An electrochemical genosensing platform for the relative quantification of the circulating long noncoding RNA CCAT1 to aid in the diagnosis of colorectal cancer

Sánchez‐Salcedo, Raquel; Miranda‐Castro, Rebeca; de‐los‐Santos‐Álvarez, Noemí; Fernández-Martínez, Daniel; García-Flórez, Luis  Joaquín; Lobo-Castañón, Marı́a Jesús

doi:10.1016/j.snb.2022.132940

Cited by 7 publications

(2 citation statements)

References 31 publications

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“…This opens the opportunity of developing liquid biopsy tests based on the detection of CCAT1 for a slightly invasive screening or diagnosis of CRC. 28 Numerous studies stated the altered expression of certain lncRNAs, including CCAT1, already in precancerous adenomas. [29][30][31] Our results agreed with those of Ghafari et al, 2022, who reported that the expression levels of CCAT1 were dramatically increased, with 4.54 times more elevated levels in the blood of CRC cases contrasted to the levels seen in the controls.…”

Section: Discussionmentioning

confidence: 99%

Assessment of long noncoding RNA CCAT1 using real time-polymerase chain reaction in colorectal cancer patients

Refae

2024

EJI

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Colorectal cancer (CRC) is linked to high mortality, mainly when discovered in its advanced stages. Several studies have pointed to the role of epigenetic factors in CRC and other cancers. Long non-coding RNAs (lncRNAs) are involved in the initiation, progression, metastasis, and modulation of the response to chemotherapeutic modalities of CRC as vital contributors to epigenetic mechanisms. Colon cancer-associated transcript-1 (CCAT1) is one of the lncRNAs that have been dysregulated in serum samples, providing a non-invasive route for diagnosing CRC patients. This study aimed to determine the role of CCAT1 expression as diagnostic and prognostic markers. We tested the associations of CCAT1 expression with serum carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9). The study included three groups: 41 patients with colorectal cancer, 39 patients with precancerous benign colorectal diseases, and 20 normal control individuals. CEA and CA 19-9 were measured by an immunoassay automated system. The expression level of CCAT1 was assessed by a real-time polymerase chain reaction. There was a statistically significant elevation of serum CEA levels in patients with CRC compared to patients with precancerous benign colorectal diseases. Furthermore, there was no statistically significant difference in serum CA 19-9 levels between all groups (p = 0.102). Interestingly, CCAT1 expression was significantly upregulated in the blood of CRC patients compared to the precancerous benign colorectal diseases group (p = 0.009) and the control group (p<0.001). Also, expression of CCAT1 was significantly elevated in patients with precancerous benign colorectal diseases compared to the control group (p=0.004). In conclusion, measuring the expression level of CCAT1 is more advised than assessment of CEA and CA 19-9 for the early diagnosis and prognosis of colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

Assessment of long noncoding RNA CCAT1 using real time-polymerase chain reaction in colorectal cancer patients

Refae

2024

EJI

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“…These devices are highly desirable for their ability to provide sequence-specific information in a faster, simpler, and less costly manner than conventional assays such as PCR or RT-PCR. Genosensors find application across a diverse array of fields, including but not limited to the following: neurodegenerative diseases [19,20]; cancerous pathologies encompassing breast cancer [21][22][23], cervical cancer [24,25], gastric cancer [26,27] and colorectal cancer [28]; inherited diseases like coronary artery diseases [29,30], sickle cell anemia [31] and thalassemia [32,33]; and the detection of infectious pathogens (SARS-CoV-2 [34,35], Influenza A virus [36,37], Haemophilus influenza [38], and Mycobacterium tuberculosis [39,40]).…”

Section: Introductionmentioning

confidence: 99%

Designing a Simple Electrochemical Genosensor for the Detection of Urinary PCA3, a Prostate Cancer Biomarker

Mokni,

Tlili,

Khalij

et al. 2024

Micromachines

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This study investigates the feasibility of a simple electrochemical detection of Prostate Cancer Antigen 3 (PCA3) fragments extracted from patients’ urine, using a thiolated single-strand DNA probe immobilized on a gold surface without using a redox probe. To enhance the PCA3 recognition process, we conducted a comparative analysis of the hybridization location using two thiolated DNA probes: Probe 1 targets the first 40 bases, while Probe 2 targets the fragment from bases 47 to 86. Hybridization with PCA3 followed, using square wave voltammetry. The limit of detection of the designed genosenors were of the order of (2.2 ng/mL), and (1.6 ng/mL) for Probes 1 and 2, respectively, and the subsequent sensitivities were of the order of (0.09 ± 0.01) µA−1 · µg−1 · mL and (0.10 ± 0.01) µA−1 · µg−1 · mL. Specificity tests were then conducted with the sensor functionalized with Probe 2, as it presents better analytical performances. The electrochemical results indicate that the designed sensor can clearly discriminate a complementary target from a non-complementary one. A further modeling of the calibration curves with the Power Law/Hill model indicates that the dissociation constant increases by one order of magnitude, confirming the ability of the designed sensor to perfectly discriminate complementary targets from non-complementary ones.

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Electrochemical sensors: basic principles, engineering, and state of the art

Saputra¹

2023

Monatsh Chem

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An electrochemical genosensing platform for the relative quantification of the circulating long noncoding RNA CCAT1 to aid in the diagnosis of colorectal cancer

Cited by 7 publications

References 31 publications

Assessment of long noncoding RNA CCAT1 using real time-polymerase chain reaction in colorectal cancer patients

Assessment of long noncoding RNA CCAT1 using real time-polymerase chain reaction in colorectal cancer patients

Designing a Simple Electrochemical Genosensor for the Detection of Urinary PCA3, a Prostate Cancer Biomarker

Electrochemical sensors: basic principles, engineering, and state of the art

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