An efficient two-photon fluorescent probe for human NAD(P)H:quinone oxidoreductase (hNQO1) detection and imaging in tumor cells

Abstract

A new quinone propionic acid locked TP fluorophore which can be used for human NAD(P)H:quinone oxidoreductase (hNQO1) detection was developed. The probe, TPQ, which displays high selectivity and anti-interference ability, was successfully applied to endogenous hNQO1 imaging and for the identification of different cancer cells.

Trimethyl Lock: A Multifunctional Molecular Tool for Drug Delivery, Cellular Imaging, and Stimuli‐Responsive Materials

“…Furthermore, in 2017, structurally similarp robes 109 and 111 (Scheme23) weres eparately reported by Jiang and coworkers [150] andY oon and co-workers, [151] respectively.B oth probesr eleasef luorophores that are suitable for two-photon fluorescencem icroscopy andt hat display long Stokess hifts as well as high signal-to-noise ratios.…”

Strategies for Tumor Hypoxia Imaging Based on Aggregation‐Induced Emission Fluorogens

“…Because of NTR, azoreductase, and DT‐diaphorase overexpression in the tumor hypoxic microenvironment, tumor hypoxia imaging fluorescent probes based on conventional fluorophores sensitive to these enzymes have been reported . The main principle to design the hypoxia‐responsive fluorescent probe is to attach a hypoxia‐responsive moiety as a marker to the fluorescent molecule, such as nitro and azo groups.…”

“…Because of NTR, azoreductase, and DT-diaphorase overexpression in the tumor hypoxicm icroenvironment, tumor hypoxia imaging fluorescent probesb ased on conventional fluorophores sensitive to these enzymes have been reported. [26][27][28][29] The main principle to designt he hypoxia-responsive fluorescent probe is to attach ah ypoxia-responsive moiety as a marker to the fluorescent molecule, such as nitro and azo groups.T his sectionf ocuses on typical samples of hypoxia-responsivef luorescent probes based on conventional fluorophores for hypoxia imaging, which can be instructive for the construction of hypoxia-responsive AIE probes.…”

An Activatable Photosensitizer Targeting Human NAD(P)H: Quinone Oxidoreductase 1

“…Rather, its high level has been mainly exploited for diagnostics and alternative therapeutic applications. For example, an array of fluorescent chemosensors have been developed to differentiate cancer cells from healthy cells, and in some instances identify metastases in animal models . Several groups have also developed hNQO1‐bioactivated anticancer agents (e.g., prodrugs) to enhance the cancer selectivity of DNA alkylators or other chemotherapeutic agents .…”

“…Rather,i ts high level has been mainlye xploited for diagnostics and alternative therapeutic applications.F or example, an array of fluorescent chemosensors have been developed to differentiate cancer cells from healthy cells, and in some instances identify metastases in animal models. [13][14][15][16][17][18][19][20][21][22][23][24][25] Severalg roupsh avea lso developed hNQO1-bioactivated anticancera gents(e.g.,p rodrugs) to enhancet he cancers electivity of DNA alkylators or other chemotherapeutic agents. [9,[26][27][28][29][30] Lastly,t heranostic prodrugs providingb oth real-time hNQO1 activity monitoring and anticancer drug release in as ingle molecule have also been reported.…”