An electron microscope study of the perfusion-fixed spleen

Pictet, Raymond; Orci, Lelio; Forssmann, W. G.; Girardier, L

doi:10.1007/bf00335215

Cited by 58 publications

(6 citation statements)

References 30 publications

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“…In the reticular meshwork of the rat spleen, very minute elastic fibers could be clearly identified in this study. Elastic fibers have been detected previously by electron microscopy in the trabeculae of the rat spleen (Pictet et al, 1969), but not in the red and white pulp, except in Saito's study (1977).…”

Section: Identification Of the Elastic Fibersmentioning

confidence: 84%

“…In fact, reticular fibers in both red and white pulp contain lamina-densalike material, collagenous fibrils, microfibrils, elastic fibers, and unmyelinated nerve fibers with Schwann cells; they lack only the smooth muscle cells. Several studies found that in the spleens of monkey (Galindo and Freeman, 1963), rat (Pictet et al, 1969), rabbit (Burke and Simon 19701, guinea pig (Janout and Weiss, 19721, human (Blue and Weiss, 1981b), and dog (Weiss et al, 1985), reticular fibers contained collagen fibers as the only fibrous components. The existence of elastic fibers in the reticular fibers of the rat spleen was reported by Saito (1977).…”

Section: Reticular Fibers In the Splenic Pulpmentioning

confidence: 97%

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Reticular meshwork of the spleen in rats studied by electron microscopy

et al. 1988

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The reticular meshwork of the rat spleen, which consists of both fibrous and cellular reticula, was investigated by transmission electron microscopy. The fibrous reticulum of the splenic pulp is composed of reticular fibers and basement membranes of the sinuses. These reticular fibers and basement membranes are continuous with each other. The reticular fibers are enfolded by reticular cells and are composed of two basic elements: 1) peripheral basal laminae of the reticular cells, and 2) central connective tissue spaces in which microfibrils, collagenous fibrils, elastic fibers, and unmyelinated adrenergic nerve fibers are present. The basement membranes of the sinuses are sandwiched between reticular cells and sinus endothelial cells and are composed of lamina-densalike material, microfibrils, collagenous fibrils, and elastic fibers. The presence of these connective tissue fibrous components indicates that there are connective tissue spaces in these basement membranes. The basement membrane is divided into three parts: the basal lamina of the reticular cell, the connective tissue space, and the basal lamina of the sinus endothelial cell. When the connective tissue space is very small or absent, the two basal laminae may fuse to form a single, thick basement membrane of the splenic sinus wall. The fibrous reticulum having these structures is responsible for support (collagenous fibrils) and rebounding (elastic fibers). The cells of the cellular reticulum--reticular cells and their cytoplasmic processes, which possess abundant contractile microfilaments, dense bodies, hemidesmosomes, basal laminae, and a well-developed, rough-surfaced endoplasmic reticulum, and Golgi complexes, which are characteristic of both fibroblasts and smooth muscle cells--are considered to be myofibroblasts. They may play roles in splenic contraction and in fibrogenesis of the fibrous reticulum. The contractile ability may be influenced by the unmyelinated adrenergic nerve fibers that pass through the reticular fibers. The three-dimensional reticular meshwork of the spleen consists of sustentacular fibrous reticulum and contractile myofibroblastic cellular reticulum. This meshwork not only supports the organ but also contributes to a contractile mechanism in circulation regulation, in collaboration with major contractile elements in the capsulo-trabecular system.

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Section: Identification Of the Elastic Fibersmentioning

confidence: 84%

Section: Reticular Fibers In the Splenic Pulpmentioning

confidence: 97%

Reticular meshwork of the spleen in rats studied by electron microscopy

et al. 1988

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“…Whatever the secretion manner of the reticular cells may be, these are fibroblastic cells, and SIMON andPICTET (1964), MOORE et al (1964) and PICTET et al (1969) have called the splenic reticular cells "fibroblasts" because of their cytological similarities to the usual fibroblasts. However, they are somewhat different from typical fibroblasts in the following aspects; (1) reticular cells are so closely and limitedly associated with strands of the extracellular reticulum, (2) in the limited areas they have basal lamina-like materials which are not observed around fibroblasts, (3) bundles of fine filaments are abundant throughout the cytoplasm and among these bundles, dense bodies, and beneath the plasma membranes, hemidesmosomes are prominent.…”

Section: Discussionmentioning

confidence: 99%

“…PICTET et al (1969) proposed a view that they might be the cellular skeleton and are found in all cells which seem required to function as a supporting framework.…”

Section: Discussionmentioning

confidence: 99%

“…A number of morphological studies on the reticular cells have dealt with their relation to the extracellular reticulum and have discussed their possible functions of fibrogenesis (SIMON and PICTET, 1964;MOORE et al, 1964;PICTET et al, 1969). Little tory mechanism of circulation in the organ as contractile cell elements, though a number of smooth muscle cells have long been known in the classic capsulo-trabecular system (BLOOM and FAWCETT, 1968).…”

Section: Mentsmentioning

confidence: 99%

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