An Electron Microscopic Study of the Chorioallantoic Membrane Following Infection With Rous Sarcoma Virus

Sweeny, P. R.; Bather, R.

doi:10.1083/jcb.36.2.299

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…This observation agrees with our results of the strong proliferation of anx-1-expressing CC cells in the chorionic epithelium in response to grafted tumor spheroids; this might represent part of the reaction of the chicken CAM to invading pathogens (Sweeny and Bather 1968) or experimental intervention. Interestingly, degenerated cells in the CAM (similar to those that we have seen) have previously been identified as apoptotic endothelial cells that perish after exposure to pathogens (Sweeny and Bather 1968). We would also like to mention that anx-2 is found in endothelial cells of many tissues (Kim and Hajjar 2002), similar to the findings of the present study.…”

Section: Discussionsupporting

confidence: 92%

Annexins as cell-type-specific markers in the developing chicken chorionallantoic membrane

et al. 2005

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Between day E8 and E12 of embryonic development, the chicken chorioallantoic membrane (CAM) undergoes massive structural rearrangement enabling calcium-uptake from the eggshell to supply the growing embryo. However, the contribution of the various cell types of the chorionic epithelium including the capillary covering (CC) cells, villus cavity (VC) cells, endothelial-like cells, and basal cells to this developmental program is largely unknown. In order to obtain markers for the different cell types in the chorionic epithelium, we determined the expression patterns of various calcium-binding annexins in the developing chicken CAM. By reverse transcription/polymerase chain reaction with primers deduced from nucleotide sequences available in various databases, the presence of annexin (anx)-1, anx-2, anx-5, and anx-6 was demonstrated at days E8 and E12. Quantitative immunoblotting with novel antibodies raised against the recombinant proteins revealed that anx-1 and anx-5 were significantly up-regulated at day E12, whereas anx-2 and anx-6 expression remained almost unchanged in comparison to levels at day E8. Immunohistochemistry of paraffin-embedded sections of E12 CAM revealed anx-1 in CC cells and VC cells. Anx-2 was localized in capillaries in the chorionic epithelium and in basal cells of the allantoic epithelium, whereas anx-6 was detected in basal cells or endothelial-like cells of the chorionic epithelium and in the media of larger vessels in the mesenchyme. A 2-day exposure of the CAM to a tumor cell spheroid resulted in strong proliferation of anx-1-expressing CC cells suggesting that these cells participate in the embryonic response to experimental intervention. Thus, annexins exhibit complementary expression patterns and represent appropriate cell markers for the further characterization of CAM development and the interpretation of results obtained when using CAM as an experimental model.

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Section: Discussionsupporting

confidence: 92%

Annexins as cell-type-specific markers in the developing chicken chorionallantoic membrane

et al. 2005

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“…The allantoic layer did not appear to be altered. Such a pattern, investigated at the electron microscopic level, was found to be consistent with previous findings (Ganote et al, 1964;Sweeny and Bather, 1968). DISCUSSION Histologically, the pock of 12-day-old chick embryo CAMs infected with the avian reovirus belongs to the group of proliferative lesions with a central necrotic crater, described by Burnet (1955) for viruses such as vaccinia, extromelia and infectious laryngotracheitis.…”

Section: Resultssupporting

confidence: 78%

Ultrastructural histopathology of chick embryo chorioallantoic membrane infected with an avian reovirus

et al. 1980

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SUMMARYThe histopathological pattern of chick embryo chorioallantoic membrane infected by an avian reovirus, Crawley strain, has been investigated by semithin sections and transmission electron microscopy of ultrathin sections. The typical virus-induced pock is made up of a crust of necrotic ectodermal cell elements, an ectodermal proliferation and a proliferated, infiltrated, and oedematous mesoderm. Large crystals of virions are present in the ectodermal cells of the pock, where the details of the viral morphogenesis are not clearly revealed. While the virus appears neither to replicate in the mesodermal elements, nor in endodermal cells, it actively replicates in macrophages inside blood vessels. This finding may account for the dissemination of the infection.

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“…Pericytes, which are located on the mesenchymal side of the capillaries on the 14t~21st day of chick CAM development (Hoshi and Mori 1971;Wangensteen and Weibel 1982) cannot be distinguished from their mesenchymal precursor cells at earlier stages. Our so-called endothelial-like cells could as well correspond to the cells characterized as ~ by Shumko et al (1988) and Sweeny and Bather (1968). They are present during all stages of CAM development.…”

Section: Discussionmentioning

confidence: 99%

Evidence for intussusceptive capillary growth in the chicken chorio-allantoic membrane (CAM)

1993

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The aim of our investigations was to test whether the chicken chorio-allantoic membrane (CAM) could be an adequate in vivo model for a new mode of capillary growth, originally described in the rat lung and termed intussusceptive microvascular growth. According to that concept the capillary system does not grow by sprouting of vessels, but expands by insertion of transcapillary tissue pillars or posts which form new intercapillary meshes. In the present study, we observed slender transcapillary tissue pillars with diameters around 1 microns in the CAM by in vivo microscopy, and analyzed their ultrastructure by transmission electron microscopic investigation of serial sections. The pillars corresponded in size to those previously described in rat lung microvasculature. On day 7, the pillar core contained endothelial-, endothelial-like cells and collagen fibers, and on day 12 additionally chorionic epithelial cells. As a hypothesis we propose that slender cytoplasmic extensions of endothelial cells, heavily interdigitated in the post area and often projecting into the vascular lumen, could initiate the first step of pillar formation, i.e., interconnect opposite capillary walls. During both stages of development endothelial-like cells were observed in close relationship with the pillars. These cells seem to be relevant for tissue post completion and growth, as they were found to invade the core of the pillars. From the localization of the interendothelial junctions in the post region, a certain similarity to the concept proposed for the lung can be found. The observations confirm that the CAM is a very suitable material for the in vivo investigation of intussusceptive capillary growth.

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An Electron Microscopic Study of the Chorioallantoic Membrane Following Infection With Rous Sarcoma Virus

Cited by 9 publications

References 21 publications

Annexins as cell-type-specific markers in the developing chicken chorionallantoic membrane

Annexins as cell-type-specific markers in the developing chicken chorionallantoic membrane

Ultrastructural histopathology of chick embryo chorioallantoic membrane infected with an avian reovirus

Evidence for intussusceptive capillary growth in the chicken chorio-allantoic membrane (CAM)

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