An electrochemical immunosensor for prostate specific antigen using nitrogen-doped graphene as a sensing platform

Meng, Wenwen; Zhang, Wenjuan; Zhang, Junjun; Chen, Xi; Zhang, Yu-Zhong

doi:10.1039/c9ay00064j

Cited by 23 publications

(6 citation statements)

References 49 publications

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“…This modification was demonstrated by Meng and colleagues who replaced the detection antibody with a PSA-specific aptamer labeled with ferrocene (Fc). 23 In the presence of the target, an Ab 1 /PSA/Apt 2 -Fc sandwich was formed, and the electrochemical response of Fc was recorded using DPV. This assay displayed a linear detection range of 0.05-100 ng ml −1 with a LOD of 0.017 ng ml −1 and a human serum recovery rate of 96.8%-101.4%.…”

Section: Detection Mechanismsmentioning

confidence: 99%

Review—Recent Advances in Electrochemical Detection of Prostate Specific Antigen (PSA) in Clinically-Relevant Samples

Traynor

Pandey

Maclachlan

et al. 2020

J. Electrochem. Soc.

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Electrochemical biosensors hold great promise for enabling clinical analysis of biomarkers at the point-of-care. This is particularly of interest for cancer management due to the importance of early diagnostics as well as the critical need for frequent treatment monitoring. We have reviewed clinically-relevant electrochemical biosensors that have been developed over the past five years for the analysis of prostate specific antigen (PSA), a model protein target for prostate cancer management. We have critically evaluated the key performance metrics of these biosensors for clinical translation: limit-of-detection, linear range, and recovery rate in bodily fluids. These PSA electrochemical biosensors can be broadly categorized as sandwich assays, direct detection assays, and indirect detection assays. Among these, indirect detection assays deliver the lowest limit-of-detection. We have identified the development of multiplexed assays for detecting a panel of cancer biomarkers that includes a combination of protein and nucleic acids targets as a key priority for future development.

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Section: Detection Mechanismsmentioning

confidence: 99%

Review—Recent Advances in Electrochemical Detection of Prostate Specific Antigen (PSA) in Clinically-Relevant Samples

Traynor

Pandey

Maclachlan

et al. 2020

J. Electrochem. Soc.

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“…Prostate specific antigen (PSA) is the best serum marker for the detection of prostatic carcinoma [21]. When its content in serum is > 4 ng mL À 1 , people can be considered pathologic, and when it exceeds 10 ng mL À 1 , people will be regarded as a patient with prostate cancer [22]. So it is necessary to provide a robust technique for the diagnosis of prostate cancer by detecting PSA.…”

Section: Introductionmentioning

confidence: 99%

A Photoelectrochemical Immunosensor for Prostate Specific Antigen Detection Based on Graphdiyne Oxide Conjugated with Horseradish Peroxidase

Wang

Xiao

et al. 2020

Electroanalysis

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Graphdiyne (GDY) was a novel flat material with sp and sp2 hybridized carbon atoms. It exhibited good biocompatibility. The application of GDY in PEC immunosensor was very limited. Thus, a novel photoelectrochemical sensor for the sensitive detection of prostate specific antigen (PSA) was proposed by using GDY oxide (GDYO) conjugated with horseradish peroxidase (HRP) and secondary antibody for photocurrent signal inhibition. GDYO was prepared by oxidation of honeycomb‐like nanotubes composed of numerous GDY nanosheets. It showed high loading capacity for HRP and the catalytic activity of HRP could be remained. With reduced graphene oxide‐CdS (rGO‐CdS) as photoelectrochemical sensing platform, a sandwich‐type photoelectrochemical (PEC) immunosensor was thus fabricated. The immunosensor presented a wide linear concentration range of 10 fg mL−1–20.0 ng mL−1 with a detection limit (LOD) of 3.5 fg mL−1. Moreover, the PEC immunosensor displayed ideal reproducibility, stability, and selectivity, which was a promising platform for the detection of other important tumor targets.

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“…Unfortunately, these standard methodologies have high cost, long analysis times (around 18 h) and require pretreatment of samples [5–6]. Other approaches to produce immunosensors have therefore been studied, including electroanalytical methods [7–8] in which antibodies or antigens are immobilized on a suitable matrix and the specific antigen–antibody recognition leads to an electrical or electrochemical signal.…”

Section: Introductionmentioning

confidence: 99%

Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

Proença¹,

Freitas²,

Baldo³

et al. 2019

Beilstein J. Nanotechnol.

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Diagnosis of cancer using electroanalytical methods can be achieved at low cost and in rapid assays, but this may require the combination with data treatment for determining biomarkers in real samples. In this paper, we report an immunomagnetic nanoparticle-based microfluidic sensor (INμ-SPCE) for the amperometric detection of the prostate-specific antigen (PSA) biomarker, the data of which were treated with information visualization methods. The INμ-SPCE consists of eight working electrodes, reference and counter electrodes. On the working electrodes, magnetic nanoparticles with secondary antibodies with the enzyme horseradish peroxidase were immobilized for the indirect detection of PSA in a sandwich-type procedure. Under optimal conditions, the immunosensor could operate within a wide range from 12.5 to 1111 fg·L−1, with a low detection limit of 0.062 fg·L−1. Multidimensional projections combined with feature selection allowed for the distinction of cell lysates with different levels of PSA, in agreement with results from the traditional enzyme-linked immunosorbent assay. The approaches for immunoassays and data processing are generic, and therefore the strategies described here may provide a simple platform for clinical diagnosis of cancers and other types of diseases.

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An electrochemical immunosensor for prostate specific antigen using nitrogen-doped graphene as a sensing platform

Abstract: In this study, a sensitive electrochemical immunosensor for prostate specific antigen (PSA) detection is described using nitrogen-doped graphene (NG) and gold nanoparticles (Au NPs) as a sensing interface.

Cited by 23 publications

References 49 publications

Review—Recent Advances in Electrochemical Detection of Prostate Specific Antigen (PSA) in Clinically-Relevant Samples

Review—Recent Advances in Electrochemical Detection of Prostate Specific Antigen (PSA) in Clinically-Relevant Samples

A Photoelectrochemical Immunosensor for Prostate Specific Antigen Detection Based on Graphdiyne Oxide Conjugated with Horseradish Peroxidase

Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

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