An Efficient Method for Extraction of Astaxanthin from Green Alga <i>Haematococcus pluvialis</i>

Ravi, Saranya; Raman, Vidhyavathi; Dharmaraj, Usha; Ravishankar, G. A.

doi:10.1021/jf060737t

Cited by 196 publications

(131 citation statements)

References 15 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…Ash contents (mineral composition) in the algae are probably due to the concentration of inorganic compounds in the medium in which the algae grew, although ash content varied within each group. In fact, average ash content in this study was lowest than that found by Sarada et al (2006) with 4.6% dry weight for H. pluvialis.…”

Section: Discussioncontrasting

confidence: 81%

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

2013

View full text Add to dashboard Cite

Growth rate and medium parameters between two bench scale volumes (13-L and 250-L) were compared. Experiments were maintained batch mode and culture parameters were periodically measured during a 13-day period. Culture growth during the cultivation of algae Haematococcus pluvialis was determined qualitatively by cell counting, optical density, dry weight, ash content, amount of chlorophyll-a, total organic carbon content and by direct measuring of medium nutrients and some abiotic aspects. Vegetative cell growth was higher when cultured in 13-L with 1.33 x 10 5 cells.mL -1 on the 12 th day than when cultured in 250-L. Significant difference (p < 0.05) in the biology and water culture of H. pluvialis, with the exception of dry weight, ash, nitrite and ammonia, was reported between the volumes. Data obtained in current study for the upscale culture maintenance of H. pluvialis in laboratory conditions shows that it should be undertaken in a 13-L volume due to a greater time span of cells in a vegetative state, greater cell density, lipids and chlorophyll-a contents. Light was of paramount importance on the direct performance of H. pluvialis on the algal biological conditions. Keywords: biological aspects, growth, nutritional content, microalgae. Efeito de alguns parâmetros no cultivo em larga escala de Haematococcus pluvialis Flotow ResumoForam avaliadas a taxa de crescimento e condições do meio de cultura em volumes de 13-L e 250-L em sistema estático, durante o período de 13 dias para a microalga Haematococcus pluvialis. Foi determinada qualitativamente a contagem de células, densidade, peso seco, teor de cinzas, clorofila-a, teor de carbono orgânico total e avaliação de nutrientes e fatores abióticos do meio de cultura. O crescimento foi mais elevado em volume de 13-L com 1,33 x 10 5 células.mL -1 no décimo segundo dia, do que em volume de 250-L. Em relação ao meio de cultivo e aspectos biológicos de H. pluvialis, foram observadas diferenças significativas (p < 0,05) entre os dois volumes com exceção do peso seco, cinzas, nitrito e amônia. Para cultivo em larga escale de H. pluvialis é recomendado nestas condições laboratoriais, o volume de 13-L devido ao maior tempo das células em estado vegetativo, maior densidade celular e elevados teores de lipídios e clorofila-a. A luz interferiu diretamente nas condições biológicas de H. pluvialis. Palavras-chave: aspectos biológicos, crescimento, conteúdo nutricional, microalga.

show abstract

Section: Discussioncontrasting

confidence: 81%

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

2013

View full text Add to dashboard Cite

show abstract

“…The reported biomass productivities range from 0.03 to 0.6 g L pretreated by a mechanical means to disrupt the rigid cell walls, followed by a spray-drying process to produce dry biomass powder. If astaxanthin is the final product, a conventional solvent-based extraction method or supercritical fluid extraction can be applied to wet or dry Haematococcus biomass to obtain concentrated astaxanthin extracts (Bubrick 1991, Mendes-Pinto et al 2001, Nobre et al 2006, Sarada et al 2006, Krichnavaruk et al 2008). …”

Section: Mass Cultivation Of Haematococcus Pluvialis and Chlorella Zomentioning

confidence: 99%

Astaxanthin in microalgae: pathways, functions and biotechnological implications

Han¹,

Li²,

Hu³

2013

ALGAE

178

View full text Add to dashboard Cite

Major progress has been made in the past decade towards understanding of the biosynthesis of red carotenoid astaxanthin and its roles in stress response while exploiting microalgae-based astaxanthin as a potent antioxidant for human health and as a coloring agent for aquaculture applications. In this review, astaxanthin-producing green microalgae are briefly summarized with Haematococcus pluvialis and Chlorella zofingiensis recognized to be the most popular astaxanthin-producers. Two distinct pathways for astaxanthin synthesis along with associated cellular, physiological, and biochemical changes are elucidated using H. pluvialis and C. zofingiensis as the model systems. Interactions between astaxanthin biosynthesis and photosynthesis, fatty acid biosynthesis and enzymatic defense systems are described in the context of multiple lines of defense mechanisms working in concert against photooxidative stress. Major pros and cons of mass cultivation of H. pluvialis and C. zofingiensis in phototrophic, heterotrophic, and mixotrophic culture modes are analyzed. Recent progress in genetic engineering of plants and microalgae for astaxanthin production is presented. Future advancement in microalgal astaxanthin research will depend largely on genome sequencing of H. pluvialis and C. zofingiensis and genetic toolbox development. Continuous effort along the heterotrophic-phototrophic culture mode could lead to major expansion of the microalgal astaxanthin industry.

show abstract

“…The yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) and the microalgae Haematococcus pluvialis (H. pluvialis) are known as the main microorganisms capable of synthesizing astaxanthin 27 . A number of studies have been carried out to determine the best conditions to synthesize and extract astaxanthin from these microorganisms [28][29][30] . H. pluvialis accumulates higher amounts of ketocarotenoids in cytoplasmic lipid vesicles and has been reported to be the richest source of natural astaxanthin 14 , reaching 9.2mg/g cell 27 .…”

Section: Astaxanthin Sourcesmentioning

confidence: 99%

Astaxanthin: structural and functional aspects

Seabra

Pedrosa

2010

Rev. Nutr.

View full text Add to dashboard Cite

1 Article based on L.M.J. SEABRA' s thesis project entitled "Litopenaeus vannamei shrimp: components of nutritional importance in meat and processing waste. A B S T R A C TAstaxanthin, a carotenoid belonging to the xanthophyll class, has stirred great interest due to its antioxidant capacity and its possible role in reducing the risk of some diseases. Astaxanthin occurs naturally in microalgae, such as Haematococcus pluvialis and the yeast Phaffia rhodozyma, and has also been considered to be the major carotenoid in salmon and crustaceans. Shrimp processing waste, which is generally discarded, is also an important source of astaxanthin. The antioxidant activity of astaxanthin has been observed to modulate biological functions related to lipid peroxidation, having beneficial effects on chronic diseases such as cardiovascular disease, macular degeneration and cancer. Researches have shown that both astaxanthin obtained from natural sources and its synthetic counterpart produce satisfactory effects, but studies in humans are limited to natural sources. There is no established nutritional recommendation regarding astaxanthin daily intake but most studies reported beneficial results from a daily intake of 4mg. Thus, this review discusses some aspects of the carotenoid astaxanthin, highlighting its chemical structure and antioxidant activity, and some studies that report its use in humans.Indexing terms: Antioxidants. Astaxanthin. Carotenoids. Chronic diseases. R E S U M O

show abstract

An Efficient Method for Extraction of Astaxanthin from Green Alga Haematococcus pluvialis

Cited by 196 publications

References 15 publications

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

Effects of some parameters in upscale culture of Haematococcus pluvialis Flotow

Astaxanthin in microalgae: pathways, functions and biotechnological implications

Astaxanthin: structural and functional aspects

Contact Info

Product

Resources

About