An Electrochemical Nanosensor for Monitoring the Dynamics of Intracellular H<sub>2</sub>O<sub>2</sub> Upon NADH Treatment

Zhang, Shuai; Qin, Hancheng; Cheng, Shuwen; Zhang, Yue; Gao, Nan; Zhang, Meining

doi:10.1002/anie.202300083

Cited by 9 publications

(8 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With this nanosensor, they find that the level of intracellular H 2 O 2 increases with NADH treatment, and intratumoral injection of high-dose of NADH can inhibit tumor growth in mice, providing a potential therapeutic strategy for cancer therapy. [353] Proposing the concept of hybrid bacteria, Sun et al conjugated Dox onto the surface of a non-pathogenic E. coli strain overexpressing glucose dehydrogenase through a stable linker of amide bonds. More amounts of NADPH are produced in tumor sites by glucose dehydrogenase within E. coli, promoting the generation of toxic ROS within the tumor.…”

Section: Responsive Nanosystems Suppressing Reductive Stress To Indir...mentioning

confidence: 99%

Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy

Wu,

Zhou,

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Cancer cells typically display redox imbalance compared with normal cells due to increased metabolic rate, accumulated mitochondrial dysfunction, elevated cell signaling, and accelerated peroxisomal activities. This redox imbalance may regulate gene expression, alter protein stability, and modulate existing cellular programs, resulting in inefficient treatment modalities. Therapeutic strategies targeting intra‐ or extracellular redox states of cancer cells at varying state of progression may trigger programmed cell death if exceeded a certain threshold, enabling therapeutic selectivity and overcoming cancer resistance to radiotherapy and chemotherapy. Nanotechnology provides new opportunities for modulating redox state in cancer cells due to their excellent designability and high reactivity. Various nanomaterials are widely researched to enhance highly reactive substances (free radicals) production, disrupt the endogenous antioxidant defense systems, or both. Here, the physiological features of redox imbalance in cancer cells are described and the challenges in modulating redox state in cancer cells are illustrated. Then, nanomaterials that regulate redox imbalance are classified and elaborated upon based on their ability to target redox regulations. Finally, the future perspectives in this field are proposed. It is hoped this review provides guidance for the design of nanomaterials‐based approaches involving modulating intra‐ or extracellular redox states for cancer therapy, especially for cancers resistant to radiotherapy or chemotherapy, etc.

show abstract

Section: Responsive Nanosystems Suppressing Reductive Stress To Indir...mentioning

confidence: 99%

Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy

Wu,

Zhou,

et al. 2024

Advanced Science

View full text Add to dashboard Cite

show abstract

“…Electrochemical microsensors with excellent temporal and spatial resolution have been widely employed for in situ monitoring of biomolecules in vivo. − For instance, a carbon fiber microelectrode (CFME) functionalized with DNA was fabricated to trace nitric oxide release from cells and tissues . Aptamer-based CFMEs were designed for highly selective probing of neurochemical dynamics in living systems .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Monitoring of Sphingosine-1-phosphate-Induced ATP Release Using a Microsensor Based on an Entropy-Driven Bipedal DNA Walker

Jiang,

Liu,

Jia

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

Neuropathic pain is a chronic and severe syndrome for which effective therapy is insufficient and the release of ATP from microglia induced by sphingosine-1phosphate (S1P) plays a vital role in neuropathic pain. Therefore, there is an urgent demand to develop highly sensitive and selective ATP biosensors for quantitative monitoring of low-concentration ATP in the complex nervous system, which helps in understanding the mechanism involved in neuropathic pain. Herein, we developed an electrochemical microsensor based on an entropy-driven bipedal DNA walker. First, the microsensor specifically recognized ATP via ATP aptamers, initiating the entropydriven bipedal DNA walker. Subsequently, the bipedal DNA walker autonomously traversed the microelectrode interface, introducing methylene blue to the electrode surface and achieving cascade signal amplification. This microsensor showed excellent selectivity, stability, and a low limit of detection at 1.13 nM. The S1P-induced ATP release from BV2 cells was successfully monitored, and it was observed that dicumarol could inhibit this release, suggesting dicumarol as a potential treatment for neuropathic pain. The microsensor's small size exhibited significant potential for monitoring ATP level changes in neuropathic pain in vivo, which provides a new strategy for in situ and quantitative monitoring of nonelectroactive biomolecules associated with neurological diseases.

show abstract

“…However, there is a paucity of high-sensitivity biological sensors because the associated preparation conditions are often complex, costly, and potentially toxic and may suffer from stability issues. Traditional techniques for monitoring ROS, such as electron paramagnetic resonance, fluorescence, − chemiluminescence, , and electrochemical analysis, − typically have several limitations, including poor stability, low sensitivity, and cumbersome operation . Therefore, it is of utmost importance to develop new methods with high sensitivity and superior biocompatibility to measure ROS in complex biological environments.…”

Section: Introductionmentioning

confidence: 99%

Tri-mode Responses to Reactive Oxygen Species In Vivo by Chiral Vanadium-Based Nanoparticles

Yu,

Kuang,

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

Reactive oxygen species (ROS) are closely associated with the redox balance of the physiological environment, and monitoring ROS can aid in the early diagnosis of many diseases, including cancer. In this study, chiral vanadium trioxide/vanadium nitride (V2O3/VN) nanoparticles (NPs) modified with an organic dye (cyanine 3 [Cy3]) were prepared for ROS sensing. Chiral V2O3/VN NPs were prepared with the “ligand-induced chirality” strategy and showed a g-factor of up to 0.12 at a wavelength of 512 nm. To the best of our knowledge, this g-factor is the highest value of all chiral ceramic nanomaterials. The very high g-factor of the nanoprobe confers very high sensitivity, because the higher g-factor, the higher sensitivity. In the presence of ROS, V3+ in the chiral V2O3/VN nanoprobe undergoes a redox reaction to form V2O5, reducing the circular dichroism and absorbance signals, whereas the fluorescence signal of Cy3 is restored. With this nanoprobe, the limits of detection for the circular dichroic and fluorescence signals in living cells are 0.0045 nmol/106 and 0.018 nmol/106 cells, respectively. This chiral nanoprobe can also monitor ROS levels in vivo by fluorescence. This strategy provides an innovative approach to the detection of ROS and is expected to promote the wider application of chiral nanomaterials for biosensing.

show abstract

An Electrochemical Nanosensor for Monitoring the Dynamics of Intracellular H₂O₂ Upon NADH Treatment

Cited by 9 publications

References 71 publications

Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy

Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy

Electrochemical Monitoring of Sphingosine-1-phosphate-Induced ATP Release Using a Microsensor Based on an Entropy-Driven Bipedal DNA Walker

Tri-mode Responses to Reactive Oxygen Species In Vivo by Chiral Vanadium-Based Nanoparticles

Contact Info

Product

Resources

About

An Electrochemical Nanosensor for Monitoring the Dynamics of Intracellular H2O2 Upon NADH Treatment

Cited by 9 publications

References 71 publications

Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy

Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy

Electrochemical Monitoring of Sphingosine-1-phosphate-Induced ATP Release Using a Microsensor Based on an Entropy-Driven Bipedal DNA Walker

Tri-mode Responses to Reactive Oxygen Species In Vivo by Chiral Vanadium-Based Nanoparticles

Contact Info

Product

Resources

About

An Electrochemical Nanosensor for Monitoring the Dynamics of Intracellular H₂O₂ Upon NADH Treatment