An efficient micropropagation protocol for <i>Vernonia amygdalina</i> Delile – An economically valuable shrub

Joshi, Bhavana; Bhandari, Aakriti; Panwar, G. S.

doi:10.1080/10496475.2020.1728601

Cited by 5 publications

(1 citation statement)

References 13 publications

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“…Similarly, Kaviani et al (2019) cultivated the apical meristems of Aglaonema widuri in MS media with diverse growth regulating agent and obtained the most number of nodes in cultures with 4.0 mg/l BAP + 0.5 mg/l TDZ + 0.1 mg/l NAA. Joshi et al (2020) cultivated the shoot tip explants of Vernonia amygdalina Delile in the nutrient media supported with BAP, TDZ and Kn individually and associated with NAA, and obtained a large number of shoots (25.42 ± 2.5 shoots/explant) in nutritional medium strengthened with 4.4 µM BAP + 1.59 µM NAA. Raghavendra et al (2019) kept Cicer arietinum L. in an environment fortified with diverse doses of BAP, Kn, NAA and IAA for in vitro shoot regeneration and achieved the best shoot differentiation in a nutritional medium including 4.0 mg/l BAP + 2.0 mg/l NAA.…”

Section: Resultsmentioning

confidence: 99%

High Efficiency Plant Regeneration From Shoot Tip Explants Of Staurogyne Repens (Nees) Kuntze

Doğan

2022

Bangladesh J. Bot.

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A method for tissue culture of micropropagation of aquatic plant Staurogyne repens (Nees) Kuntze has been developed. Different phytohormones were investigated to optimize the growth of explants. The shoot tip explants of S. repens were placed in MS nutrient medium including BAP (0.30-1.50 mg/l) + TDZ (0.10 mg/l) + NAA (0.10 mg/l) and Kn (0.30-1.50 mg/l) + TDZ (0.10 mg/l) + NAA (0.10 mg/l). Shoot regeneration frequencies were changed as 77.77-100% and 66.66-100% in cultivation medium augmented with BAPTDZ- NAA and Kn-TDZ-NAA, respectively. In the growing media with BAP, the maximum shoot number (14.44 shoot/explant) was obtained in MS medium with 1.20 mg/l BAP + 0.10 mg/l TDZ + 0.10 mg/l NAA. In Kn applications, the highest shoot number (9.50 shoot/explant) was recorded in growing medium with 0.30 mg/l Kn + 0.10 mg/l TDZ + 0.10 mg/l NAA. The longest shoots (2.33 cm) was reached in nutrient medium with 0.90 mg/l Kn + 0.10 mg/l TDZ + 0.10 mg/l NAA. Regenerated plants rooting at 0.25 mg/l IAA have been effectively acclimated to aquarium conditions. Bangladesh J. Bot. 51(4): 697-704, 2022 (December)

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Section: Resultsmentioning

confidence: 99%

High Efficiency Plant Regeneration From Shoot Tip Explants Of Staurogyne Repens (Nees) Kuntze

Doğan

2022

Bangladesh J. Bot.

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Micropropagation of Tricholepis roylei Hook. F.—a point endemic species of the Western Himalaya

Panwar

Joshi

2020

Journal of Genetic Engineering and Biotechnology

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Background Tricholepis roylei Hook. f. is a bushy annual shrub of the Asteraceae family and point endemic species of the Western Himalaya. T. roylei is a critically endangered species and factors like poor seed germination and habitat destruction are further rendering the species towards extinction. Therefore, the present investigation was designed to document the seed germination potential of the species and to establish a reproducible in vitro propagation and mass multiplication protocol for the ex situ conservation of T. roylei germplasm. Results Seeds of T. roylei were collected from Sangdha, Himachal Pradesh, India, and were sown in different substrates under open and controlled conditions. Though the overall seed germination potential of the species was reported to be very low and maximum 45% germination was observed in cocopeat substrate after 26 days of incubation. Half-strength Murashige and Skoog (MS) medium enriched with 6-benzylaminopurine (4.4 μM l −1 ) and naphthalene acetic acid (1.0 μM l -1 ) was observed to be the optimum medium for shoot induction in shoot tip explants of T. roylei . Maximum 98.89% shoot formation was observed with 28.42 shoots per culture and 4.4-cm shoot length, respectively. The healthy shoots (4.0 cm) were transferred onto rooting media (1/4, 1/2 and full MS) and roots were developed after 8 weeks of incubation in the half-strength MS medium. Half MS augmented with 4.9 μM l −1 indole butyric acid was observed to be optimum for the root development and an average of 10.2 roots per shoot with 4.0-cm length was obtained. Rooted plantlets were successfully acclimatized under greenhouse conditions and subsequently established in the field, with a recorded survival rate of 90%. The plants acclimatized to the open environment were also planted in the wild under the habitat rehabilitation and species recovery programme. Conclusion The seed germination study envisages that the seed germination potential of the species is very poor and might be one of the probable factors responsible for the shrinkage of T. roylei population in the wild. The standardized micropropagation protocol can be helpful for the ex situ conservation of germplasm and rehabilitation of species in the wild. Moreover, the study could be helpful in elucidating the phytochemical and molecular analysis of species.

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Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze

2021

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An efficient micropropagation protocol for Vernonia amygdalina Delile – An economically valuable shrub

Cited by 5 publications

References 13 publications

High Efficiency Plant Regeneration From Shoot Tip Explants Of Staurogyne Repens (Nees) Kuntze

High Efficiency Plant Regeneration From Shoot Tip Explants Of Staurogyne Repens (Nees) Kuntze

Micropropagation of Tricholepis roylei Hook. F.—a point endemic species of the Western Himalaya

Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze

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