An electrochemical aptasensor based on DNA-AuNPs-HRP nanoprobes and exonuclease-assisted signal amplification for detection of aflatoxin B1

Hui, Yuanyuan; Wang, Bini; Ren, Rong; Zhao, Aiqing; Zhang, Fuxin; Song, Shuanghong; He, Yaping

doi:10.1016/j.foodcont.2019.106902

Cited by 54 publications

(22 citation statements)

References 37 publications

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“…Traditional amplification strategies include labeling amplification, where a single nanomaterial-based tag incorporates numerous signal-generation elements, and target amplification, where a single target generates many signaling molecules through a multitude of analyte-recognition events [ 120 ]. Numerous aptasensing studies focused on food contaminants have employed the first strategy, based on nanomaterials, such as carbon nanomaterials (CNs) [ 28 , 96 , 124 , 125 ] and metal nanoparticles [ 125 , 126 , 127 ], or enzyme labeling [ 69 , 79 ]. The second signal amplification strategy aims to increase the signal/target concentration ratio, as in the case of nuclease-based target recycling [ 127 ].…”

Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

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Food safety monitoring assays based on synthetic recognition structures such as aptamers are receiving considerable attention due to their remarkable advantages in terms of their ability to bind to a wide range of target analytes, strong binding affinity, facile manufacturing, and cost-effectiveness. Although aptasensors for food monitoring are still in the development stage, the use of an electrochemical detection route, combined with the wide range of materials available as transducers and the proper immobilization strategy of the aptamer at the transducer surface, can lead to powerful analytical tools. In such a context, employing aryldiazonium salts for the surface derivatization of transducer electrodes serves as a simple, versatile and robust strategy to fine-tune the interface properties and to facilitate the convenient anchoring and stability of the aptamer. By summarizing the most important results disclosed in the last years, this article provides a comprehensive review that emphasizes the contribution of aryldiazonium chemistry in developing electrochemical aptasensors for food safety monitoring.

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Section: Strategies In Electrochemical Aptasensor Design: Aptamer Immobilization and Electrochemical Signal Generationmentioning

confidence: 99%

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Raicopol

Pilan

2021

Materials

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“…An extremely low LOD of 1.37 × 10 −6 µg kg −1 was obtained with a wide linear range of 1 × 10 −5 to 10 µg kg −1 for ZEN detection [ 122 ]. MCH is also used to ensure the linear structure of probe DNA strands in some aptasensors [ 123 ]. Aptasensors based on DPV often require a signal amplifier in addition to surface modification, as shown in Table 5 .…”

Section: Conventional and Advanced Analytical Technologiesmentioning

confidence: 99%

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Ong

Pike

Tan

2021

Foods

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The presence of mycotoxins in foodstuffs and feedstuffs is a serious concern for human health. The detection of mycotoxins is therefore necessary as a preventive action to avoid the harmful contamination of foodstuffs and animal feed. In comparison with the considerable expense of treating contaminated foodstuffs, early detection is a cost-effective way to ensure food safety. The high affinity of bio-recognition molecules to mycotoxins has led to the development of affinity columns for sample pre-treatment and the development of biosensors for the quantitative analysis of mycotoxins. Aptamers are a very attractive class of biological receptors that are currently in great demand for the development of new biosensors. In this review, the improvement in the materials and methodology, and the working principles and performance of both conventional and recently developed methods are discussed. The key features and applications of the fundamental recognition elements, such as antibodies and aptamers are addressed. Recent advances in aptasensors that are based on different electrochemical (EC) transducers are reviewed in detail, especially from the perspective of the diagnostic mechanism; in addition, a brief introduction of some commercially available mycotoxin detection kits is provided.

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“…After adding the DNA-AuNPs-HRP nanoprobes, it will bind to cDNA Functionalized AuNPs that can significantly enhance and amplify electrochemical signals can be applied to manufacture a variety of electrochemical sensing devices with high sensitivity, good selectivity, high reliability and low cost. Hui et al reported a electrochemical aptasensor for the detection of AFB 1 [155] (Figure 11a). The complementary DNA (cDNA) of AFB 1 Apt was immobilized on the surface of the AuNPs modified electrode.…”

Section: Electrochemical Sensormentioning

confidence: 99%

“…(a) Electrochemical aptasensor based on DNA-AuNPs-HRP nanoprobes for detection of AFB 1 . Reproduced with permission from[155]. Copyright Food Control, 2020.…”

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confidence: 99%

Noble Metal Nanostructured Materials for Chemical and Biosensing Systems

et al. 2020

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Nanomaterials with unique physical and chemical properties have attracted extensive attention of scientific research and will play an increasingly important role in the future development of science and technology. With the gradual deepening of research, noble metal nanomaterials have been applied in the fields of new energy materials, photoelectric information storage, and nano-enhanced catalysis due to their unique optical, electrical and catalytic properties. Nanostructured materials formed by noble metal elements (Au, Ag, etc.) exhibit remarkable photoelectric properties, good stability and low biotoxicity, which received extensive attention in chemical and biological sensing field and achieved significant research progress. In this paper, the research on the synthesis, modification and sensing application of the existing noble metal nanomaterials is reviewed in detail, which provides a theoretical guidance for further research on the functional properties of such nanostructured materials and their applications of other nanofields.

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An electrochemical aptasensor based on DNA-AuNPs-HRP nanoprobes and exonuclease-assisted signal amplification for detection of aflatoxin B1

Cited by 54 publications

References 37 publications

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

The Role of Aryldiazonium Chemistry in Designing Electrochemical Aptasensors for the Detection of Food Contaminants

Recent Advances in Conventional Methods and Electrochemical Aptasensors for Mycotoxin Detection

Noble Metal Nanostructured Materials for Chemical and Biosensing Systems

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