An egg ist ein ei, es un huevo, est un oeuf

Solomon, Sarah E.

doi:10.1080/00071669987764

Cited by 15 publications

(5 citation statements)

References 21 publications

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“…These biomacromolecules, especially the matrix proteins, are believed to be active in controlling the morphology and selective nucleation of polymorphs of the CaCO 3 layer of the eggshell (39). We focused our attention on understanding the molecular mechanism of eggshell calcification through extraction, purification, and characterization of the matrix proteins (40). Recently, a number of proteins and peptides from the goose eggshell extract were isolated, purified, and characterized.…”

Section: Structural Characterization Of a Goose Eggshell Protein Ansmentioning

confidence: 99%

Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix

Lakshminarayanan

Kini

Valiyaveettil

2002

Proc. Natl. Acad. Sci. U.S.A.

100

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The role of proteins in biomineralization and the mechanism of eggshell formation are not well understood. We have isolated and purified the major protein, ansocalcin from goose eggshell matrix. The amino acid sequence study indicates that ansocalcin is homologous to the chicken eggshell protein, ovocleidin 17, and C-type lectins. Ansocalcin nucleates polycrystalline aggregates of calcite crystals in in vitro mineralization experiments. The polycrystalline aggregates obtained at higher concentration of ansocalcin appears to be similar to the crystals observed at the mamillary layer of the eggshell.B iomineralization is commonly referred to as the formation of biocomposites consisting of layered assembly of biomacromolecules such as proteins with well ordered calcium-rich inorganic phase (1-3). This highly complex process involves a series of molecular events such as selective recognition and deposition of calcium salts by proteins followed by the formation of a mineral phase comprising crystallites of uniform size, crystallographic orientation, and morphology (4, 5). In most cases, the minerals are formed over a biomolecular scaffold or mold resulting in composite materials (6, 7). The role of such biologically programmed composites varies from skeletal tissues to protective shells against the predators (8, 9). These highly complex, hierarchically ordered and multifunctional composites are formed under mild conditions with size ranges from nano-to centimeter scale, exhibiting unusual mechanical properties that outperform synthetic materials (10-15). Such structures appeared to originate from organized assembly of biomacromolecules such as proteins, polysaccharides or proteoglycans, and the inorganic salt (16). The organic macromolecules act as templates through self-assembly to facilitate interaction with the insoluble matrix and to induce the desired stereochemistry for the construction of organized structures (17-19). Biomacromolecules are known to be involved in controlling the nucleation, growth, size, and shape of the mineral phases (20). Often these macromolecules are functionalized with acidic groups such as carboxylic acid, sulfonate, and phosphate moieties, which allow them to be an effective metal ion chelator to interact with the inorganic matrix (21-23).Avian eggshells present a unique and interesting model for exploring the process of biomineralization, in which CaCO 3 layers are created by the selective nucleation and deposition of calcite crystals by proteins. Moreover, the active sites on the protein recognize calcium ions and induce nucleation of specific polymorph of CaCO 3 and control the morphology of mineral phase. In the case of chicken eggshells, Ϸ5 g of CaCO 3 is deposited within 22 h in an acellular medium as the egg passes through the oviduct, which makes avian eggshells one of the fastest mineralized hard tissues in biological systems (24). The structure of an avian eggshell consists of two principal external layers, namely a membrane layer that surrounds the albumin and a calcified sh...

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Section: Structural Characterization Of a Goose Eggshell Protein Ansmentioning

confidence: 99%

Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix

Lakshminarayanan

Kini

Valiyaveettil

2002

Proc. Natl. Acad. Sci. U.S.A.

100

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“…Although one family of matrix proteins corresponds to the egg white proteins:lysozyme (14.4 kDa), ovotransferrin (78 kDa), and ovalbumin (43 kDa; Refs. 5,6), another group of proteins in the eggshell is the bone matrix proteins osteopontin and sialoprotein (7). Finally, the third group of proteins that is specifically found in the uterus includes the ovocleidins and ovocalyxins.…”

mentioning

confidence: 99%

Crystal Structure of Ovocleidin-17, a Major Protein of the Calcified Gallus gallus Eggshell

Reyes‐Grajeda

Moreno

Romero

2004

Journal of Biological Chemistry

104

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Ovocleidin-17 (OC17) from Gallus gallus is one of the best candidates to control and regulate the deposition of calcium carbonate in the calcified eggshell layer. Here, the crystal structure of monomeric OC17, determined at a resolution of 1.5 Å, was refined to a crystallographic R-factor of 20.1%. This is the first protein directly involved in a non-pathological biomineralization process resolved by x-ray diffraction to date. The protein has a mixed ␣/␤ structure containing a single C-type lectinlike domain. However, although OC17 shares the conserved scaffold of the C-type lectins, it does not bind carbohydrates. Nevertheless, in vitro OC17 modifies the crystalline habit of calcium carbonate (CaCO 3 ) and the pattern of crystal growth at intervals of 5-200 g/ml. Determining the three-dimensional structure of OC17 contributes to a better understanding of the biological behavior of structurally related biomolecules and of the mechanisms involved in eggshell and other mineralization processes.

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“…Photo (2) showed the confluent area in eggshell of Hy-Line brown strain at early stage. Solomon (1999), found good shell ultrastructural beneficial high confluence reflects good attachment with membranes and caps. Data presented in Photo (3) indicated the late fusion in eggshell of Hy-Line W-36 strain at early production cycle.…”

Section: Scanning Electron Microscopy (Sem) Techniquementioning

confidence: 94%

Assessing Differences in the Quality Properties and Ultrastructure of Eggshell as Affected by Chicken Strain and Flock Age During Incubation Period

Rayan

Galal

Fathi

et al. 2023

Braz. J. Poult. Sci.

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Three hundred hatching eggs from two layer breeder flocks (Hy-Line Brown and hy-Line W-36; 150 each) were obtained at three different periods of laying cycle (early, middle and late stages). The hatching eggs were randomly taken to assess the impact of chicken strain, age, and hatching time on some eggshell measurements and structures. Results indicate that egg weight, shell percentage and shell thickness were significantly affected by strain, breeder age, and incubation time. Increasing rate of calcium absorption and utilization by the embryos during the embryonic stage clearly reflects the decrease occurred in eggshell strength. The differences of eggshell breaking strength during incubation time periods were 0.41 and 0.55 kg/cm 2 , the differences due to the incubation time may be due to the higher demand for calcium utilization in the late incubation period (18 days), observed on the10 th day of incubation. Brown eggs recorded significant higher calcium percentage in eggshell compared to the white ones. The brown breeder hens recorded significant higher total pores per egg compared to the white egg. Concerning eggshell ultrastructure, total thickness of the brown eggshell was significantly higher than those of the white eggshell by about 3.7%. All parameters, except for total thickness, were significantly affected by the interaction between strain and breeder age. Finally, we conclude that some traits including egg weight, shell percentage, shell thickness, calcium percentages in eggshell, and total pores per egg were significantly affected by strain, breeder age, and incubation time. Eggshell ultrastructure of brown egg was better compared to the white ones.

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An egg ist ein ei, es un huevo, est un oeuf

Cited by 15 publications

References 21 publications

Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix

Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix

Crystal Structure of Ovocleidin-17, a Major Protein of the Calcified Gallus gallus Eggshell

Assessing Differences in the Quality Properties and Ultrastructure of Eggshell as Affected by Chicken Strain and Flock Age During Incubation Period

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