An electrophoretic comparison of enzymes in strains of the Q9-equipped species in the genus Sporobolomyces Kluyver et van Niel(Sporobolomycetaceae).

Abstract

A taxonomic study below the generic or at the specific level was made of electrophoretic patterns of seven enzymes in 18 strains of the Q9-equipped Sporobolomyces and Bensingtonia species. The seven enzymes were glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase, hexokinase, phosphoglucomutase, and fumarase. Seven species, S, intermedius, S. miscanthi, S. subroseus, S. yamatoanus, S. naganoensis, S, yuccicola, and S, phylladus, constituted clusters separate from each other (s = 0 or 14%). The two strains of S. miscanthi showing identical electrophoretic enzyme patterns (s= 100%) were linked to Sporobolomyces species NZ-3 with a similarity value of 29%. Within the five strains of S, yamatoanus examined, the type strain had a similarity value of 86°c with one other strain. The remaining three strains whose similarity values were calculated as 100% and 57°c were linked to the two strains including the type strain with a similarity value of 43%. The three strains of S. phylladus had identical electrophoretic enzyme patterns (s=100%). Sporobolomyces species NZ-24 had a separate cluster (s=0%). Sporobolomyces weijmanii was synonymous with S. intermedius (s =100%). Bensingtonia ciliata constituted its own cluster (s=0°0). These data are discussed from the taxonomic point of view.The genus Sporobolomyces Kluyver et van Niel was introduced for classifying red-pigmented ballistosporous yeasts (1). The type species of the genus was designated as Sporobolomyces roseus (2). YAMADA and KONDO (3,4) examined these ballistosporous yeasts for their Co-Q system and characterized them as having the

Bensingtonia ingoldii sp. nov., a ballistospore-forming yeast isolated from Knightia excelsa collected in New Zealand.

“…These characteristics coincide with the diagnosis of the genus Bensingtonia emended by Nakase and Boekhout (5). Yamada and Nakagawa (15) found that the electrophoretic pattern of 7 kinds of enzymes of this strain differed from those of any known Q-9-equipped species of Sporobolomyces and suggested that this strain represented a new species.…”

“…This strain contains no xylose in the whole cell hydrolyzate and has Q-9 as the major component of ubiquinones so that it is included in the "intermedius group," a group of species having Q-9 in the genus Sporobolomyces. Yamada and Nakagawa (15) found that this yeast is different from any known species of the Q-9-equipped species of Sporobolomyces in the electrophoretic pattern of seven enzymes. In 1988, Nakase and Boekhout (5) emended the diagnosis of the genus Bensingtonia and transferred all of the known Q-9-equipped species of Sporobolomyces into Bensingtonia.…”

Classification of heterobasidiomycetous yeasts: characteristics and affiliation of genera to higher taxa of Heterobasidiomycetes

“…Electrophoretic isozyme patterns are useful to differentiate species (Yamada et al 1986;Yamada and Nakagawa 1988;Komagata 1981, 1983) and have been used to analyse teleomorph-anamorph relationships. Dissimilarities of up to approximately 60% have been demonstrated to occur within species (Yamada et al 1986 Matsumoto 1988).…”

“…;Yamada and Nakagawa 1988;Nakase et al 1990). Two groups have been recognized within Mrakia (Yamada and Can.…”

Part XXIX. Significance of the coenzyme Q system in the classification of yeasts and yeast-like organisms. Bensingtonia phylladus, a new combination for the Q9-equipped species, Sporobolomyces phylladus.

“…However, several species equipped with the Q-9 system of the genus have been described.2~4) In a previous paper, 5) wehave reported the electrophoretic enzyme patterns of these Q9-equipped Sporobolomyces species in terms of taxonomy and recognized the following seven species: xylose in cell walls, the absence of episterol, and the presence of y-linolenic acid. The discrimination between the two genera, however, hinges on their Q-10 or Q-9 system and the presence or absence of carotenoids.…”