An Electron Microscopic Study of Normal and Neoplastic Acidophil Cells of the Rat Pituitary

Schelin, U; Lundin, Per

doi:10.1530/acta.0.0670029

Cited by 16 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The other patient was known to have a high prolactin level and exhibited some acromegaloid types is indispensable in order to recognize these neoplasms. Our observations also suggest that the simultaneous occurrence of GH-and prolactin cells within the same neoplasm is not a matter of chance and the close relationship between the two cell types which was shown in animals (Schelin and Lundin, 1971) may exist in the human pituitary as well.…”

Section: •€Ppxsupporting

confidence: 70%

Ultrastructural Classification of Pituitary Adenomas

Horváth

Kovács

1976

Can. j. neurol. sci.

View full text Add to dashboard Cite

Section: •€Ppxsupporting

confidence: 70%

Ultrastructural Classification of Pituitary Adenomas

Horváth

Kovács

1976

Can. j. neurol. sci.

View full text Add to dashboard Cite

Immunochemical staining of the rat adenohypophysis in organ culture

1975

View full text Add to dashboard Cite

Rat anterior pituitaries were cytologically studied following cultivation in organ culture, with and without the addition of hypothalamic and cortical extracts. Although five distinct cell types could be identified with classical stains in the uncultivated glands, the peroxidase-labeled antibody technique (using antibodies against STH, LTH, FSH, LH and TSH) showed that not all of the immune-specific cell types were being identified with the classical stains. This discrepancy was magnified following culture as chromophilic cells seen with classic stains decreased in number with an increase in culture time. The peroxidase technique, however, revealed that all cells remained constant in type and number regardless of time in culture. While the addition of either hypothalamic or cortical extract to the culture medium produced cytological alterations demonstrated by the classical dyes, the antibody technique showed no such alterations. Such a comparison of staining techniques emphasizes the hazards of relying solely on histological procedures to reveal the hormonal activity of the pituitary gland.

show abstract

Heterogeneity of the MtTW₁₅ mammosomatotropic tumor. II. Characterization of parenchymal cells by superimposition immunocytochemistry and electron microscopy

1980

View full text Add to dashboard Cite

Large MtTw15 tumors, which secrete growth hormone (GH) and prolactin (PRL), are composed of ovoid, elongated, and angular cells which demonstrated interdigitating processes and junctional complexes. The majority of the cells were essentially agranular, but two types of granulated cells were identifiable. One class of granulated cells contained moderate to sparse populations of round dense-cored granules measuring up to 250 nm in diameter. Rod-shaped to filamentous mitochondria with an electron-dense matrix were characteristic of a second class of granulated cells with plemorphic granules of various sizes and electron densities. Images of exocytotic release of the round dense-cored granules were frequently seen, but were not observed with the pleomorphic granules, many of which were judged to be lysosomes. Superimposition immunocytochemistry revealed hormones only in the granulated cells with round to ovoid granules. Morphometry indicated that hormone specific subpopulations of tumor cells can be identified since PRL secretory granules were significantly smaller than GH secretory granules (149 +/- 6 nm for PRL versus 221 +/- 9 nm for GH, P less than 0.001). The vast majority of immunopositive cells contained only GH or PRL, but a few were observed containing both hormones. Ovoid to irregular-shaped nuclei, large lipid inclusions, numerous free ribosomes and polyribosomes, moderate development of the rough endoplasmic reticulum, and prominent Golig profiles were characteristics of all cell types. Irrespective of the presence or absence of cytoplasmic granular elements, particles resembling viruses were encountered in many tumor cells, and these frequently appeared to be budding into the cisternae of the endoplasmic reticulum.

show abstract

An Electron Microscopic Study of Normal and Neoplastic Acidophil Cells of the Rat Pituitary

Cited by 16 publications

References 0 publications

Ultrastructural Classification of Pituitary Adenomas

Ultrastructural Classification of Pituitary Adenomas

Immunochemical staining of the rat adenohypophysis in organ culture

Heterogeneity of the MtTW₁₅ mammosomatotropic tumor. II. Characterization of parenchymal cells by superimposition immunocytochemistry and electron microscopy

Contact Info

Product

Resources

About

An Electron Microscopic Study of Normal and Neoplastic Acidophil Cells of the Rat Pituitary

Cited by 16 publications

References 0 publications

Ultrastructural Classification of Pituitary Adenomas

Ultrastructural Classification of Pituitary Adenomas

Immunochemical staining of the rat adenohypophysis in organ culture

Heterogeneity of the MtTW15 mammosomatotropic tumor. II. Characterization of parenchymal cells by superimposition immunocytochemistry and electron microscopy

Contact Info

Product

Resources

About

Heterogeneity of the MtTW₁₅ mammosomatotropic tumor. II. Characterization of parenchymal cells by superimposition immunocytochemistry and electron microscopy