An efficient molecular technique for sexing tiger pufferfish (fugu) and the occurrence of sex reversal in a hatchery population

Matsunaga, Takayoshi; Ieda, Risa; Kuroyanagi, Miwa; Suzuki, Shigenori; Suetake, Hiroaki; Tasumi, Satoshi; Suzuki, Yuzuru; Miyadai, Toshiaki; Kikuchi, Kiyoshi

doi:10.1007/s12562-014-0768-0

Cited by 21 publications

(16 citation statements)

References 44 publications

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“…Maturation in males was concomitant with maturation in females. These findings were similar to those reported by Hamasaki et al (2013), Matsunaga et al (2014). In the ovary, the level of tetrodotoxin was reported to be highest (Thattiyaphong et al, 2014;Itoi et al, 2017;Acar et al, 2017).…”

Section: Discussionsupporting

confidence: 90%

“…Oogonia of T. flavimaculosus divided and turned into prenucleolus stage, which subsequently grow within follicles, formed cortical alveoli, entered vitellogenesis underwent maturation and finally ovulated. The changes that occurred during these phases were similar to those reported for other pufferfish (Hamasaki et al, 2013;Matsunaga et al, 2014). Based on histological observations, maturation in T. flavimaculosus occurred from June to October, which was suggested as a spawning season.…”

Section: Discussionsupporting

confidence: 81%

“…Similar to the present study, in both studies sex were separated and population consisted of dioecious specimens. In a study by Matsunaga et al (2014), the sex ratio of tiger pufferfish was highly skewed towards males. While 77 fish had ovaries, 201 fish contained testes.…”

Section: Discussionmentioning

confidence: 97%

“…While 77 fish had ovaries, 201 fish contained testes. Moreover, 15 fish possessed both testicular and ovarian tissue (Matsunaga et al, 2014). Currently, there is no available data on the sex ratio of T. flavimaculosus.…”

Section: Discussionmentioning

confidence: 99%

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Maturation and Gonad Development of Yellowspotted Puffer Torquigener flavimaculosus (Osteichthyes: Tetraodontidae) from Iskenderun Bay, North-eastern Mediterranean

Yalnız¹,

Turan²,

Doğdu³

2017

Natural and Engineering Sciences

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Marine pufferfish of the family Tetraodontidae accumulate the highest levels of tetrodotoxin (TTX) in the ovary. The level of TTX accumulation is reported to differ between males and females and fluctuate through gonadal development and maturation stages. Therefore, in the present work, maturation and gonad development of Yellowspotted Puffer (Torquigener flavimaculosus) from Iskenderun Bay, North-eastern Mediterranean were investigated histologically and morphologically. Mean length and weight of specimens were 12.1±0.58 cm and 20.25±0.17 g respectively. Histological examination of the gonads showed that maturation occurs in every single male T. flavimaculosus collected in summer 2017. Moreover, vitellogenic and matured oocytes were also consistently found in every female collected during the summer months. These data suggest that both sexes are reproductively active at the same time of the year. Where the spawning season for both males and females was detected in summer. T. flavimaculosus was found to be dioecious. Five and six developmental stages were indicated for testis and ovaries, respectively. The developmental pattern of ovaries was categorized as the asynchronous or group synchronous type.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Maturation and Gonad Development of Yellowspotted Puffer Torquigener flavimaculosus (Osteichthyes: Tetraodontidae) from Iskenderun Bay, North-eastern Mediterranean

Yalnız¹,

Turan²,

Doğdu³

2017

Natural and Engineering Sciences

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“…Using extensive genetic mapping and association mapping, a missense single nucleotide polymorphism (SNP) in Amhr2 gene was identified to be responsible for genetic sex determination (Kamiya et al., ). Based on this SNP, a high‐resolution melting analysis for effectively identifying the gender of puffer fish has recently been developed (Matsunaga et al., ).…”

Section: Introductionmentioning

confidence: 99%

Effects of estradiol-17β on survival, growth performance, gonadal structure and sex ratio of the tiger puffer,Takifugu rubripes (Temminck & Schlegel, 1850), fingerlings

et al. 2018

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Systematic research was performed to assess the survival, growth performance, gonadal structure and sex ratio during and after sex reversal induced by estradiol‐17β in tiger puffers. The fish of 20 days after hatching (dah) were immersed in different doses (0.2, 2, 20, 100 ppb) for 60 days. There were significant differences in survival rates among groups (p < .05). With the increase in the estradiol‐17β dose, the survival rates of tiger puffers decreased, revealing a linear regression relationship with log10 (estradiol + 1; p < .05). The growth tendency of fish in the low‐dose group was similar to those in the control group over the same period. The growth in the 20 ppb dose group was significantly slower than in the low‐dose and control groups in fish of same age (p < .05), but was significantly faster than in the 100 ppb dose group (p < .05) after 60 days. Intersex fish were categorized into intersex I, II and III types, according to the feminization degree from low to high. Phenotypic intersex puffer fish were found in the 2, 20 and 100 ppb dose groups. Intersex ratios in 2, 20 and 100 ppb were 10%, 43.3% and 46.7%, respectively, the ratios except the ones in 2 ppb group being significantly higher than those in the control group (p < .05). Moreover, 100.0% male induction for intersex II and III types occurred in 20 and 100 ppb groups. Together, these results indicated that estradiol‐17β at a dose of 20 ppb was most effective for pseudo‐female production.

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Population Consequences of Releasing Sex‐Reversed Fish

Wedekind

2018

Sex Control in Aquaculture

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An efficient molecular technique for sexing tiger pufferfish (fugu) and the occurrence of sex reversal in a hatchery population

Cited by 21 publications

References 44 publications

Maturation and Gonad Development of Yellowspotted Puffer Torquigener flavimaculosus (Osteichthyes: Tetraodontidae) from Iskenderun Bay, North-eastern Mediterranean

Maturation and Gonad Development of Yellowspotted Puffer Torquigener flavimaculosus (Osteichthyes: Tetraodontidae) from Iskenderun Bay, North-eastern Mediterranean

Effects of estradiol-17β on survival, growth performance, gonadal structure and sex ratio of the tiger puffer,Takifugu rubripes (Temminck & Schlegel, 1850), fingerlings

Population Consequences of Releasing Sex‐Reversed Fish

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