An efficientin vivo-inducible CRISPR interference system for group AStreptococcusgenetic analysis and pathogenesis studies
Elisabet Bjånes,
Alexandra Stream,
Axel B. Janssen
et al.
Abstract:While genome-wide transposon mutagenesis screens have identified numerous essential genes in the significant human pathogenStreptococcus pyogenes(group AStreptococcusor GAS), many of their functions remain elusive. This knowledge gap is attributed in part to the limited molecular toolbox for controlling GAS gene expression and the bacterium’s poor genetic transformability. CRISPR interference (CRISPRi), using catalytically inactive GAS Cas9 (dCas9), is a powerful approach to specifically repress gene expressio… Show more
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