Abstract:An electrochemical microbiosensor for DNA has been fabricated based on new acrylic microspheres modified with reactive N-acryloxysuccinimide (NAS) functional groups. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesized in an emulsion form with a simple one-step photopolymerization technique. Aminated DNA probe was attached to the succinimde functional group of the acrylic microspheres via covalent bonding. The hybridization of the immobilized DNA probe with the complementary DN… Show more
“…Another electrochemical sensing utilized hydrophobic poly(nBA-co-NHSA) microspheres, which were functionalized with ssDNA, during differential pulse voltametry using anthraquninone-2-sulfonic acid monohydrate sodium salt (AQMS) as the electroactive hybridization label. 507 6.3.4. Peptide/Protein Polymer Conjugates.…”
“…Another electrochemical sensing utilized hydrophobic poly(nBA-co-NHSA) microspheres, which were functionalized with ssDNA, during differential pulse voltametry using anthraquninone-2-sulfonic acid monohydrate sodium salt (AQMS) as the electroactive hybridization label. 507 6.3.4. Peptide/Protein Polymer Conjugates.…”
“…This hybridized label-free DNA biosensor reduces the R CT signal from 0.2 to 2 µM, reducing the probe DNA capacity as a result of a higher hybridization process rate on the electrode surfaces, concurrent with probe DNA addition. The DNA hybridization rate depends on the total amount of immobilized probe DNA in the immobilization matrix [36]. The probe DNA surface density is vital, especially in a detection mechanism that relies on the intrinsic charge of the DNA phosphate backbone.…”
Section: Optimization Of Label-free Dna Biosensor Responsementioning
A novel label-free electrochemical DNA biosensor was constructed for the determination of Escherichia coli bacteria in environmental water samples. The aminated DNA probe was immobilized onto hollow silica microspheres (HSMs) functionalized with 3-aminopropyltriethoxysilane and deposited onto a screen-printed electrode (SPE) carbon paste with supported gold nanoparticles (AuNPs). The biosensor was optimized for higher specificity and sensitivity. The label-free E. coli DNA biosensor exhibited a dynamic linear response range of 1 × 10−10 µM to 1 × 10−5 µM (R2 = 0.982), with a limit of detection at 1.95 × 10−15 µM, without a redox mediator. The sensitivity of the developed DNA biosensor was comparable to the non-complementary and single-base mismatched DNA. The DNA biosensor demonstrated a stable response up to 21 days of storage at 4 ℃ and pH 7. The DNA biosensor response was regenerable over three successive regeneration and rehybridization cycles.
“…Acrylic microspheres were non-conducting materials, and thus the addition of gold nanoparticles could enhance the electron transfer from the mediator (K 2 Fe(CN) 6 ) to SPE surface (Ulianas et al 2012). The experiment showed a gradual increase of biosensor response from 0.02 to 0.04 mg AuNPs but a sudden increase with almost two-fold current by 1 mg particle up to 0.05 mg were observed.…”
Section: Characterisation Of Modified Hrp/msp-nas/aunps/spementioning
The sensitive and rapid detection of hydrogen peroxide is very important in the areas of clinical and environmental analyses. A sensitive and selective Horseradish peroxidase (HRP)-hydrogen peroxide (H 2 O 2 ) biosensor was developed based on acrylic microspheres. Hydrophobic poly (n-butyl acrylate-N-acryloxysuccinimide) [poly(nBA-NAS)] microspheres were synthesized using photopolymerization in an emulsion to form an enzyme immobilization matrix. The HRP enzyme was covalently immobilized onto the acrylic microspheres via the succinimide functionality. Field emission scanning electron microscope (FESEM) has been utilized to characterize the screen-printed carbon paste electrode (SPE) constructed from enzyme conjugated acrylic microspheres and gold nanoparticles (AuNPs) composite (HRP/nBA-NAS/AuNPs/SPE). Differential pulse voltammetry was used to assess the biosensor performance. The linear response range of the hydrogen peroxide biosensor obtained was from 1.0 × 10 -2 to 1.0 × 10 -10 M (R 2 = 0.99) with the limit of detection (LOD) approximately at 1.0 × 10 -10 M. This is an improvement over many hydrogen peroxide biosensors reported so far. Such improvement may be attributed to the large surface area provided by the acrylic microspheres as a matrix for immobilization of the HRP enzyme.
ABSTRAKPengesanan hidrogen peroksida yang sensitif dan cepat adalah sangat penting dalam bidang analisis persekitaran dan klinikal. Biosensor hidrogen peroksida (H 2 O 2 )-peroksidase lobak putih (HRP) yang sensitif dan memilih telah dibangunkan berasaskan mikrosfera akrilik. Mikrosfera hidrofobik poli (n-butil akrilat-N-akriloksuksinimida) [poli(nBA-NAS)] disintesis dengan menggunakan proses fotopempolimeran dalam bentuk emulsi dan ia bertindak sebagai matriks pemegun enzim. Enzim HRP dipegunkan secara kovalen pada mikrosfera akrilik melalui pengfungsian suksinimida. Mikroskop elektron imbasan pancaran medan (FESEM) telah digunakan bagi mencirikan elektrod karbon permukaan bercetak (SPE) yang dibina daripada komposit mikrosfera akrilik berkonjugat enzim dan nanosfera emas (AuNPs) (HRP/nBA-NAS/AuNPs/SPE). Voltametri denyutan pembezaan digunakan untuk penilaian prestasi biosensor. Julat keupayaan linear bagi biosensor hidrogen peroksida diperoleh daripada 1.0 × 10 -2 hingga 1.0 × 10 -10 M (R 2 = 0.99) dengan had pengesanan (LOD) ditemui pada 1 × 10 -10 M. Ini merupakan penambahbaikan berbanding biosensor hidrogen peroksida yang telah terlebih dahulu dilaporkan. Penambahbaikan ini mungkin ditentukan oleh luas permukaan yang besar yang disediakan oleh mikrosfera akrilik sebagai tapak pemegunan enzim HRP. Kata kunci: Biosensor; hidrogen peroksida; mikrosfera akrilik; peroksidase lobak putih
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