An efficient protocol for in vitro regeneration of peanut (Arachis hypogaea L.) cultivar L14

Abstract

The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.

Enhancement of resistance against fungal pathogens in peanut (Arachis hypogaea L.) cultivar L14 by heterologous expression of gene encoding chitinase 42 kDa from Trichoderma asperellum SH16

“…Peanut (A. hypogaea L.) cultivar L14 from the Vietnam Academy of Agricultural Sciences' Field Crops Research Institute was utilized in this study (Hoa et al 2021). The seed coat was removed after being soaked in 70% EtOH for 1 min and sterilized with 65% NaClO/100 µL Tween 20 solution for 10 min.…”

Red fluorescence protein (DsRed2) promotes the screening efficiency in peanut genetic transformation

“…For the particle bombardment method, genes are transferred to embryogenic callus in peanut, and transformed plants are regenerated from somatic embryogenesis (Krishna et al, 2015). For A. tumefaciens-mediated transformation, leaflet (Dolce et al, 2018), de-embryonated cotyledon (Hoa et al, 2021;Marka and Nanna, 2021), cotyledonary node (Lamboro et al, 2022) and hypocotyl (Yan et al, 2015) are the main explants, and transgenic plants are regenerated from somatic embryogenesis or organogenesis (Kundu and Gantait, 2018). However, the efficiency of transformation in peanut is still as low as 0.2%-3.3% (Rohini and Rao, 2000), which is determined by the regeneration ability of the explant, host genotype, vectors, screening efficiency and some other factors (Krishna et al, 2015).…”