An electrochemical sensor for fast detection of wound infection based on myeloperoxidase activity

Hajnsek, Martin; Schiffer, D; Harrich, Daniel; Koller, Daniel; Verient, Vanessa; Palen, Job v.d.; Heinzle, Andrea; Binder, Barbara; Sigl, Eva; Sinner, Frank; Guebitz, Georg M.

doi:10.1016/j.snb.2014.11.125

Cited by 32 publications

(17 citation statements)

References 44 publications

(53 reference statements)

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“…Inflammation precedes the infection process and due to the innate immune response, neutrophils are present in the wound site before the infection bursts. Elevated levels of almost all neutrophil-derived enzymes leads to new perspectives in screening of infection status [127][128][129] . The expected response of enzyme-based assays based on changing enzyme activity levels is rapid, simple (infected or non-infected) and may be detectable by naked eye (colour generation).…”

Section: Infection Biomarkersmentioning

confidence: 99%

Hydrogel Dressings for Advanced Wound Management

Francesko

Petkova

Tzanov

2019

CMC

177

134

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Composed in a large extent of water and due to their non-adhesiveness, hydrogels found their way to the wound dressing market as materials that provide a moisture environment for healing while being comfortable to the patient. Hydrogels' exploitation is constantly increasing after evidences of their even broader therapeutic potential due to resemblance to dermal tissue and ability to induce partial skin regeneration. The innovation in advanced wound care is further directed to the development of so-called active dressings, where hydrogels are combined with components that enhance the primary purpose of providing a beneficial environment for wound healing. The aim of this mini-review is to concisely describe the relevance of hydrogel dressings as platforms for delivery of active molecules for improved management of difficult-to-treat wounds. The emphasis is on the most recent advances in development of stimuli-responsive hydrogels, which allow for control over wound healing efficiency in response to different external modalities. Novel strategies for monitoring of the wound status and healing progress based on incorporation of sensor molecules into the hydrogel platforms are also discussed.

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Section: Infection Biomarkersmentioning

confidence: 99%

Hydrogel Dressings for Advanced Wound Management

Francesko

Petkova

Tzanov

2019

CMC

177

134

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“…Therefore, monitoring the wound pH can provide a forewarning of infection risks and an indicator of different healing phases, undoubtedly assisting effective wound management . In addition, the glucose level is another crucial factor that correlated to diabetic wound status and physical condition of patients . Variation in wound glucose concentration can reflect blood glucose level and serve as a prognostic indicator for diabetes, thus its control and modulation are therapeutically significant to guide the clinical treatment …”

Section: Introductionmentioning

confidence: 99%

A Multifunctional Pro‐Healing Zwitterionic Hydrogel for Simultaneous Optical Monitoring of pH and Glucose in Diabetic Wound Treatment

Zhu

Zhang

Song

et al. 2019

Adv Funct Materials

273

251

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Diabetic ulcer is the most common kind of chronic wound worldwide. Though great efforts have been devoted, diabetic ulcer still remains as a challenge that requires constant monitoring and management. In this work, a multifunctional zwitterionic hydrogel is developed to simultaneously detect two fluctuant wound parameters, pH and glucose level, to monitor the diabetic wound status. A pH indicator dye (phenol red) and two glucose sensing enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP), are encapsulated in the anti-biofouling and biocompatible zwitterionic poly-carboxybetaine (PCB) hydrogel matrix. The visible images are collected by a smartphone and transformed into RGB signals to quantify the wound parameters. Results show that the activity and stability of both two enzymes are improved within PCB hydrogel, and the K cat /K m value of PCB-HRP is ≈5.5 fold of free HRP in artificial wound exudate. This novel wound dressing can successfully monitor the pH range of 4-8 and glucose level of 0.1-10 × 10 −3 m. Meanwhile, it also provides a moist healing environment that can promote diabetic wound healing. This multifunctional wound dressing may open vistas in chronic wound management and guide the diabetes treatment in clinical applications.

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“…Some of these approaches are based on the detection of the enzymes present in the medium associated with an inflammatory response of the human body. 15,16,17,18 In different strategies, virulence factors secreted by pathogenic bacteria are targeted for the in situ detection. 19,20,21,22 Zakir Hossain et al 23 reported for instance on a lab-onpaper assay for the rapid and sensitive detection of E. coli in water.…”

Section: Introductionmentioning

confidence: 99%