An efficient, in vitro cyclic production of shoots from adult trees of Crataeva nurvala Buch. Ham.

Abstract

An efficient, cyclic, two-step protocol for micropropagation of medicinal tree, Crataeva nurvala has been successfully developed, which can be employed at a commercial scale. Nodal explants from 30-year-old tree when cultured on MS medium supplemented with 2.22 microM BAP produced multiple shoots, which elongated satisfactorily on the same medium. Nodal and leaf explants from in vitro regenerated microshoots too developed shoots, thus making the process recurrent. In 6-month duration, owing to the recurring nature of the protocol, over 5400 shoots could be produced from a single nodal explant from the adult tree. Addition of casein hydrolysate significantly increased the average number of shoots per explant. Maximum number of shoots regenerated on medium supplemented with 100 mg l(-1) casein hydrolysate. Shoots could be rooted on 1/2 MS supplemented with 0.11 and 0.54 microM NAA. Regenerated plantlets were acclimatized and successfully transplanted to soil.

Influence of Additives on Enhanced in Vitro Shoot Multiplication of <i>Stevia rebaudiana</i> (Bert.)—An Important Anti Diabetic Medicinal Plant

“…Similar results have also been found with CH in Anogeissus pendula, A. latifolia in vitro studies [30]. Induction of healthy shoot formation has been reported in Crataeva nurvala [31] using CH as medium supplement. Higher concentrations of casein hydrolysate (0.075% & 0.1%) resulted with no further increase in the number of shoots.…”

Effect of adenine sulphate, casein hydrolysate and spermidine on in vitro shoot multiplication of two banana varieties (FHIA-21 and PITA-3)

“…The number of shoots was found to be enhanced with 50 mg/L in FHIA-21 and 25 mg/L in PITA-3. Similar results have also been found with CH in Anogeissus pendula, Anogeissus latifolia (Saxena and Dhawan, 2001) and in Crataeva nurvala (Walia et al, 2007). Media components such as amino acids have demonstrated a profound effect on tissue culturing systems of several species (Benson, 2000;El-sharabasy et al, 2016).…”

“…Positive role of different growth additives such as adenine sulphate, casein hydrolysate and spermidine has been reported extensively in the in vitro propagation of several species (Walia et al, 2007;Sanghamitra and Satyabrata 2011;Siwach et al, 2012). Earlier, it was reported that spermidine is essential for shoot multiplication in cucumber (Vasudevan et al, 2008), sugarcane (Shankar et al, 2011) and Wrightia tomentosa (Joshi et al, 2014).…”

An efficient in vitro process for recurrent production of cloned plants of Vitex negundo L

“…A number of plant species have been investigated around the globe. Currently, enhanced axillary shoot proliferation from cultured meristem is the most frequently used method for micropropagation (Joshi and Dhawan 2007;Walia et al 2007; Makunga and van Staden 2008). It provides the genetic stability and is easily attainable for many plant species.…”