An electrochemical biosensor for the assay of alpha-fetoprotein-L3 with practical applications

Li, Jinxin; Gao, Tao; Gu, Shiyu; Jun, Zhi; Yang, Jie; Li, Genxi

doi:10.1016/j.bios.2016.08.071

Cited by 64 publications

(25 citation statements)

References 27 publications

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“…Combining detection of AFP with new diagnostic markers increases the sensitivity and specificity to diagnose HCC. For instance, combination of AFP and lens culinaris lectin detection resulted in increased specificity and sensitivity in diagnosing HCC [ 8 , 9 ]. With the progress of clinical laboratory techniques, the new liver biomarkers have displayed important roles in the diagnosis of liver cancer.…”

Section: Significance Of Alpha-fetoprotein Expression In Hepatocelmentioning

confidence: 99%

Alpha-Fetoprotein and Hepatocellular Carcinoma Immunity

Wang

2018

Canadian Journal of Gastroenterology and Hepatology

109

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Hepatocarcinoma is one of the most prevalent gastroenterological cancers in the world with less effective therapy. As an oncofetal antigen and diagnostic marker for liver cancer, alpha-fetoprotein (AFP) possesses a variety of biological functions. Except for its diagnosis in liver cancer, AFP has become a target for liver cancer immunotherapy. Although the immunogenicity of AFP is weak and it could induce the immune escapes through inhibiting the function of dendritic cells, natural killer cells, and T lymphocytes, AFP has attracted more attention in liver cancer immunotherapy. By in vitro modification, the immunogenicity and immune response of AFP could be enhanced. AFP-modified immune cell vaccine or peptide vaccine has displayed the specific antitumor immunity against AFP-positive tumor cells and laid a better foundation for the immunotherapy of liver cancer.

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Section: Significance Of Alpha-fetoprotein Expression In Hepatocelmentioning

confidence: 99%

Alpha-Fetoprotein and Hepatocellular Carcinoma Immunity

Wang

2018

Canadian Journal of Gastroenterology and Hepatology

109

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“…Because of the lack of early diagnostic biomarkers with high specificity and sensitivity, patients with HCC usually fail to receive timely treatment 2 . The classical biomarkers for clinical diagnosis include α-fetoprotein (AFP) 3 , α-fetoprotein-L3 (AFP-L3) 4 , and des-carboxy-prothrombin (DCP) 5 . However, these biomarkers lead to some false-positive and false-negative results in HCC diagnosis.…”

Section: Introductionmentioning

confidence: 99%

circRNA_104075 stimulates YAP-dependent tumorigenesis through the regulation of HNF4a and may serve as a diagnostic marker in hepatocellular carcinoma

et al. 2018

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Some types of circular RNA (circRNA) are aberrantly expressed in human diseases including hepatocellular carcinoma (HCC). However, its regulation mechanism and diagnostic roles are largely unknown. Here, we identified that circRNA_104075 (circ_104075) was highly expressed in HCC tissues, cell lines and serum. Mechanistically, HNF4a bound to the −1409 to −1401 region of the circ_104075 promoter to stimulate the expression of circ_104075. Moreover, circ_104075 acted as a ceRNA to upregulate YAP expression by absorbing miR-582-3p. Interestingly, an N6-methyladenosine (m6A) motif was identified in the 353–357 region of YAP 3′UTR, and this m6A modification was essential for the interaction between miR-582-3p and YAP 3′UTR. Further, the diagnostic performance of circ_104075 was evaluated. The area under the receiver operating characteristic (AUC-ROC) for circ_104075 was 0.973 with a sensitivity of 96.0% and a specificity of 98.3%. Collectively, we determined that circ_104075 was highly expressed in HCC and elucidated its upstream and downstream regulatory mechanisms. circ_104075 additionally has the potential to serve as a new diagnostic biomarker in HCC. Targeting circ_104075 may provide new strategies in HCC diagnosis and therapy.

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“…For detecting AFP-L3, several methods are available, such as the affinity adsorption assay, 11 immune electrophoresis, 12 , 13 enzyme-linked immunoassay, the electrochemical technique, 14 affinity chromatography, and affinity imprinting. However, all of these methods have some disadvantages, including being time-consuming, complex to perform, having low sensitivity, requiring a large sample size, and requiring sophisticated instruments.…”

Section: Introductionmentioning

confidence: 99%

Analysis of serum alpha-fetoprotein (AFP) and AFP-L3 levels by protein microarray

Haidong

Liu

et al. 2018

J Int Med Res

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ObjectivesThis study aimed to examine a simple, effective, time-saving, and low-cost protein microarray method for detecting serum alpha-fetoprotein (AFP) and AFP-L3 levels.MethodsSerum samples from patients with hepatocellular carcinoma (HCC) (n = 33) and control subjects (n = 39) were collected and evaluated for the presence of AFP using a novel protein microarray. Glycoprotein (including AFP-L3) was enriched from crude samples by a Hotgen Biotech glycosyl capture spin column and then detected by protein microarray. An electrochemiluminescence immunoassay (ECLIA) was used to validate the measured values.ResultsNeither AFP levels lower than 20 ng/mL in the HCC group nor AFP levels higher than 20 ng/mL in the control group were found when tested by the ECLIA and protein microarray. The kappa test showed good consistency in the diagnostic performance of measuring serum AFP levels and the percentage of AFP-L3 in total AFP by the ECLIA and protein microarray. Protein microarray had advantages of smaller sample size required, low cost, and convenience compared with the ECLIA.ConclusionThe protein microarray assay that was developed in the present study shows potential as an economic and convenient technique for detecting AFP and AFP-L3 levels in serum samples from patients with HCC.

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An electrochemical biosensor for the assay of alpha-fetoprotein-L3 with practical applications

Cited by 64 publications

References 27 publications

Alpha-Fetoprotein and Hepatocellular Carcinoma Immunity

Alpha-Fetoprotein and Hepatocellular Carcinoma Immunity

circRNA_104075 stimulates YAP-dependent tumorigenesis through the regulation of HNF4a and may serve as a diagnostic marker in hepatocellular carcinoma

Analysis of serum alpha-fetoprotein (AFP) and AFP-L3 levels by protein microarray

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