An efficient protocol for clonal regeneration and excised root culture with enhanced alkaloid content in Thalictrum foliolosum DC.— an endemic and important medicinal plant of temperate Himalayan region

Mishra, Manoj Kumar; Pandey, Shatrujeet; Misra, Pratibha; Niranjan, Abhishek; Srivastava, Alaka

doi:10.1016/j.indcrop.2020.112504

Cited by 14 publications

(6 citation statements)

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“…Improving the yield of active ingredients by optimizing the processing methods is a good way to achieve the above goals ( Mishra et al, 2020 ; Nabet et al, 2019 ; Wang et al, 2018a ). Typically, the active ingredients in medicinal plants are secondary metabolites that are closely related to cultivation methods, field management, harvest time, primary processing in the producing area, deep processing, storage, and transportation, etc.…”

Section: Introductionmentioning

confidence: 99%

Growth years and post-harvest processing methods have critical roles on the contents of medicinal active ingredients of Scutellaria baicalensis

Bai

Yang

Cao

et al. 2020

Industrial Crops and Products

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Section: Introductionmentioning

confidence: 99%

Growth years and post-harvest processing methods have critical roles on the contents of medicinal active ingredients of Scutellaria baicalensis

Bai

Yang

Cao

et al. 2020

Industrial Crops and Products

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“…Andrographis paniculata, Withania somnifera, Panax ginseng, Panax quinquefolium, Coptis japonica and Carica papaya have been widely used in natural medicine to treatment of various chronic disorders [5,21]. Similarly, a lesser explored medicinal herb T. foliolosum widely used as traditional medicine in gastric problem, bloating, toothache, abdominal colic pain and piles (hemorrhoids) [7,22]. Plants contain a variety of bioactive compounds, such as alkaloids, flavonoids, terpenoids, sterols and The data are presented as the mean ± the standard deviation.…”

Section: Discussionmentioning

confidence: 99%

“…Plant samples were extracted and subjected to further analysis using High-Performance Liquid Chromatography (HPLC) with a gradient method for identifying bioactive compounds, following the protocol established by Mishra et al [6,7]. The analysis was performed using a Shimadzu (Japan) HPLC Prominence system comprising a 20 μL sample loop, a PDA SPD M 20 A photodiode array system, an LC-20AD dual pump system, and a SIL-20 AC Autoinjector with a cooler.…”

Section: Hplc Methodsmentioning

confidence: 99%

“…Later all extracted materials were pooled in round bottom flask and evaporated at 50 °C using a rotary evaporator (Buchi, USA) under low pressure. Finally, the dried extracted plant materials were dissolved in methanol and filtered through a 0.45 µm Mishra Future Journal of Pharmaceutical Sciences (2024) 10:70 filter and stored at 4 °C for subsequent HPLC analysis [6,7]. The acidic-methanol extract of T. foliolosum had a yield of 27.42%, 21.10% and 16.24% of the extracted root, leaves and stem respectively.…”

Section: Extraction Of Plant Materials For Hplc Analysismentioning

confidence: 99%

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Phytochemical analysis and evaluation of antibacterial activity of various extracts from leaves, stems and roots of Thalictrum foliolosum

Mishra

2024

Futur J Pharm Sci

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Background Thalictrum foliolosum, a member of the Ranunculus family, is recognized for its therapeutic potential in addressing gastric issues, dyspepsia, tooth pain, abdominal colic pain, and piles. The diverse array of secondary metabolites present in the plant contributes to these therapeutic applications. This study aims to uncover and quantify the bioactive secondary metabolites found in the unexplored leaves, stems, and roots of T. foliolosum. Additionally, we also aimed to evaluate the antibacterial activity and MIC values of these extracts against a panel of pathogenic bacteria, such as pathogenic strains, including Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutant and Staphylococcus aureus. Result HPLC analysis suggested all examined compounds were found significantly more in root parts of plant. To determine the potential antimicrobial activity of different plant parts result suggested chloroform fraction of root most effective with variable potency against each examined pathogen at 25–100 µg/ml extracts which indicated rich content of berberine in this fraction. Minimum MIC (121.26 µg/mL) of the chloroform fraction of the root was also supported the results. Fatty acid methyl ester analysis by gas chromatography revealed that the stem contained high levels of fatty acids, such as palmitic acid, stearic acid, and linolenic acid, all of which have antibacterial properties. Conclusion The potential antimicrobial activity of extracts of various plant parts strongly supports the T. foliolosum plant's widespread use in folk medicine for the treatment of various chronic diseases and adulterants with various associated medicinal plant species.

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“…One potential strategy suggested in studies on biodiversity conservation of threatened species consists of the preservation of tissue culture, an in vitro cultivation of isolated live tissue, enabling the propagation of species facing difficulties with natural reproduction ( Paiva and Paiva, 2001 ; Santos et al, 2019 ). Protocols to apply tissue culture techniques for conservation of endangered species have been successfully developed (e.g., Sherif et al, 2018 ; Choudary et al, 2020 ; Lerin et al, 2021 ; Mishra et al, 2020 ), and such an approach has been used to conserve the narrow endemic species Styphelia longissima ( Thomas et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

One Step Away From Extinction: A Population Genomic Analysis of A Narrow Endemic, Tropical Plant Species

Teixeira

Nazareno

2021

Front. Plant Sci.

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Intraspecific genetic variation plays a fundamental role in maintaining the evolutionary potential of wild populations. Hence, the assessment of genetic diversity patterns becomes essential to guide biodiversity conservation policies, particularly for threatened species. To inform management strategies for conservation of Mimosa catharinensis – a narrow endemic, critically endangered plant species – we identified 1,497 unlinked SNP markers derived from a reduced representation sequencing method (i.e., double digest restriction site associated DNA sequencing, or ddRADseq). This set of molecular markers was employed to assess intrapopulation genetic parameters and the demographic history of one extremely small population of M. catharinensis (N=33) located in the Brazilian Atlantic Forest. Contrary to what is expected for narrow endemic and threatened species with small population sizes, we observed a moderate level of genetic diversity for M. catharinensis [uHE(0%missing data)=0.205, 95% CI (0.160, 0.250); uHE(30%missing data)=0.233, 95% CI (0.174, 0.292)]. Interestingly, M. catharinensis, which is a lianescent shrub with no indication of seed production for at least two decades, presented high levels of outcrossing [t(0%missing data)=0.883, SE±0.0483; t(30%missing data)=0.909, SE±0.011] and an apparent absence of inbreeding [F(0%missing data)=−0.145, 95% CI (−0.189, −0.101); F(30%missing data)=−0.105, 95% CI (−0.199, −0.011)]. However, the reconstruction of demographic history of M. catharinensis indicated that the population should be suffered a recent bottleneck. Our population genomic study tackles a central issue in evolution and conservation biology and we expect that it will be useful to help safeguard the remaining genetic diversity reported for this unique genetic resource.

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An efficient protocol for clonal regeneration and excised root culture with enhanced alkaloid content in Thalictrum foliolosum DC.— an endemic and important medicinal plant of temperate Himalayan region

Cited by 14 publications

References 30 publications

Growth years and post-harvest processing methods have critical roles on the contents of medicinal active ingredients of Scutellaria baicalensis

Growth years and post-harvest processing methods have critical roles on the contents of medicinal active ingredients of Scutellaria baicalensis

Phytochemical analysis and evaluation of antibacterial activity of various extracts from leaves, stems and roots of Thalictrum foliolosum

One Step Away From Extinction: A Population Genomic Analysis of A Narrow Endemic, Tropical Plant Species

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