An Electron Microscopic Study of the Ductuli Efferentes and Rete Testis of the Guinea Pig

Ladman, Aaron J.; Young, William C.

doi:10.1083/jcb.4.2.219

Cited by 74 publications

(23 citation statements)

References 16 publications

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“…There are, however, some differences in the intracellular structure and content betw-een the non-ciliated cells of the ductuli efferentes proximales and ductuli efferentes distales, as show'll above in the Japanese quail, and in the domestic fowl [unpublished ultrastructural observations]. Ladman and Young [1958] have also shown that there are regional differences in the struc ture of the proximal and distal segments of the ductuli efferentes of the guinea pig.…”

Section: Discussionmentioning

confidence: 87%

The epididymal region of the Japanese quail (<i>Coturnix coturnix japonica</i>)

Aire¹

1979

Cells Tissues Organs

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The epididymal region of the Japanese quail was studied histologically. The organ consists of the extratesticular portion of the rete testis, the ductuli efferentes proximales and distales, the ducti conjugentes and ductus epididymidis. Distinct tubuli recti link the seminiferous tubules with the rete testis. The non-ciliated cells of the ductuli efferentes proximales and distales show, between them, certain internal structural differences which were highlighted. In 40% of the birds, the ductus deferens showed dark-grey pigments, regarded as melanin. The epididymal region was generally similar in structure to that of the domestic fowl, turkey and duck.

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Section: Discussionmentioning

confidence: 87%

The epididymal region of the Japanese quail (<i>Coturnix coturnix japonica</i>)

Aire¹

1979

Cells Tissues Organs

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“…Different hypotheses have been suggested to the origin and physiological role of the dark cells. [38] as well as [39] have considered it a modification of a single cell type at different phases of its metabolic cycle. [40][41][42][43][44] believed that, these cells are holocrine secretory cells as they lack stereocilia and have large clear vacuoles with finely granulated material.…”

Section: Discussionmentioning

confidence: 99%

Morphological Studies on the Epididymal Duct of the One-Humped Camel (Camelus Dromedaries)

Zayed¹,

Aly²,

Ibrahim³

et al. 2012

OJVM

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The present work was carried out on 20 epididymes of sexually mature one humped camel to elucidate the gross anatomical, scanning electron microscopical and light microscopical features of the epididymal duct. Anatomically, the epididymal duct of camel consists of three parts i.e. head, body and tail. Histomorphologically, the epididymal duct is subdivided into initial, middle and terminal segments, of which the middle segment is further subdivided into proximal, intermediate and distal parts. The epithelium lining the epididymal duct is ciliated pseudostratified columnar comprising mainly five cell types, namely principal, basal, apical, dark and halo cells. It displays numerous intraepithelial glands in the middle segment. In general, there is a gradual increase in the total diameter and decrease in the epithelial height of the epididymal duct from the initial to the terminal segments; that may, mechanically, facilities passage of the sperms toward the terminal segment. The lamina propria of the epididymal duct contains a layer of interlacing elastic fibers which may allow adequate elasticity of the duct that could help in its expansion. Moreover it is surrounded by numerous layers of circularly and obliquely arranged smooth muscle fibers which always increase in thickness toward the terminal segment.

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“…The capacity of this epithelium to absorb material from the lumen has been suggested in early studies (van Wagenen, 1925;Wagenseil, 1928;Young, 1933;Mason and Shaver, 1952). More recent electron-microscopic investigations performed with the use of electron-dense markers clearly indicated that the nonciliated epithelial cells readily incorporated these substances (Ladman and Young, 1958;Burgos et al, 1959;Holstein, 1964;Sedar, 1966, Morita, 1966Ladman, 1967;Yokoyama and Chang, 1971;Hrudka, 1980, 1981). Exhibiting numerous pinocytic vesicles, multivesicular bodies, and lysosomes, these epithelial cells appear suitably specialized to be effective endocytic cells (Montorzi and Burgos, 1967;Hoffer, 1972;Hamilton, 1975;Ramos and Dym, 1977;Flickinger et al, 1978;Jones et al, 1979).…”

mentioning

confidence: 88%

Endocytosis in nonciliated epithelial cells of the ductuli efferentes in the rat

1984

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The nonciliated cells lining the ductuli efferentes presented three distinct cytoplasmic regions. The apical region contained, in addition to cisternae of endoplasmic reticulum and mitochondria, two distinct membranous elements. The tubulovesicular system consisted of dilated tubules connected to the apical plasma membrane and subjacent distended vesicular profiles. The apical tubules, not connected to the cell surface, consisted of numerous densely stained tubules of small size which contain a compact, finely granulated material. The supranuclear region, in addition to a Golgi apparatus and ER cisternae, contained dilated vacuoles, pale and dense multivesicular bodies, as well as numerous dense granules identified cytochemically as lysosomes. The basal region contained the nucleus and many lipid droplets. The endocytic activity of these cells was investigated using cationic ferritin (CF) and concanavalin-A-ferritin (Con-A-ferritin) as markers of adsorptive endocytosis; and native ferritin (NF), concanavalin-A-ferritin in the presence of alpha-methyl mannoside, and horseradish peroxidase or albumin bound to colloidal gold for demonstrating fluid-phase endocytosis. These tracers were injected separately into the rete testis, and animals were sacrificed at various time intervals after injection. At 1 min, CF or Con-A-ferritin were seen bound to the apical plasma membrane, to the membrane of microvilli, and to the membrane delimiting elements of the tubulovesicular system. Between 2 and 5 min, these tracers accumulated in the densely stained apical tubules and at 15 min in the dilated vacuoles. Between 30 min and 1 hr, the tracers appeared in multivesicular bodies of progressively increasing density, whereas at 2 hr and later time intervals, many dense lysosomal elements became labeled. The tracers for fluid-phase endocytosis showed a distribution similar to that for CF or Con-A-ferritin except that they did not bind to the apical plasma membrane, microvilli, or membrane delimiting the tubulovesicular system. At no time interval were any of the tracers observed in the abluminal spaces. Thus, the nonciliated epithelial cells of the ductuli efferentes are actively involved in fluid-phase and adsorptive endocytosis, both of which result in the sequestration of endocytosed material within the lysosomal apparatus of the cell.

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An Electron Microscopic Study of the Ductuli Efferentes and Rete Testis of the Guinea Pig

Cited by 74 publications

References 16 publications

The epididymal region of the Japanese quail (<i>Coturnix coturnix japonica</i>)

The epididymal region of the Japanese quail (<i>Coturnix coturnix japonica</i>)

Morphological Studies on the Epididymal Duct of the One-Humped Camel (Camelus Dromedaries)

Endocytosis in nonciliated epithelial cells of the ductuli efferentes in the rat

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