An electrochemical aptasensor based on AuPt alloy nanoparticles for ultrasensitive detection of amyloid-β oligomers

Liu, Yibiao; Xu, Qing; Zhang, Yina; Ren, Bingyu; Huang, Liumei; Cai, Hong; Xu, Tailin; Liu, Qiong; Zhang, Xueji

doi:10.1016/j.talanta.2021.122360

Cited by 35 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The LOD is calculated by three times the SD of the blank according to our previous report. ( Yibiao, Qing et al, 2021 ) As a control, a typical ELISA has been used to validate our method. As shown in Supplementary Figure S8 , the ELISA calibration curve was constructed by plotting the optical density of Aβ42 at 450 nm (TMB as substrate).…”

Section: Resultsmentioning

confidence: 99%

Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein

Zhou

et al. 2021

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Amyloid-β protein (Aβ) is an important biomarker and plays a key role in the early stage of Alzheimer’s disease (AD). Here, an ultrasensitive photoelectrochemical (PEC) sensor based on ZnO@polydopamine/Au nanocomposites was constructed for quantitative detection of Aβ. In this sensing system, the ZnO nanorod array decorated with PDA films and gold nanoparticles (Au NPs) have excellent visible-light activity. The PDA film was used as a sensitizer for charge separation, and it also was used for antibody binding. Moreover, Au NPs were loaded on the surface of PDA film by in situ deposition, which further improved the charge transfer efficiency and the PEC activity in visible light due to the localized surface plasmon resonance effect of Au NPs. Therefore, in ZnO@polydopamine/Au nanocomposites, a significantly enhanced photocurrent response was obtained on this photoelectrode, which provides a good and reliable signal for early detection of AD. Under the optimized conditions, the PEC immunosensor displayed a wide linear range from 1 pg/mL to 100 ng/mL and a low detection limit of 0.26 pg/mL. In addition, this PEC immunosensor also presented good selectivity, stability, and reproducibility. This work may provide a promising point-of-care testing method toward advanced PEC immunoassays for AD biomarkers.

show abstract

Section: Resultsmentioning

confidence: 99%

Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein

Zhou

et al. 2021

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…This increases the sensitivity and stability of the (bio)sensors developed, catalyzes electrochemical reactions applied for the detection, enhances the electron transfer at the electrode, and assures the immobilization of different types of labels, which can highly improve the performance of the device [ 13 , 89 ]. Thus, either graphene combined with metal nanoparticles [ 89 ], polymers combined with metallic foams [ 90 ] or particles [ 91 ], or composites based on noble metal particles or alloys [ 92 ], or with MOFs [ 93 ], were deposited on the electrode for increased sensitivity.…”

Section: Biomarkers Involved In Inflammatory Diseasesmentioning

confidence: 99%

“…A poly(curcumin-Ni foam)-based sensor was fabricated and successfully applied for the quantification of amyloid β oligomer (AβO), a circulating biomarker applied for early diagnosis of Alzheimer’s disease [ 90 ]. For the same biomarker, another detection strategy was proposed using superhydrophobic carbon fiber paper and AuPt-NPs to immobilize the aptamer strands specific for AβO and indirectly minimize the nonspecific adsorption effect due to its hydrophobic character [ 92 ]. After the functionalization of GCEs with cerium oxide nanofiber electrospun, embedded in a Nafion layer, the composite film generated at the electrode proved to be permeable in a selective way for the TNF-α biomarker, with fast electron transfer and excellent analytical performance in serum [ 96 ].…”

Section: Biomarkers Involved In Inflammatory Diseasesmentioning

confidence: 99%

“…The use of aptamers as a biomimetic element for the selective detection of biomarkers involved in neuro-zone diseases has also been reported. Thus, a large number of AβO-specific aptamers were immobilized on a composite layer based on CNF and AuPt alloy that allowed the elaboration of a high-performance electrochemical aptasensor for AβO [ 92 ]. The suitability of aptamers for functionalization allows the addition on their chain of some compounds or composites with a role in detection.…”

