An Efficient Micropropagation System for the Vulnerable Wild Apple Species, Malus sieversii, and Confirmation of Its Genetic Homogeneity

Kakimzhanova, Almagul; Dyussembekova, Damira; Nurtaza, Aidana; Yessimseitova, Assel; Shevtsov, Alexandr; Lutsay, Viktoriya; Ramankulov, Yerlan; Kabieva, Saltanat

doi:10.1007/s10341-022-00720-8

Cited by 3 publications

(1 citation statement)

References 58 publications

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“…Simple sequence repeats markers were employed to assess the genetic uniformity of the micropropagated plants of Helianthus verticillatus Small, and their genetic stability was confirmed between the regenerants and their respective donor plants [ 95 ]. Kakimzhanova et al [ 96 ] reported that simple sequence repeat analysis confirmed the reliability (genetic homogeneity) of their protocol for efficient large-scale micropropagation of Malus sieversii . In another species of Malus , Malus niedzwetzkyana , Nurtaza et al [ 97 ] using simple sequence repeat primers, detected no somaclonal variation between the mother plant and the micropropagated clones of this endangered species.…”

Section: Discussionmentioning

confidence: 97%

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Ioannidis¹,

Tomprou²,

Mitsis³

et al. 2022

Plants

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Simple sequence repeat (SSR) markers were used to evaluate the genetic stability of the acclimatized micropropagated and regenerated plants of a high cannabidiol (H-CBD) and a high cannabigerol (H-CBG) variety of Cannabis sativa L. Shoot regeneration and proliferation were achieved by culturing calli in Murashige and Skoog basal medium (MS) supplemented with several concentrations of 6-benzyladenine (BA) or thidiazuron (TDZ). Calli derived mostly from stem explants, rather than leaves, cultured on MS supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) or combination of kinetin (KIN) with 1-Naphthaleneacetic acid (NAA) or 2,4-D. Rooting of the regenerated plantlets accomplished on half-strength MS medium supplemented with indole-3-butyric acid (IBA). Previous studies performed have developed an efficient in vitro micropropagation protocol for mass production. Both in vitro methodologies can be employed in genetic breeding via molecular techniques. The genetic stability of micropropagated and regenerated plants was accomplished using twelve SSR primer pairs that produced reproducible and clear bands, ranging from 90 to 330 bp in size, and resulted in amplification of one or two alleles, corresponding to homozygous or heterozygous individuals. The SSR amplification products were monomorphic across all the micropropagated and regenerated plants and comparable to mother plants. The monomorphic banding pattern confirmed the genetic homogeneity of the in vitro cultured acclimatized and mother plants as no somaclonal variation was detected in clones for these specific SSRs. Our results evidently suggest that the developed culture protocols for in vitro multiplication is appropriate and applicable for clonal mass propagation of the C. sativa varieties and demonstrate the reliability of this in vitro propagation system.

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Section: Discussionmentioning

confidence: 97%

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Ioannidis¹,

Tomprou²,

Mitsis³

et al. 2022

Plants

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Comparative genetic assessment of somatic embryo– and seed-derived plants of two arabica hybrid coffee cultivars using SRAP and SCoT molecular markers and organellar and nuclear genes sequencing

Mishra,

Huded,

Jingade

et al. 2024

In Vitro Cell.Dev.Biol.-Plant

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Clonal micropropagation of apple trees

Tashmatova,

Matsneva,

Khromova

2023

Vestnik of the Russian agricultural science

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The purpose of the review is to analyze the state of knowledge of the issue of apple tree reproduction in Russia and abroad. Of all the methods of tissue culture, clonal micropropagation is the most studied, which has a number of advantages in comparison with traditional vegetative multiplication: obtaining healthy material, rapid reproduction, obtaining genetically homogeneous material, the possibility of releasing material throughout the year. Micropropagation has played an important role in the production of healthy, disease-free plants and in the rapid multiplication of apple varieties and rootstocks. This review shows that over the past decades, foreign and domestic researchers, including scientists of our institute, have developed a large number of methods for cultivating of varieties and rootstocks of apple trees. Due to the heterozygosity of the genus Malus, protocols for specific gene types are often developed. The conducted research analysis showed that the successful reproduction of apple trees is influenced by external and internal factors (genotype, physiological state, components of nutrient media, conditions of adaptation of micro-plants). The review reflects various approaches to solving such problems as obtaining an aseptic culture at the initiation stage, reducing the negative effect of phenol oxidation products, increasing the proliferative activity of explants using various nutrient media, as well as natural and synthetic growth hormones - cytokinins, problems of stimulating root formation as a result of the use of various types of auxins and methods of exposure, the development of conditions for the adaptation of microplants of varieties and rootstocks of apple trees in vivo. The emergence of new varieties will require the development of new cultivation conditions.

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An Efficient Micropropagation System for the Vulnerable Wild Apple Species, Malus sieversii, and Confirmation of Its Genetic Homogeneity

Cited by 3 publications

References 58 publications

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Genetic Evaluation of In Vitro Micropropagated and Regenerated Plants of Cannabis sativa L. Using SSR Molecular Markers

Comparative genetic assessment of somatic embryo– and seed-derived plants of two arabica hybrid coffee cultivars using SRAP and SCoT molecular markers and organellar and nuclear genes sequencing

Clonal micropropagation of apple trees

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