An efficient system for catalyzing ester synthesis using a lipase from a newly isolatedBurkholderia cepaciastrain

Abstract

The synthesis of ethyl-oleate by the lipase from the newly isolated strain Burkholderia cepacia LTEB11 in three different systems has been studied Á immobilization on a hydrophobic support (Accurel EP 100 † ), encapsulation in reverse micelles, and direct addition of powdered free enzyme to the reaction medium. The immobilized enzyme performed best, giving a 70% ester yield in 10 h, this yield being fivefold greater than that obtained for reversed micelles, and two and a half times greater than that obtained for direct addition. An increase in the amount of immobilized enzyme preparation added gave a 100% ester yield in 3 h. The immobilized preparation was quite stable, giving a 100% yield of ethyl-oleate during 11 repeated reactions, and 50% yield after 24 reactions. These results suggest that the lipase of our strain of B. cepacia LTEB11 immobilized on Accurel has good potential for application in biocatalysis in organic media.

Miniemulsion as efficient system for enzymatic synthesis of acid alkyl esters

“…For this reason it is interesting to evaluate the performance of Amano lipase PS in comparison to other enzymes such as commercial C. rugosa lipase and F. s. pisi cutinase, produced as previously reported (Calado et al, 2003). These enzymes were also successfully used on the biosyntheses of alkyl esters mainly in the others different reactions media (de Barros et al, 2009b;Nagayama et al, 2002;Salum et al, 2008).…”

Burkholderia cepacia lipase: A versatile catalyst in synthesis reactions

“…BCL in its free form usually has low stability and its recovery is complex, making it virtually impossible to reuse. These features reduce the potential use of this lipase for practical purposes (Salum et al, ). Thus, the immobilization of lipase can not only improve its stability (and in some cases its activity), but also enable its recovery and reuse.…”

Characterization of Burkholderia lipase immobilized on celite carriers

“…C20 strain isolated from domestic food wastes. The Burkholderia lipase appeared to possess efficient lipolytic and alcoholytic activity (Salum et al, 2008), thereby having the potential for commercial applications. However, a free form of enzymes usually exhibits lower stability and is resistant to enzyme recovery and repeated operation.…”