An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffold

Arenas, Christian B.; Sánchez‐Tirado, Esther; Ojeda, Irene; Gómez‐Suárez, Cristina; González‐Cortés, Araceli; Villalonga, Reynaldo; Yáñez‐Sedeño, Paloma; Pingarrón, José M.

doi:10.1016/j.snb.2015.09.055

Cited by 25 publications

(8 citation statements)

References 19 publications

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“…As can be seen in Table2, Arenas and co-workers developed an electrochemical immunosensor for adiponectin using reduced graphene oxide−carboxylmethylcellulose hybrids as electrode scaffolds. The LOD value is higher than the proposed GPbased immunosensor 43. Moreover, the modification processes take a little longer time compared to our study.…”

contrasting

confidence: 55%

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Özcan

Sezgintürk

2021

ACS Biomater. Sci. Eng.

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This study presents a disposable, novel, and sensitive biosensing system to determine adiponectin, an obesity biomarker, in real human serum. The graphite paper (GP) working electrode is a new material for impedimetric biosensors. In the literature, there is no study in which this electrode is used in impedance-based biosensors for adiponectin detection. Sensitive and useful techniques, such as electrochemical impedance spectroscopy and cyclic voltammetry, were utilized for investigation of the modification of the GP electrode surface and optimization and characterization of the constructed biosensor. The single frequency impedance technique was used to study the interactions between antiadiponectin and adiponectin. The morphology of the electrode surface for each immobilization step was examined with scanning electron microscopy. All experimental parameters were optimized to fabricate a rapid and sensitive biosensing system. The designed biosensor presents excellent performance with a wide detection range (0.05–25 pg mL–1) and a low limit of detection (0.0033 pg mL–1) for adiponectin determination. Also, it has been demonstrated that the biosensor sensitively allows for the detection of adiponectin in human serum. The affinity of the designed immunosensor toward other proteins and components was examined in the presence of the target protein (adiponectin), leptin (100 pg mL–1), creatine kinase (50 pg mL–1), parathyroid hormone (50 pg mL–1), and d-glucose (0.5 M). The selectivity of the adiponectin biosensor resulted in high capacity to neglect the interference effect. The constructed biosensor showed good linearity, long-term storage life (10 weeks), high reusability (18 times regenerability), and high ability to detect adiponectin concentrations at picogram levels.

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confidence: 55%

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Özcan

Sezgintürk

2021

ACS Biomater. Sci. Eng.

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“…The increase of current was proportional to the HIV-p24 concentration between 0.5 pg mL −1 and 8.5 ng mL −1 , with a LoD of 0.15 pg mL −1 . At last, Arenas, Sánchez-Tirado et al [ 101 ] presented the detection of adiponectin (APN), an hormone involved in glucose and lipid metabolism with a central role in the regulation of insulinoresistance, with a sandwich-type assay involving a metal complexes-based polymer (Mix & Go™) for immobilization of the anti-APN capture antibody, and HRP-anti-APN as signaling antibody ( Figure 17 C). Using amperometry at −200 mV and the H 2 O 2 /HQ redox system in solution, they obtained a linear calibration within 0.5–10.0 μg mL −1 ; the LoD was 60 ng mL −1 .…”

Section: Discussionmentioning

confidence: 99%

“…Reprinted from [ 99 ], Copyright 2013, with permission from Elsevier; ( B ) Typical DPVs corresponding to MMP-2 concentrations from a to j (0, 0.0005, 0.005, 0.02, 0.05, 0.25, 1.0, 5.0, 10.0 and 50.0 ng mL −1 ) in PBS + 4 mM H 2 O 2 , and corresponding calibration curve; ( C ) The different steps involved in the construction of the adiponectin (APN) immunosensor using a metallocomplex polymer (CMC) and RGO on a screen-printed carbon electrode; ( D ) Corresponding calibration plot. Reprinted from [ 101 ], Copyright 2016, with permission from Elsevier.…”

Section: Figurementioning

confidence: 99%

Recent Advances in Electrochemical Immunosensors

Piro

Reisberg

2017

Sensors

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Immunosensors have experienced a very significant growth in recent years, driven by the need for fast, sensitive, portable and easy-to-use devices to detect biomarkers for clinical diagnosis or to monitor organic pollutants in natural or industrial environments. Advances in the field of signal amplification using enzymatic reactions, nanomaterials such as carbon nanotubes, graphene and graphene derivatives, metallic nanoparticles (gold, silver, various oxides or metal complexes), or magnetic beads show how it is possible to improve collection, binding or transduction performances and reach the requirements for realistic clinical diagnostic or environmental control. This review presents these most recent advances; it focuses first on classical electrode substrates, then moves to carbon-based nanostructured ones including carbon nanotubes, graphene and other carbon materials, metal or metal-oxide nanoparticles, magnetic nanoparticles, dendrimers and, to finish, explore the use of ionic liquids. Analytical performances are systematically covered and compared, depending on the detection principle, but also from a chronological perspective, from 2012 to 2016 and early 2017.

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“…Arenas et al [ 30 ] reported a sandwich-type immunosensor for the determination of APN by immobilizing the CAb at CMC–rGO/SPCEs in a stable and oriented way through the metal complexes-based polymer Mix&Go TM . In addition, Btn-DAb + Strep-HRP and amperometry in the presence of H 2 O 2 /HQ were used, achieving an LOD of 61 ng mL −1 .…”

Section: Bioelectroanalytical Methods For the Determination Of Infmentioning

confidence: 99%

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The multifaceted key roles of cytokines in immunity and inflammatory processes have led to a high clinical interest for the determination of these biomolecules to be used as a tool in the diagnosis, prognosis, monitoring and treatment of several diseases of great current relevance (autoimmune, neurodegenerative, cardiac, viral and cancer diseases, hypercholesterolemia and diabetes). Therefore, the rapid and accurate determination of cytokine biomarkers in body fluids, cells and tissues has attracted considerable attention. However, many currently available techniques used for this purpose, although sensitive and selective, require expensive equipment and advanced human skills and do not meet the demands of today’s clinic in terms of test time, simplicity and point-of-care applicability. In the course of ongoing pursuit of new analytical methodologies, electrochemical biosensing is steadily gaining ground as a strategy suitable to develop simple, low-cost methods, with the ability for multiplexed and multiomics determinations in a short time and requiring a small amount of sample. This review article puts forward electrochemical biosensing methods reported in the last five years for the determination of cytokines, summarizes recent developments and trends through a comprehensive discussion of selected strategies, and highlights the challenges to solve in this field. Considering the key role demonstrated in the last years by different materials (with nano or micrometric size and with or without magnetic properties), in the design of analytical performance-enhanced electrochemical biosensing strategies, special attention is paid to the methods exploiting these approaches.

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An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffold

Cited by 25 publications

References 19 publications

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Recent Advances in Electrochemical Immunosensors

Revisiting Electrochemical Biosensing in the 21st Century Society for Inflammatory Cytokines Involved in Autoimmune, Neurodegenerative, Cardiac, Viral and Cancer Diseases

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