An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation

Pradhan, Bhola Shankar; Majumdar, Subeer S.

doi:10.1038/mtna.2016.9

Cited by 18 publications

(11 citation statements)

References 25 publications

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“…Primer details were given in Supplementary Table S1. The construct was linearized with Stu I , and was used to generate transgenic rat (Tg) as reported by us previously 47 . Briefly, the linearized construct (a total of 30 µg DNA with a concentration of 1 µg/µl) was injected into the testis of 38 days old Wistar rat, and electroporated using 8 square 90 V electric pulses in alternating direction with a time constant of 0.05 second and an inter-pulse interval of ~1 second via an electric pulse generator.…”

Section: Methodsmentioning

confidence: 99%

Downregulation of Sostdc1 in Testicular Sertoli Cells is Prerequisite for Onset of Robust Spermatogenesis at Puberty

Pradhan

Bhattacharya

Sarkar

et al. 2019

Sci Rep

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An alarming decline in sperm count of men from several countries has become a major concern for the world community. Hormones act on testicular Sertoli cells (Sc) to regulate male fertility by governing the division and differentiation of germ cells (Gc). However, there is a limited knowledge about Sc specific gene(s) regulating the spermatogenic output of the testis. Sclerostin domain-containing 1 protein ( Sostdc1 ) is a dual BMP/Wnt regulator is predominantly expressed in the Sc of infant testes which hardly show any sign of spermatogenesis. In order to investigate the role of Sostdc1 in spermatogenic regulation, we have generated transgenic (Tg) rats which induced persistent expression of Sostdc1 in mature Sc causing reduced sperm counts. Although Sc specific Sostdc1 did not affect the function of either Sc or Leydig cells (Lc) in the adult testis of Tg rat, we observed a selective augmentation of the BMP target genes via activated phospho smad 1/5/8 signaling in Gc leading to apoptosis. Here, for the first time, we have demonstrated that Sostdc1 is a negative regulator of spermatogenesis, and provided substantial evidence that down regulation of Sostdc1 during puberty is critically essential for quantitatively and qualitatively normal spermatogenesis governing male fertility.

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Section: Methodsmentioning

confidence: 99%

Downregulation of Sostdc1 in Testicular Sertoli Cells is Prerequisite for Onset of Robust Spermatogenesis at Puberty

Pradhan

Bhattacharya

Sarkar

et al. 2019

Sci Rep

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“…A large number of murine models are commercially available, and distributers can easily be found on the website of the “International Mouse Strain Resource” (IMSR, ). Even though transgenesis is more complicated in the rat than in the mouse, technical advances have recently been made and, as a result, the number of transgenic rat models for human disease will certainly significantly increase in the coming years [ 146 ].…”

Section: In Vivo Research Modelsmentioning

confidence: 99%

An Overview of In Vivo and In Vitro Models for Autosomal Dominant Polycystic Kidney Disease: A Journey from 3D-Cysts to Mini-Pigs

Koslowski

Latapy

Auvray

et al. 2020

IJMS

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Autosomal dominant polycystic kidney disease (ADPKD) is the most common inheritable cause of end stage renal disease and, as of today, only a single moderately effective treatment is available for patients. Even though ADPKD research has made huge progress over the last decades, the precise disease mechanisms remain elusive. However, a wide variety of cellular and animal models have been developed to decipher the pathophysiological mechanisms and related pathways underlying the disease. As none of these models perfectly recapitulates the complexity of the human disease, the aim of this review is to give an overview of the main tools currently available to ADPKD researchers, as well as their main advantages and limitations.

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“…Stable anaesthesia is generally easier to achieve, and appropriate monitoring of physiological parameters less invasive in rats due to their larger body size (compared to mice). As transgenic rats are becoming more accessible (Pradhan and Majumdar, 2016), implications of species selection on the expected data quality may become increasingly important for assessing the construct validity of a given experiment.…”

Section: Implications For Validity Of Bold Fmri In Rodentsmentioning

confidence: 99%

Systematic Review: Anaesthetic Protocols and Management as Confounders in Rodent Blood Oxygen Level Dependent Functional Magnetic Resonance Imaging (BOLD fMRI)–Part A: Effects of Changes in Physiological Parameters

et al. 2020

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Background: To understand brain function in health and disease, functional magnetic resonance imaging (fMRI) is widely used in rodent models. Because animals need to be immobilised for image acquisition, fMRI is commonly performed under anaesthesia. The choice of anaesthetic protocols and may affect fMRI readouts, either directly or via changing physiological balance, and thereby threaten the scientific validity of fMRI in rodents. Methods: The present study systematically reviewed the literature investigating the influence of different anaesthesia regimes and changes in physiological parameters as confounders of blood oxygen level dependent (BOLD) fMRI in rats and mice. Four databases were searched, studies selected according to pre-defined criteria, and risk of bias assessed for each study. Results are reported in two separate articles; this part of the review focuses on effects of changes in physiological parameters. Results: A total of 121 publications was included, of which 49 addressed effects of changes in physiological parameters. Risk of bias was high in all included studies. Blood oxygenation [arterial partial pressure of oxygen (p a O 2)], ventilation [arterial partial pressure of carbon dioxide (p a CO 2)] and arterial blood pressure affected BOLD fMRI readouts across various experimental paradigms. Conclusions: Blood oxygenation, ventilation and arterial blood pressure should be monitored and maintained at stable physiological levels throughout experiments. Appropriate anaesthetic management and monitoring are crucial to obtain scientifically valid, reproducible results from fMRI studies in rodent models.

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An Efficient Method for Generation of Transgenic Rats Avoiding Embryo Manipulation

Cited by 18 publications

References 25 publications

Downregulation of Sostdc1 in Testicular Sertoli Cells is Prerequisite for Onset of Robust Spermatogenesis at Puberty

Downregulation of Sostdc1 in Testicular Sertoli Cells is Prerequisite for Onset of Robust Spermatogenesis at Puberty

An Overview of In Vivo and In Vitro Models for Autosomal Dominant Polycystic Kidney Disease: A Journey from 3D-Cysts to Mini-Pigs

Systematic Review: Anaesthetic Protocols and Management as Confounders in Rodent Blood Oxygen Level Dependent Functional Magnetic Resonance Imaging (BOLD fMRI)–Part A: Effects of Changes in Physiological Parameters

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