AN EFFICIENT PROTOCOL TO PERFORM GENETIC TRACEABILITY OF TISSUE AND FOODS FROM Geoffroea decorticans

Abstract

the quality of a dnA isolation method depends, among others, on the target tissue and the metabolites therein. Geoffroea decorticans burkart (chañar) is a species that has nutritional and pharmacological potential. However, an effective method of DNA extraction capable of facilitating population studies and food genetic traceability has not been studied yet. the objective of the present work was to evaluate four methods of DNA extraction from leaves and chañar-based foods. the methods were evaluated based on yield, dnA purity, and molecular markers. the cci-p (ctAb/ chloroform-isoamylalcohol/pellet) method showed the highest yield of dnA obtained from leaves. However, the cpci-sc (ctAb/phenol-chloroform-isoamylalcohol/silica-column) method was the only one that resulted in acceptable dnA quality with both parameters (A260/A280 and A260/A230). the leaf dnA obtained with this method showed a greater amount of fragments with rApd, and an acceptable amount of fragments with issr. on the other hand, the cci-p method showed a higher yield of dnA from arrope de chañar (syrup). However, the cpci-sc method was the only one that had relatively better DNA quality, which allowed the amplification of molecular markers. Regarding chañar flour, the CPCI-SC method showed the highest yield, DNA quality and good amplification with molecular markers. Therefore, the CPCI-SC extraction method is efficient for obtaining DNA from different matrices, and can support studies for a possible designation of origin of chañar-based foods.

Genetic Characterization of an Endangered Chilean Endemic Species, Prosopis burkartii Muñoz, Reveals its Hybrids Parentage

“…DNA was extracted from fresh leaves, using a modified CTAB (cetyl trimethylammonium bromide) method described by Contreras et al [ 52 ]. Fine fresh leaf powder (100 mg) was obtained after grinding the tissue with liquid nitrogen.…”

Análisis taxonómico molecular de individuos del genero Lathyrus hallados en el extremo norte de Chile, mediante DNA barcoding

“…Sobre el origen de las especies endémicas Sudamericanas de Lathyrus, Asmussen & Liston (1998) sustentan la hipótesis de que éstas derivan de linajes norteamericanos, consistente con la teoría de Kupicha (1983), quien planteó la hipótesis de que Lathyrus se originó en latitudes altas del Viejo Mundo, en el Cretácico o Terciario temprano, de aquí las especies originales de Lathyrus con características de la sección Orobus migraron a Norteamérica a través de Groenlandia o de Asia a través de Alaska y luego a Sudamérica donde la sección Notolathyrus evolucionó. Esta ruta de dispersión de la sección Notolathyrus de Norte a Sudamérica sigue la teoría del elemento de flora holártica sobre el origen de especies andinas, así como la hipótesis boreotrópica biogeográfica (Cleef, 1979;Simpson & Todzia, 1990;Lavin & Luckow, 1993) En este estudio, la utilidad de las secuencias de nucleótidos de ITS, psbA-trnH y matK son efectivas como código de barras de ADN para la identificación de especies Lathyrus. La especie L. odoratus se encuentra naturalizada y creciendo de manera abundante en la Quebrada de Vitor, en una ubicación inhóspita, al oeste de Codpa y este del Valle de Chaca.…”

“…La extracción de ADN se realizó con el método CTAB/fenol-cloroformo-alcohol isoamílico/ columna de sílice, descrito por Contreras et al (2019). La amplificación del marcador nuclear ITS y marcadores plastídicos rpoC1, psbA y matK se realizaron en una reacción de PCR con las siguientes parejas de cebadores: rpoC1 (1F 5'-GTGGATACACTTCTTGATAATGG-3'; 4R 5'-CCATAAGCATATCTTGAGTTGG-3') ( Kress et al, 2005); ITS1-ITS2 (P674 5'-CCTTATCATTTAGAGGAAGGAG-3'; ITS-4 5'-TCCTCCGCTTATTGATATGC-3') (Stanford et al, 2000;Kress et al, 2005); matK (2.1f 5´-CCTATCCATCTGGAAATCTTAG-3´; 1326R 5'-TCTAGCACACGAAAGTCGAAGT-3') ( C u é n o u d e t a l .…”