An efficient protocol to generate placental chorionic plate-derived mesenchymal stem cells with superior proliferative and immunomodulatory properties

Abstract

Background Placenta-derived MSCs (P-MSCs) represent a promising tool for cell-based therapeutic applications. However, the increasing demand for P-MSCs in clinical trials makes high quality and large number of P-MSCs mandatory. Here, we aim to develop an efficient protocol for P-MSC isolation and culture. Methods The modified explant culture (MEC) method by combining an initial mild enzymatic reaction with the subsequent explant culture was developed to simultaneously produce various P-MSCs from the different regions of the placenta in serum-free medium (SFM). Its isolation efficiencies, cell yield, and proliferative capacity were compared with the conventional explant culture (EC) method. Furthermore, we determined whether functional properties of P-MSCs are affected by the used tissue-harvesting sites in terms of their proliferation, migration, and the immunomodulatory effect on macrophage. Results The MEC method achieved higher yield and shorter time in primary cell confluence in SFM compared with the conventional method. The harvested cells possessed the MSC characteristics and demonstrated significantly stronger proliferation ability. Importantly, MSCs derived from chorionic plate (CP-MSCs) were found to exhibit superior properties to the other P-MSCs in proliferation and migration capacity, maintaining the fetal origin over serial passages. Notably, CP-MSCs show stronger ability in regulating macrophage polarization from M1 to M2. Conclusion Our study developed an efficient and high-yield technique to produce high-quality P-MSCs from the placenta, hence serving as an optimal source of MSCs for clinical application.

Prophylactic therapy with human amniotic fluid stem cells improved survival in a rat model of lipopolysaccharide-induced neonatal sepsis through immunomodulation via aggregates with peritoneal macrophages

“…4 and Additional file 4 : Fig. S4) [ 43 , 44 ]. Moreover, hAFSCs spontaneously aggregated in the peritoneal cavity and formed spheroids (Fig.…”

Perinatal Derivatives: Where Do We Stand? A Roadmap of the Human Placenta and Consensus for Tissue and Cell Nomenclature

“…Several publications have dealt with various isolation methods of placental mesenchymal stromal cells and the comparison of these cells isolated from different placental sites, with the intention to find the most suitable ones. Isolation techniques vary from explant methods (Konig et al, 2015;Wu et al, 2018;Ma et al, 2019;Yi et al, 2020), enzymatic methods (Soncini et al, 2007;Kim et al, 2011;Yamahara et al, 2014;Kwon et al, 2016;Sardesai et al, 2017;Araújo et al, 2018;Ventura Ferreira et al, 2018;Chen et al, 2019;Yi et al, 2020), or the combination of both (Huang et al, 2019). Isolation methods using a modified explant culture technique combined with enzymatic treatment achieved higher cell yield and better proliferative capacity than conventional explant cultures (Huang et al, 2019).…”

Current Status and Future Prospects of Perinatal Stem Cells

“…The chorionic plate is made up of the amniochorionic membrane and the fetal vessels ( Figure 1 ). The stem cells are isolated from the closest region to the umbilical cord once the amniotic membrane is removed and the isolated cells have a mesenchymal type phenotype [ 53 ]. Chorionic plate MSC (CP-MSC) are positive for CD73, CD90, CD105, CD44, and CD166, and negative for CD45, CD34, CD14, CD19, and HLA-DR CP-MSCs express CD106 and CD54 [ 53 , 54 ].…”

“…The stem cells are isolated from the closest region to the umbilical cord once the amniotic membrane is removed and the isolated cells have a mesenchymal type phenotype [ 53 ]. Chorionic plate MSC (CP-MSC) are positive for CD73, CD90, CD105, CD44, and CD166, and negative for CD45, CD34, CD14, CD19, and HLA-DR CP-MSCs express CD106 and CD54 [ 53 , 54 ]. Chorionic plate MSC (CP-MSC) are of fetal origin and display superior proliferation, migration capacity, and immunomodulatory properties to MSC derived from other perinatal tissues such as umbilical cord (UC-MSC), chorionic villi (CV-MSC), and decidua (DMSC) [ 53 , 55 ].…”

“…Chorionic plate MSC (CP-MSC) are positive for CD73, CD90, CD105, CD44, and CD166, and negative for CD45, CD34, CD14, CD19, and HLA-DR CP-MSCs express CD106 and CD54 [ 53 , 54 ]. Chorionic plate MSC (CP-MSC) are of fetal origin and display superior proliferation, migration capacity, and immunomodulatory properties to MSC derived from other perinatal tissues such as umbilical cord (UC-MSC), chorionic villi (CV-MSC), and decidua (DMSC) [ 53 , 55 ]. In vitro differentiation assays show that CP-MSC are able to differentiate into adipogenic, osteogenic, chondrogenic, and hepatogenic lineages.…”