An electrochemical immunosensor for brain natriuretic peptide prepared with screen-printed carbon electrodes nanostructured with gold nanoparticles grafted through aryl diazonium salt chemistry

Serafín, Verónica; Torrente‐Rodríguez, Rebeca M.; González‐Cortés, Araceli; Frutos, Pablo García de; Sabaté, Manel; Campuzano, Susana; Yáñez‐Sedeño, Paloma; Pingarrón, José M.

doi:10.1016/j.talanta.2017.10.063

Cited by 58 publications

(27 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After blocking with casein and biotin, as well as in the further steps, the R CT value significantly increased and two semicircles appeared in the Nyquist plots then fitting the experimental data to the equivalent circuit shown on top of Figure S4A2 (the R CT values were 550 Ω for the semicircle at high frequencies and 3,070 Ω for the two semicircles). This behavior is characteristic of heterogeneous electrode coatings with more than one layer and it is usual for immunosensors involving incubation with casein . A slight increase in R TC was observed after the antigen‐antibody affinity reaction (640 Ω for the semicircle at high frequencies and 3,180 Ω for the total of the two semicircles).…”

Section: Resultsmentioning

confidence: 81%

“…This behavior is characteristic of heterogeneous electrode coatings with more than one layer and it is usual for immunosensors involving incubation with casein. [41,42,55] A slight increase in R TC was observed after the antigen-antibody affinity reaction (640 Ω for the semicircle at high frequencies and 3,180 Ω for the total of the two semicircles). The incorporation of the HRP-DAb-AuNPs/MWCNTs conjugate led to a decrease in the R CT (460 Ω for the semicircle at high frequencies and 2,420 Ω for the two semicircles) as a consequence of the high conductivity and fast electron transfer allowed by MWCNTs and AuNPs.…”

Section: Electrochemical Stepwise Characterization Of Rankl Immunosensormentioning

confidence: 98%

“…As it is shown in Figure 3A, while no reduction or oxidation peaks were observed at unmodified SPCE and MWCNTs/SPCE, voltammograms recorded at the AuNPs/SPCE and AuNPs/MWCNTs/ SPCE showed the characteristic anodic peak corresponding to the formation of gold oxides and the cathodic peak associated to their further reduction to metallic gold, which again confirms the presence of gold in the hybrid nanomaterial. [55] With the purpose to evaluate also the electrochemical behavior of the enzymatic mediator HQ, which was further used in the RANKL amperometric immunosensor, CVs of 1 mM HQ solution prepared in 50 mM PB pH 6.0, were recorded at SPCEs modified with AuNPs, MWCNTs, or AuNPs/MWCNTs ( Figure 3B). As expected, HQ showed slightly larger cathodic peak currents (À 23 vs. À 18 μA), and smaller ΔE (427 vs. 560 mV) at the AuNPs/SPCE than at the unmodified SPCE.…”

Section: Characterization Of Aunps/mwcntsmentioning

confidence: 99%

See 2 more Smart Citations

Carbon/Inorganic Hybrid Nanoarchitectures as Carriers for Signaling Elements in Electrochemical Immunosensors: First Biosensor for the Determination of the Inflammatory and Metastatic Processes Biomarker RANK‐ligand

et al. 2020

View full text Add to dashboard Cite

This work reports the development of the first integrated electrochemical immunosensor for the determination of the ligand receptor activator nuclear factor‐κB (RANKL), a biomarker that plays an important role in the regulation of bone resorption process and, as reported recently, in oncology by modulating the immune system response facilitating the tumor growth and metastatic process during cancer development. The developed platform involves a sandwich‐type immunoassay with covalent immobilization of biotinylated capture antibodies onto streptavidin/4‐aminobenzoic acid (p‐ABA) grafted screen‐printed carbon electrodes (bCAb‐Strep/p‐ABA‐SPCE) and the use of an hybrid nanomaterial composed of gold nanoparticles (AuNPs) and multi‐walled carbon nanotubes (MWCNTs) as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the H2O2/hydroquinone (HQ) system allowed the construction of a linear calibration plot for RANKL standards between 10.4 and 1,000 pg mL−1 with a LOD of 3.1 pg mL−1. The determination implied simple handling protocols and short assay times. The developed immunosensor was applied to the determination of RANKL in human serum of patients diagnosed with colorectal cancer (CRC) or rheumatoid arthritis (RA). Each determination required only 5 μL of undiluted serum and took less than 2 h counting since the preparation of blocked‐bCAb‐Strep/p‐ABA‐SPCE.

show abstract

Section: Resultsmentioning

confidence: 81%

Section: Electrochemical Stepwise Characterization Of Rankl Immunosensormentioning

confidence: 98%

Section: Characterization Of Aunps/mwcntsmentioning

confidence: 99%

See 1 more Smart Citation

Carbon/Inorganic Hybrid Nanoarchitectures as Carriers for Signaling Elements in Electrochemical Immunosensors: First Biosensor for the Determination of the Inflammatory and Metastatic Processes Biomarker RANK‐ligand

