as many different ETs as the number of strains under study (Subden et al, 1982;Yamazaki et al, 1983;Poncet et al, 1992). In contrast, Lewicka et al (1995) in studies of genetic diversity based on multilocus starch electrophoresis of seven isoenzymes described 11 different ETs for 27 strains of S. cerevisiae, which is in accordance with our results and indicates that the discriminatory power of the enzymes varies significantly.…”

Section: Resultsmentioning

confidence: 95%

“…Baptist & Kurtzman, 1976;Sidenberg & Lachance, 1986) and to type clinical isolates of pathogenic species (Brandt et a/., 1993 ;Doebbeling et al, 1993 ;Lehmann et al, 1989). The Saccharomyces sensu stricto group also received some attention, but either because only reference strains were used or because the identification of the strains was not authenticated by whole-Isoenzyme profiles in Saccharomyces sensu stricto genome comparisons, the conclusions were not sound and a clear separation of species was not achieved (Yamazaki et al, 1983;Lewicka et al, 1995). The purpose of the present work was to assess genetic variation among collection strains and new isolates belonging to species of the Saccharomyces sensu stricto group by the electrophoretic analysis of selected isoenzymes, and to further evaluate the usefulness of the technique for a rapid and sensitive identification of strains from this industrially important group of yeasts.…”

Section: Introductionmentioning

confidence: 99%

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Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto

Duarte

Pais

Spencer‐Martins

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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Genetic variation among 35 strains representing the four currently recognized species of Saccharomyces sensu stricto (Saccharomyces cerevisiae, Saccharomyces bayanus, Saccharomyces pastorianuslcarlsbergensis and Saccharomyces paradoxus) was estimated by analysing the electrophoretic mobilities of nonspecific esterases, acid phosphatase, lactate dehydrogenase and g I ucose-6-p hosphate de h ydrogenase isoenzymes. Twenty-two electrophoretic types were identified, a result in agreement with the phenotypic and genetic polymorphisms reported for this group of yeasts. However, the four species were clearly distinguishable based on the patterns obtained using three of the enzymes assayed, the resolving power not being improved by the introduction of data correspondent t o lactate dehydrogenase. The overall diversity was higher among S. cerevisiae isolates, in contrast with 5. paradoxus which showed only two patterns, one of which was common to four of the five strains studied. Concordant results from the application of the method and DNA hybridization experiments demonstrate its value for identification purposes.

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Section: Numerical Analysis Of Isoenzyme Profilescontrasting

confidence: 57%

Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto

Duarte

Pais

Spencer‐Martins

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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“…In these the detected number of enzymes was 3 or less, so they were excluded from the analysis on the ground that they would lead to noticeable error because of scarce input data. YAMAZAKI and KOMAGATA (8-10), YAMAZAKI et al (11,12), and YAMAZAKI and GoTo (13) reported that the electrophoretic comparison of enzymes was a useful tool for determining the taxonomical relations at the species level. Our research showed a high similarity value (87-100%) among the strains within the following species: 12 strains of Saccharomycopsis lipolytica, 4 of Saccharomycopsis fibuligera, 2 of Saccharomycopsis crataegensis, 2 of Stephanoascus ciferrii, 2 of Cephaloascus fragrans, and 2 of Guilliermondella selenospora.…”

Section: And Discussionmentioning

confidence: 99%

“…The reclassification of ascoideaceous yeasts has mainly been examined and discussed in terms of morphology, but scholars have yet to agree on all classifications (1,4,6,7). In recent years, YAMAZAKI and KOMAGATA (8-10), YAMAZAKI et al (11,12), and YAMAZAKI and GoTo (13) reported that electrophoretic comparison of enzymes is a useful tool for determining the taxonomic relations of various yeasts at the species level. YAMADA and KoNDO (14), YAMADA et al (15,16), and YAMADA and SMITH (17) reported that the coenzyme Q (ubiquinone) system was well correlated with the groupings of the ascomycetous yeast genera.…”

mentioning

confidence: 99%

Classification of ascoideaceous yeasts based on the electrophoretic comparison of enzymes and coenzymes Q systems.

Goto

Takami

1986

J. Gen. Appl. Microbiol.

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Sixty one strains of ascoideaceous yeasts belonging to twenty species of nine genera were examined from a taxonomical viewpoint based on a study of the electrophoretic comparison of the enzymes and the coenzymes Q (Co-Q) systems. The fifty strains, excluding those yeasts in which the detected number of enzymes was 3 or less, were divided into 4 clusters and 2 subclusters by numerical analysis of the relative electrophoretic mobilities (Rm) of 8 enzymes. On the other hand, the Co-Q systems of the ascoideaceous yeasts were Q-7, Q-8, or Q-9. These Co-Q systems and the septal ultrastructures coincided with each other. It is possible to divide these yeasts into four groups on the basis of similarity value according to the Rm of 8 enzymes: Saccharomycopsis lipolytica, other Saccharomycopsis spp. and related genera, Hyphopichia burtonii and/or Stephanoascus ciferrii, and Ambrosiozyma and Hormoascus. Saccharomycopsis lipolytica should be excluded from the genus Saccharomycopsis and is assigned to the genus Yarrowia. Ambrosiozyma and Hormoascus are considered to be cogeneric. Our data revealed close taxonomic relationship in the numerical classification based on the Rm of enzymes, Co-Q systems, and the septal ultrastructure.VoN ARx (1) described eight yeast genera in the Ascoideaceae Schroter which produce septate true mycelium, hat-shaped ascospores and blastoconidia. Stephanoascus ciferrii (2) was discovered later and the genus Hyphopichia was newly established as the teleomorph of Candida ciferrii (3). VoN ARx et al. (4) accepted the following genera in the Ascoideaceae. These were:

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Reductive biotransformations of organic compounds by saccharomyces cerevisiae: Study of oxidoreductases involved using electrophoretic zymograms

Young

Ward

1992

Biotech & Bioengineering

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The role of oxidoreductases in reduction of carbonyl compounds was investigated by application of zymogram techniques. Eight bands were observed using ethanol with nicotinamide adenine dinucleotide (NAD) as coenzyme. Bands observed with lactic acid and (R)-(-)-phenyl-1,2-ethanediol with nicotinamide adenine dinucleotide phosphate (NADP) had similar R values. 2-Hydroxyvalerate and malate manifested bands having similar R, values and were active with both NAD and NADP. Based on their structural similarity and identical R, values, oxidation of 1,Ccyclooctanediol (band #2) and cis-1,5-cyclooctanedioi may be due to a common enzyme. The PAGE-zymogram technique may be used on a preparative scale to facilitate purification and full characterization on the observed stained bands.

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An electrophoretic comparison of the enzymes of Saccharomyces yeasts.

Cited by 15 publications

References 12 publications

Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto

Distinctive electrophoretic isoenzyme profiles in Saccharomyces sensu stricto

Classification of ascoideaceous yeasts based on the electrophoretic comparison of enzymes and coenzymes Q systems.

Reductive biotransformations of organic compounds by saccharomyces cerevisiae: Study of oxidoreductases involved using electrophoretic zymograms

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