An electron microscopic study of ferritin

Farrant, J.

doi:10.1016/0006-3002(54)90376-5

Cited by 292 publications

(84 citation statements)

References 11 publications

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“…17, there are rings of evaporated metal with diameters of about 105 A around quadruplets (doublets or triplets), and there is a small space between the rings and the quadruplets (doublets or triplets). This is the characteristic image of isolated ferritin molecules, first demonstrated by Farrant and since confirmed (23,27). Thus, one may assume that the ferritin iron micelles in the unshadowed sections signify the presence of entire ferritin molecules.…”

Section: Furtker Identification Of Ferritin In Cells Of Animals Treatsupporting

confidence: 54%

“…Although in the previous work of Farrant (23) and of the author (11,12) it was shown that in electron micrographs the appearance of the iron hydroxide micelles of purified ferritin is characteristic, it was not shown that such micelles were regularly associated with the protein component of ferritin, the apoferritin. In the previous work, methods of chemical fractionation and of shadowcasting were combined with electron microscopy to identify ferritin obtained from tissues.…”

Section: Furtker Identification Of Ferritin In Cells Of Animals Treatmentioning

confidence: 94%

“…The crystals were characterized by means of procedures described by others (20,25), and were studied in the electron microscope as previously reported by the author (9)(10)(11)(12). Solutions of such crystals were also studied as previously reported (11,23).…”

Section: (B) Electron Diffractionmentioning

confidence: 95%

“…But intensities of reflections were estimated only by inspection of patterns. from opposite directions with chromium or palladium according to the method of Kahler and Lloyd (22) as applied to ferritin by Farrant (23).…”

Section: (B) Electron Diffractionmentioning

confidence: 99%

“…4, images recorded at a resolution of about 10 A reveal that the iron-dextran contains innumerable particles that are relatively dense to electrons, as well as short, filamentous structures of similar density. It may now be briefly recalled that the iron hydroxide micelles of individual ferritin molecules have a different structure in electron micrographs, since they appear as characteristic quadruplets of spherical dense particles (11,12,23,27), each of which measures about 30 A in diameter (Farrant: 27 A; Richter: 30 A). Each quadruplet as a whole thus has a diameter of about 60 A (Farrant: 55 A; Richter: 60 A).…”

Section: Structure and Identification Of The Iron Compounds By Means mentioning

confidence: 99%

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The Cellular Transformation of Injected Colloidal Iron Complexes Into Ferritin and Hemosiderin in Experimental Animals

Richter

1959

The Journal of Experimental Medicine

139

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Although there have been numerous studies on the fate of injected iron compounds in experimental animals (1-8), the transformation of the iron compounds within individual cells has not yet been traced at the molecular level. Recent work in which electron microscopy was combined with other techniques (9-12) has provided information on the molecular structure and the disposition of hemosiderin in several types of cells under varying circumstances, on the relationship of hemosiderin to ferritin, and on the possible role of specialized mitochondria ("siderosomes") in hemosiderosis. In the work now to be presented it has proved possible to distinguish intracellular deposits of iron compounds, given parenterally, from ferritin and from indigenous hemosiderin, and to gain insight into the transformation of the injected iron compounds into ferritin and hemosiderin. The findings bear on several aspects of iron metabolism, especially on the utilization of colloidal iron preparations within macrophages and endothelial cells. General Plan of ExperimentsThe general plan of the experiments was, first, to trace the sequence of changes in the fine structure of intracellular deposits of injected iron compounds in several types of cells in mice and rats at various intervals following intraperitoneal injection; second, to compare the physico-chemical nature of the intracellular material with that of the iron compounds prior to their injection; tkirdly, to confirm the actual presence of ferritin, about or in relation to deposits

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Section: Furtker Identification Of Ferritin In Cells Of Animals Treatsupporting

confidence: 54%