An Efficient Method of Pennisetum × advena ‘Rubrum’ Plantlets Production Using the Temporary Immersion Bioreactor Systems and Agar Cultures

Pożoga, Mariusz; Olewnicki, Dawid; Wójcik‐Gront, Elżbieta; Latocha, Piotr

doi:10.3390/plants12071534

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…vetiver grass [8], Cymbopogon schoenanthus [9], switchgrass (Panicum virgatum L.) [10], finger millet (Eleusine coracana (L.) Gaertn.) [11], Pennisetum purpureum [12], Pennisetum × advena 'Rubrum' [13], and cereals; as well as rice [14], wheat [15], wheat, durum wheat, oat, barley, triticale [16], and sorghum [17].…”

Section: Introductionmentioning

confidence: 99%

The Cytokinins BAP and 2-iP Modulate Different Molecular Mechanisms on Shoot Proliferation and Root Development in Lemongrass (Cymbopogon citratus)

Cárdenas-Aquino,

Camas-Reyes,

Valencia-Lozano

et al. 2023

Plants

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The known activities of cytokinins (CKs) are promoting shoot multiplication, root growth inhibition, and delaying senescence. 6-Benzylaminopurine (BAP) has been the most effective CK to induce shoot proliferation in cereal and grasses. Previously, we reported that in lemongrass (Cymbopogon citratus) micropropagation, BAP 10 µM induces high shoot proliferation, while the natural CK 6-(γ,γ-Dimethylallylamino)purine (2-iP) 10 µM shows less pronounced effects and developed rooting. To understand the molecular mechanisms involved, we perform a protein–protein interaction (PPI) network based on the genes of Brachypodium distachyon involved in shoot proliferation/repression, cell cycle, stem cell maintenance, auxin response factors, and CK signaling to analyze the molecular mechanisms in BAP versus 2-iP plants. A different pattern of gene expression was observed between BAP- versus 2-iP-treated plants. In shoots derived from BAP, we found upregulated genes that have already been demonstrated to be involved in de novo shoot proliferation development in several plant species; CK receptors (AHK3, ARR1), stem cell maintenance (STM, REV and CLV3), cell cycle regulation (CDKA-CYCD3 complex), as well as the auxin response factor (ARF5) and CK metabolism (CKX1). In contrast, in the 2-iP culture medium, there was an upregulation of genes involved in shoot repression (BRC1, MAX3), ARR4, a type A-response regulator (RR), and auxin metabolism (SHY2).

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Section: Introductionmentioning

confidence: 99%

The Cytokinins BAP and 2-iP Modulate Different Molecular Mechanisms on Shoot Proliferation and Root Development in Lemongrass (Cymbopogon citratus)

Cárdenas-Aquino,

Camas-Reyes,

Valencia-Lozano

et al. 2023

Plants

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A Temporary Immersion System as a Tool for Lowering Planting Material Production Costs Using the Example of Pennisetum × advena ‘Rubrum’

Pożoga,

Olewnicki,

Latocha

2024

Agriculture

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The aim of the study was to compare the variable costs of planting material production using the example of vitro cultures of Pennisetum × advena ‘Rubrum’. In the study, temporary immersion system (TIS)- and agar-based methods were used in innovative workday organisation. The workday structure involved a six-hour passaging period followed by a two-hour break for medium preparation, autoclaving, and maintenance tasks. The TIS was found to be more cost-effective than the agar cultures, with lower labour costs and comparable growing expenses. The most expensive element of agar production was labour which was 43% of the costs. The second biggest cost was materials and reagents which represented 25%. In a TIS, production materials and reagents are the most expensive part of production (44%), while labour represents 24% of costs. A TIS offers a much faster multiplication of plants than agar cultures. Plants obtained in the multiplication phase are two times cheaper using a TIS. Rooting accounted for a significant portion of production costs in both methods. Overall, the TIS demonstrated superior efficiency and cost-effectiveness compared to agar cultures in producing Pennisetum × advena ‘Rubrum’ plants.

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Light Conditions and Auxin Influence the In Vitro Efficiency of Rooting and Acclimatization of Pennisetum ‘Vertigo®’

Prokopiuk,

Kapczyńska,

Pawłowska

2024

Agronomy

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This paper reports an efficient in vitro rooting and acclimatization method for Pennisetum ‘Vertigo®’. For that purpose, the influence of the IAA (indole-3-acetic acid) in MS (Murashige and Skoog) media, and different types of LED light (100% blue, 100% red, a combination of red and blue (70% + 30%), a combination of red, blue with yellow, green, or far-red (35% + 15% + 50%), and white LED) were investigated. A fluorescent lamp and a medium without auxin were used as controls. Subsequently, the plants were transferred ex vitro in trays to the greenhouse and later transplanted into pots. In all tested combinations, the shoots rooted at a high rate of 70–100% (except under blue light). The best results were obtained under white LED or the fluorescent lamp, where the highest number of roots was formed; however, IAA did not increase the rooting rate, the number, or the weight of roots. Shoots rooted under LEDs and on media containing IAA generally exhibited higher soluble sugar content compared to those rooted on auxin-free media. Consistent with the rooting results, the best acclimatization was observed in plants rooted under white LED or fluorescent light on auxin-free media.

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An Efficient Method of Pennisetum × advena ‘Rubrum’ Plantlets Production Using the Temporary Immersion Bioreactor Systems and Agar Cultures

Cited by 3 publications

References 46 publications

The Cytokinins BAP and 2-iP Modulate Different Molecular Mechanisms on Shoot Proliferation and Root Development in Lemongrass (Cymbopogon citratus)

The Cytokinins BAP and 2-iP Modulate Different Molecular Mechanisms on Shoot Proliferation and Root Development in Lemongrass (Cymbopogon citratus)

A Temporary Immersion System as a Tool for Lowering Planting Material Production Costs Using the Example of Pennisetum × advena ‘Rubrum’

Light Conditions and Auxin Influence the In Vitro Efficiency of Rooting and Acclimatization of Pennisetum ‘Vertigo®’

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