Section: Biomarkers Involved In Inflammatory Diseasesmentioning

confidence: 99%

See 1 more Smart Citation

Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

et al. 2022

View full text Add to dashboard Cite

Recent years have shown that the diagnosis and monitoring of biomarkers involved in inflammatory-associated medical conditions such as cancer, neurological disorders, viral infections, or daily physical activities offer real benefits in increasing the quality of medical care and patient life quality. In this context, the use of integrated and portable platforms as point-of-care testing devices for biomedical analysis to enable early disease diagnosis and monitoring, which can be successfully used even at the patient’s bed, is an emergency nowadays. The development of low-cost, miniaturized, and portable, user-friendly devices that provide an answer in a timely manner, such as electrochemical sensors, is relevant for the elaboration of point-of-care testing devices. This review focuses on the recent progress in bioanalysis of both specific biomarkers and inflammatory-associated biomarkers present in several diseases like neoplasia, severe neurological disorders, viral infections, and usual physical activity and provides an overview of the state of the art over the most recent electrochemical (bio)sensors for the detection of inflammation-related biomarkers. Future perspectives of point-of-care testing to improve healthcare management are also discussed. Graphical abstract

show abstract

“…Although these traditional immunological methods provide invaluable insights for the analysis of VEGF165, they are labor-intensive and time-consuming and require cumbersome operating protocols, which limit their application in real-time clinical diagnosis. In recent years, aptamer-based biosensors have gained strong clinical application prospects for the detection of disease-specific biomarkers ( Zhang et al, 2018 ; Gao S. et al, 2020 ; Chinnappan et al, 2021 ; Gao et al, 2021 ; Liu et al, 2021 ; Xu et al, 2021 ). An aptamer, defined as a chemical antibody, is a functional single-stranded DNA or RNA oligonucleotide that can bind to a target with high affinity and specificity ( Ellington and Szostak, 1990 ; Tuerk and Gold, 1990 ).…”

Section: Introductionmentioning

confidence: 99%

A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165

Zheng

Gao

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

VEGF165, a regulator of angiogenesis, has been widely used as a serum biomarker for a number of human diseases, including cancer, rheumatoid arthritis, bronchial asthma, and diabetic eye disease. The rapid, accurate, and convenient detection of VEGF165 is a crucial step in effective healthcare monitoring, disease diagnosis, and prognosis assessment. In this study, a fluorescent aptasensor based on an assembled G-quadruplex and the signal molecule ThT was developed for VEGF165 detection. First, G-rich DNA fragments were assembled at both ends of the anti-VEGF165 aptamer, and the B-DNA form was converted into a G-quadruplex structure aptamer (G4-Apt). Then, ThT was introduced, and the G-quadruplex significantly enhanced the fluorescence intensity of the bound ThT. When VEGF165 was present, the higher affinity of the aptamer to the target protein allowed the G4-Apt/VEGF165 complex to form and release ThT, which emitted only weak fluorescence in the free state. Therefore, the aptasensor exhibited a good linear detection window from 1.56 to 25 nM VEGF165, with a limit of detection of 0.138 nM. In addition, the aptasensor was applied to detect VEGF165 in clinical serum samples, showing good accuracy, reproducibility, and stability. These results indicate that our developed fluorescent aptasensor can potentially be a reliable, convenient, and cost-effective approach for the sensitive, specific, and rapid detection of the VEGF165 biomarker.

show abstract

An electrochemical aptasensor based on AuPt alloy nanoparticles for ultrasensitive detection of amyloid-β oligomers

Cited by 35 publications

References 39 publications

Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein

Bioinspired Synthesis of ZnO@polydopamine/Au for Label-Free Photoelectrochemical Immunoassay of Amyloid-β Protein

Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165

Contact Info

Product

Resources

About