An Electrochemical Immunosensor for Sensitive Detection of the Tumor Marker Carcinoembryonic Antigen (CEA) Based on Three-Dimensional Porous Nanoplatinum/Graphene

Jing, Aihua; Xu, Qian; Wei, Feng; Liang, Guozheng

doi:10.3390/mi11070660

Cited by 30 publications

(20 citation statements)

References 42 publications

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“…Memristive devices used in biosensing are usually functionalized with antibodies to detect antigens through a change in the voltage gap in the devices' response due to antibody-antigen interaction. Four main techniques exist in the literature for the attachment of antibodies on the surface of a device, namely direct adsorption [20], [45], [46], affinity approach [47], covalent attachment using Glycidoxypropyltrimethoxysilane (GPTS) [18,48,49], covalent attachment using 1-(3-(Dimethylamino)propyl)-3ethyl carbodiimide hydrochloride (EDC) and Nhydroxysulfosuccinimide (NHS) [16,[50][51][52][53][54][55], and noncovalent attachment [17]. The direct adsorption technique usually starts by treating the device with oxygen plasma for 15 minutes to increase hydroxyl groups (OH-).…”

Section: B Bio-functionalization Of Antibodiesmentioning

confidence: 99%

Memristive Biosensors for Cancer Biomarkers Detection: A Review

et al. 2023

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Detecting cancer biomarkers at an early stage at the clinical level has been the interest of numerous researchers over the years due to its impact on recovery. Therefore, attention is towards fabricating reliable, cost-effective, reproducible, and accurate devices for point-of-care screening. This review aims to highlight the emerging field of memristive biosensors and compare it to similar electrochemical devices used for cancer biomarker detection. The limit of detection (LOD) achieved by memristive biosensors was generally in the femtomolar (fM) range in comparison to field effect transistors (FET) and electrochemical immunosensors, which in most instances exhibited a LOD in the picomolar (pM) and nanomolar (nM) range. Most current memristive biosensors are fabricated using silicon nanowires, which calls for exploring different materials and structures that may lower fabrication complexity and increase reproducibility. This article examines the working principle of memristors for biosensing, the biofunctionalization of antibodies, the interaction between antibodies and antigens and its influence on memristors, as well as fabrication processes and applications of memristors for biosensing. This paper will report on memristor-based biomedical sensors focusing on cancer screening. In addition, the outlook of reduced graphene oxide (rGO) as an active material for sensing will be discussed. Memristors are anticipated to enhance the future of sensing due to their great sensitivity and simplicity of fabrication.

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Section: B Bio-functionalization Of Antibodiesmentioning

confidence: 99%

Memristive Biosensors for Cancer Biomarkers Detection: A Review

et al. 2023

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“…In such circumstances, although these infectious agents are present, they do not generate enough available Abs in the blood, so the concentration of the Abs in In this regard, Table 1 contains a list of recent researches in the literature of biosensors for medical applications using antibodies and aptamers as bioreceptors. It is worthy notable that these biomolecules facilitate the biosensing of analytes at concentrations as low as some femtograms per milliliter [86][87][88][89][90]. Note: *NPs = Nanoparticles…”

Section: Efficiency Of Immunosensors and Aptasensorsmentioning

confidence: 99%

Mof@Mos2-Based Electrochemical Biosensor for Ca125 Detection

Weifeng¹

2020

BJSTR

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Antibodies and aptamers play a crucial role to improve the analytical performance of biosensors for medical applications. Besides their natural compatibility with many antigens and pathogens, their biochemical structure selectively binds the analyte providing high sensitivity and selectivity. Accordingly, this minireview presents the recent approaches in the field of immunosensors and aptasensors for clinical diagnoses with a focus on the key features of sensors over the conventional diagnostic assays and the trends in this field of knowledge (point-of-care devices for in situ applications, label-free bioreceptors, real-time monitoring of analytes and outstanding transduction techniques).

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“…If this indicator is significantly elevated, it can indicate a gastrointestinal tumor. The increase in CA19-9 is especially significant in gastric cancer with liver metastasis and pancreatic metastasis, which is better than other tumor markers [ 5 , 6 , 7 , 8 ]. Clinical methods such as chemiluminescence (CL) and electrochromic luminescence (ECL) are mainly used to detect CA19-9 [ 9 , 10 , 11 , 12 ], but there are shortcomings such as expensive reagents and cumbersome operation.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer

et al. 2022

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A CA19-9 electrochemical immunosensor was constructed using a hybrid self-assembled membrane modified with a gold electrode and applied to detect real samples. Hybrid self-assembled membranes were selected for electrode modification and used to detect antigens. First, the pretreated working electrodes were placed in a 3-mercaptopropionic acid (MPA)/β-mercaptoethanol (ME) mixture for 24 h for self-assembly. The electrodes were then placed in an EDC/NHS mixture for 1 h. Layer modification was performed by stepwise dropwise addition of CA19-9 antibody, BSA, and antigen. Differential pulse voltammetry was used to characterize this immunosensor preparation process. The assembled electrochemical immunosensor enables linear detection in the concentration range of 0.05–500 U/mL of CA19-9, and the detection limit was calculated as 0.01 U/mL. The results of the specificity measurement test showed that the signal change of the interfering substance was much lower than the response value of the detected antigen, indicating that the sensor has good specificity and strong anti-interference ability. The repeatability test results showed that the relative standard deviations were less than 5%, showing good accuracy and precision. The CA19-9 electrochemical immunosensor was used for the actual sample detection, and the experimental results of the standard serum addition method showed that the RSD values of the test concentrations were all less than 10%. The recoveries were 102.4–115.0%, indicating that the assay has high precision, good accuracy, and high potential application value.

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An Electrochemical Immunosensor for Sensitive Detection of the Tumor Marker Carcinoembryonic Antigen (CEA) Based on Three-Dimensional Porous Nanoplatinum/Graphene

Cited by 30 publications

References 42 publications

Memristive Biosensors for Cancer Biomarkers Detection: A Review

Memristive Biosensors for Cancer Biomarkers Detection: A Review

Mof@Mos2-Based Electrochemical Biosensor for Ca125 Detection

Electrochemical Immunoassay for Tumor Marker CA19-9 Detection Based on Self-Assembled Monolayer

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