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Recent advances in this area include the fabrication of 3D nanostructured electrodes with high surface area and electro-chemical activity where this structure was induced by freeze drying a self-assembled reduced graphene oxide ink [111]. Further advances include the addition of specific polymer and small molecule co-absorbers in specific ratios that interact with the carbon inks to direct intermolecular forces and create free-standing electrodes with either a high mechanical integrity or high electrical conductivity and surface area depending on the additive ratio [112][113][114].…”

Section: 4printable Electrode Materialsmentioning

confidence: 99%

Manipulating nanoscale structure to control functionality in printed organic photovoltaic, transistor and bioelectronic devices

et al. 2019

View full text Add to dashboard Cite

Printed electronics is simultaneously one of the most intensely studied emerging research areas in science and technology and one of the fastest growing commercial markets in the world today. For the past decade the potential for organic electronic (OE) materials to revolutionize this printed electronics space has been widely promoted. Such conviction in the potential of these carbonbased semiconducting materials arises from their ability to be dissolved in solution, and thus the exciting possibility of simply printing a range of multifunctional devices onto flexible substrates at high speeds for very low cost using standard roll-to-roll printing techniques. However, the transition from promising laboratory innovations to large scale prototypes requires precise control of nanoscale material and device structure across large areas during printing fabrication. Maintaining this nanoscale material control during printing presents a significant new challenge that demands the coupling of OE materials and devices with clever nanoscience fabrication approaches that are adapted to the limited thermodynamic levers available. In this review we present an update on the strategies and capabilities that are required in order to manipulate the nanoscale structure of large area printed organic photovoltaic (OPV), transistor and bioelectronics devices in order to control their device functionality. This discussion covers a range of efforts to manipulate the electroactive ink materials and their nanostructured assembly into devices, and also device processing strategies to tune the nanoscale material properties and assembly routes through printing fabrication. The review finishes by highlighting progress in printed OE devices that provide a feedback loop between laboratory nanoscience innovations and their feasibility in adapting to large scale printing fabrication. The ability to control material properties on the nanoscale whilst simultaneously printing functional devices on the square metre scale is prompting innovative developments in the targeted nanoscience required for OPV, transistor and biofunctional devices.

show abstract

“…The 1 H and 13 C NMR spectra and elemental analysis (CHN) are nicely monitored the successful formation of the bis-Imi. 1 Figure 1(a) shows the presence of imidazolium proton H2′ signal (N-CH-N) at chemical shifts of 7.7 ppm. This chemical shift is consistent with the chemical shifts of common imidazolium salts [9].…”

Section: Synthesis Of Bis-imimentioning

confidence: 99%

In-situ Nitrous Acid Generation over Silica Imidazole Catalyst for Dyes Production

Hello

Fahad

2018

Bull. Chem. React. Eng. Catal.

View full text Add to dashboard Cite

The objective of this research is to prepare a new type of heterogeneous catalyst and to study its usage for in-situ nitrous acid generation to form a diazonium salt. The high pure silica (> 95%) was produced by burning the clean rice husk at 800 °C. After that, the silica was transferred to sodium silicate using 1.0 M of NaOH, followed by immobilizing with 3-(chloropropyl)triethoxysilane in a simple one-pot synthesis. Finally, the material was refluxed with (0.015 mol) of p-xylyl di-imidazolium chloride. The silicon solid-state nuclear magnetic resonance shows the Q 4 , Q 3 , T 3 , and T 2 chemical shifts at expected position. Carbon solid-state nuclear magnetic resonance spectrum shows different peaks at different chemical shifts related to the carbon structures of the organic moieties. The catalyst is stable up to 277 ºC according to the thermal analysis. TEM images show smooth and porous regularly shaped particles with an estimation size of ca. 5 nm. Coupling reaction of aromatic compounds was carried out with a diazonium salt of aniline to yield a monoazo dye. All dyes were showed matching the elemental analysis with the theoretical calculation. Besides this, the spectrum of FT-IR and UV-Visible were recorded. The catalyst was stable, easy separation from the reaction mixture, and reusable by a simple experimental procedure. The catalyst could be used successfully for the nitrous acid generation.

show abstract

An electrochemical immunosensor for brain natriuretic peptide prepared with screen-printed carbon electrodes nanostructured with gold nanoparticles grafted through aryl diazonium salt chemistry

Cited by 58 publications

References 20 publications

Carbon/Inorganic Hybrid Nanoarchitectures as Carriers for Signaling Elements in Electrochemical Immunosensors: First Biosensor for the Determination of the Inflammatory and Metastatic Processes Biomarker RANK‐ligand

Carbon/Inorganic Hybrid Nanoarchitectures as Carriers for Signaling Elements in Electrochemical Immunosensors: First Biosensor for the Determination of the Inflammatory and Metastatic Processes Biomarker RANK‐ligand

Manipulating nanoscale structure to control functionality in printed organic photovoltaic, transistor and bioelectronic devices

In-situ Nitrous Acid Generation over Silica Imidazole Catalyst for Dyes Production

Contact Info

Product

Resources

About