An Elastic, Biodegradable Cardiac Patch Induces Contractile Smooth Muscle and Improves Cardiac Remodeling and Function in Subacute Myocardial Infarction

Fujimoto, Kazuro; Tobita, Kimimasa; Merryman, W. David; Guan, Jianjun; Momoi, Nobuo; Stolz, Donna B.; Sacks, Michael S.; Keller, Bradley B.; Wagner, William R.

doi:10.1016/j.jacc.2007.02.050

Cited by 206 publications

(161 citation statements)

References 26 publications

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“…PEUU biodegradable scaffolds meet all of these requirements and are currently being studied in several tissue engineering applications. 28,29,32,33 Although most porous scaffold processing techniques greatly reduce the mechanical properties relative to the bulk polymer, electrospinning retains a great deal of the strength and distensibility of the bulk PEUU. 29,32 In our study, the combination of two processing techniques to obtain an ES-TIPS scaffold provides the necessary porosity for cell support and tissue ingrowth while maintaining the mechanical properties to withstand systemic circulation.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of a Tissue-Engineered Vascular Graft Combining a Biodegradable Elastomeric Scaffold and Muscle-Derived Stem Cells in a Rat Model

Nieponice

Soletti

Guan

et al. 2010

Tissue Engineering Part A

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Limited autologous vascular graft availability and poor patency rates of synthetic grafts for bypass or replacement of small-diameter arteries remain a concern in the surgical community. These limitations could potentially be improved by a tissue engineering approach. We report here our progress in the development and in vivo testing of a stem-cell-based tissue-engineered vascular graft for arterial applications. Poly(ester urethane)urea scaffolds (length ¼ 10 mm; inner diameter ¼ 1.2 mm) were created by thermally induced phase separation (TIPS). Compound scaffolds were generated by reinforcing TIPS scaffolds with an outer electrospun layer of the same biomaterial (ES-TIPS). Both TIPS and ES-TIPS scaffolds were bulk-seeded with 10Â10 6 allogeneic, LacZ-transfected, muscle-derived stem cells (MDSCs), and then placed in spinner flask culture for 48 h. Constructs were implanted as interposition grafts in the abdominal aorta of rats for 8 weeks. Angiograms and histological assessment were performed at the time of explant. Cell-seeded constructs showed a higher patency rate than the unseeded controls: 65% (ES-TIPS) and 53% (TIPS) versus 10% (acellular TIPS). TIPS scaffolds had a 50% mechanical failure rate with aneurysmal formation, whereas no dilation was observed in the hybrid scaffolds. A smooth-muscle-like layer of cells was observed near the luminal surface of the constructs that stained positive for smooth muscle a-actin and calponin. LacZþ cells were shown to be engrafted in the remodeled construct. A confluent layer of von Willebrand Factor-positive cells was observed in the lumen of MDSC-seeded constructs, whereas acellular controls showed platelet and fibrin deposition. This is the first evidence that MDSCs improve patency and contribute to the remodeling of a tissue-engineered vascular graft for arterial applications.

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Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of a Tissue-Engineered Vascular Graft Combining a Biodegradable Elastomeric Scaffold and Muscle-Derived Stem Cells in a Rat Model

Nieponice

Soletti

Guan

et al. 2010

Tissue Engineering Part A

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145

123

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“…However, the pertinence of acellular patches for cardiac function improvement has been demonstrated [14,15]. Epicardial implantation of acellular patches after myocardial injury resulted in left ventricular (LV) function improvement and limited progression of LV dilation.…”

Section: Introductionmentioning

confidence: 99%

Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization

et al. 2014

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Cardiac tissue engineering approaches can deliver large numbers of cells to the damaged myocardium and have thus increasingly been considered as a possible curative treatment to counteract the high prevalence of progressive heart failure after myocardial infarction (MI). Optimal scaffold architecture and mechanical and chemical properties, as well as immune-and bio-compatibility, need to be addressed. We demonstrated that radio-frequency plasma surface functionalized electrospun poly (e-caprolactone) (PCL) fibres provide a suitable matrix for bone-marrow-derived mesenchymal stem cell (MSC) cardiac implantation. Using a rat model of chronic MI, we showed that MSC-seeded plasma-coated PCL grafts stabilized cardiac function and attenuated dilatation. Significant relative decreases of 13% of the ejection fraction (EF) and 15% of the fractional shortening (FS) were observed in sham treated animals; respective decreases of 20% and 25% were measured 4 weeks after acellular patch implantation, whereas a steadied function was observed 4 weeks after MSC-patch implantation (relative decreases of 6% for both EF and FS).

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“…Nowadays synthetic materials have achieved high degrees of biodegradation and biocompatibility, like their natural counterparts. In fact, cardiac administration of synthetic hydrogels has proved to be effective in terms of promoting contractile phenotype smooth muscle tissue formation [46,47], preventing LV remodeling and scar expansion and improving cardiac function [48][49][50][51]. Interestingly, the timing of administration seems to be important because hydrogels assumed markedly different morphologies that determined heart remodeling.…”

Section: Hydrogelsmentioning

confidence: 99%

Heart regeneration after myocardial infarction using synthetic biomaterials

Pascual-Gil

Garbayo

Díaz-Herráez

et al. 2015

Journal of Controlled Release

121

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Abstract:Myocardial infarction causes almost 7.3 million deaths each year worldwide. However, current treatments are more palliative than curative. Presently, cell and protein therapies are considered the most promising alternative treatments. Clinical trials performed until now have demonstrated that these therapies are limited by protein short half-life and by low transplanted cell survival rate, prompting the development of novel cell and protein delivery systems able to overcome such limitations. In this review we discuss the advances made in the last 10 years in the emerging field of cardiac repair using biomaterial-based delivery systems with focus on the progress made on preclinical in vivo studies. Then, we focus in cardiac tissue engineering approaches, and how the incorporation of both cells and proteins together into biomaterials has opened new horizons in the myocardial infarction treatment. Finally, the ongoing challenges and the perspectives for future work in cardiac tissue engineering will also be discussed.

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An Elastic, Biodegradable Cardiac Patch Induces Contractile Smooth Muscle and Improves Cardiac Remodeling and Function in Subacute Myocardial Infarction

Cited by 206 publications

References 26 publications

In Vivo Assessment of a Tissue-Engineered Vascular Graft Combining a Biodegradable Elastomeric Scaffold and Muscle-Derived Stem Cells in a Rat Model

In Vivo Assessment of a Tissue-Engineered Vascular Graft Combining a Biodegradable Elastomeric Scaffold and Muscle-Derived Stem Cells in a Rat Model

Plasma-functionalized electrospun matrix for biograft development and cardiac function stabilization

Heart regeneration after myocardial infarction using synthetic biomaterials

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