An efficient protocol for micropropagation of old cypress of Abarkuh (Cupressus sempervirens var. horizontalis [Mill.]) under in vitro condition

Khamushi, Mahdieh; Dehestani-Ardakani, Maryam; Zarei, Abdolkarim; Aliabad, Kazem Kamali

doi:10.1007/s11240-019-01645-z

Cited by 16 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For shoot elongation, a low concentration of activated charcoal (0.1 g l −1 ) in regulator-free medium had a positive effect (Capuana and Giannini 1997). Subsequent rooting is the most problematic phase of the multiplication procedure (Khamushi et al 2019). Although the explants from young plants root easily, rooting efficiency of adult material is very low.…”

Section: Micropropagation In Vitromentioning

confidence: 99%

See 1 more Smart Citation

The endangered Saharan cypress (Cupressus dupreziana): do not let it get into Charon’s boat

Lábusová

Konrádová

Lipavská

2020

Planta

View full text Add to dashboard Cite

show abstract

Section: Micropropagation In Vitromentioning

confidence: 99%

“…Dalichaouch (1997) also achieved satisfactory growth of shoots with 0.1-0.5 mg l −1 BAP but without any rooting. Worth mentioning in this context is the fact that rooting induction also remained as a problematic step in relatively well-managed micropropagation of C. sempervirens (Khamushi et al 2019).…”

Section: State-of-art Of C Dupreziana Micropropagationmentioning

confidence: 99%

The endangered Saharan cypress (Cupressus dupreziana): do not let it get into Charon’s boat

Lábusová

Konrádová

Lipavská

2020

Planta

View full text Add to dashboard Cite

show abstract

“…Micropropagation techniques can be used for conservation and producing healthy and disease-free plants (Wang et al 2016;Khamushi et al 2019). In addition, compared to conventional cloning methods, micropropagation has a much higher capacity to multiply the target plant material all-year round (Dobránszki, Teixeira da Silva 2010;Fan et al 2017).…”

mentioning

confidence: 99%

“…In recent years, apple rootstock propagation has achieved great progress, and the rapid propagation by tissue culture has been successful in some rootstocks (Sicurani et al 2001;Kermani et al 2009;Dai et al 2014). Studies suggested that different genotypes of apple rootstock have different micropropagation systems, such as the explant choice, the establishment of in vitro culture, sterilisation, and rooting media (Sarwar et al 1997;Aroonpong et al 2015;Khamushi et al 2019). These different responses of the genotypes might be associated with varied levels of endogenous growth regulators (Teixeira da Silva et al 2019).…”

mentioning

confidence: 99%

Establishment of an efficient micropropagation system in enhancing rooting efficiency via stem cuttings of apple rootstock M9T337

Shi¹,

Dong²,

Song³

et al. 2021

Hort. Sci. (Prague)

View full text Add to dashboard Cite

Rootstocks play a vital role in regulating the environmental adaptability and controlling the growth and development of apple trees. M9T337, an excellent apple rootstock widely used in commercial orchards, could confer dwarf tree architectures, early fruiting and suitability for high-density planting. However, the rooting ability of M9T3337 is low when it is vegetatively propagated, and researchers have not yet established an efficient micropropagation system. The present study systematically evaluated the multiplication in adventitious shoots and the in vitro formation of adventitious roots to determine the effects of the culture media and plant growth regulators of M9T337 and a rapid micropropagation system was developed. For the shoot multiplication, the highest multiplication index of 3.93 was obtained on Murashige and Skoog (MS) media supplemented with 2.0 mg/L 6-BA, 0.1 mg/L NAA and 0.3 mg/L GA3 from 12 combinations of 6-BA and NAA. Stronger and taller adventitious shoots were grown on MS supplemented with 1.8 mg/L 6-BA and 0.5 mg/L NAA. The optimal media with 100% rooting was obtained using ½ MS supplemented with 0.3 mg/L IBA or MS supplemented with 0.6 mg/L IBA for the rooting induction, resulting in mean rooting numbers of 13.00 and 11.33, respectively. Additionally, the effect on rooting of adding 0.3 mg/L IBA or not on the 1/2 MS and MS media was compared; the results suggested that an appropriate IBA concentration was the key to successful rooting. The rooted plantlets were acclimatised in a shaded greenhouse with an 84% survival rate. The established micropropagation system could be used for the rapid propagation of M9T337 for commercial production.

show abstract

“…virens var. horizontalis )의 기내증식을 위해 BA와 IBA를 처리 하여 다신초지로 부터 줄기증식을 연구한 연구가 최근 보고 되었다 (Khamushi et al 2019). 본 연구에서는 편백의 다신초 지 유도를 위한 적정 사이토키닌류의 종류 및 농도 확인, 또 한 식물체의 기내 생육을 저해하는 갈변화 현상을 해결하기 위한 몇 가지 항갈변화제 처리효과 연구를 함께 수행하였다.…”

unclassified

In vitro plantlets regeneration by multi-shoots induction and rooting in Chamaecyparis obtusa

2019

View full text Add to dashboard Cite

A protocol for the in vitro propagation of Chamaecyparis obtusa was established in the present study. Multi-shoots were initiated from apical shoot explants from germinants after 10 weeks of culture on Litvay medium (LM) supplemented with different concentrations of cytokinin. The effects of pre-treatment with high concentrations of cytokinin and varying concentrations (0.2 to 5.0 mg/L) of zeatin on in vitro shoot elongation and shoot multiplication were investigated. Optimal shoot growth was achieved on LM medium, with over 10-mm shoots after 10 weeks of culture. In the anti-browning tests, ethanesulfonic acid triggered the least browning in the shoot tips. The highest multi-shoot induction was observed in the 0.5-mg/L zeatin treatments, which yielded 80% induction of shoots after 10 weeks of culture, and maximum shoot elongation was observed in the LM basal medium without the hormone. The highest rooting rates were 65% under 0.2 mg/L indole-3-butyric acid.

show abstract

An efficient protocol for micropropagation of old cypress of Abarkuh (Cupressus sempervirens var. horizontalis [Mill.]) under in vitro condition

Cited by 16 publications

References 11 publications

The endangered Saharan cypress (Cupressus dupreziana): do not let it get into Charon’s boat

The endangered Saharan cypress (Cupressus dupreziana): do not let it get into Charon’s boat

Establishment of an efficient micropropagation system in enhancing rooting efficiency via stem cuttings of apple rootstock M9T337

In vitro plantlets regeneration by multi-shoots induction and rooting in Chamaecyparis obtusa

Contact Info

Product

Resources

About