An Efficient High Throughput Metabotyping Platform for Screening of Biomass Willows

Corol, Delia I.; Harflett, Claudia; Ward, Jane L.

doi:10.3390/metabo4040946

Cited by 17 publications

(21 citation statements)

References 59 publications

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“…Data from a standardised 1 H-NMR fingerprinting method for aqueous methanolic extracts of willow [ 11 ], that gives quantitative data on a mixture of primary and secondary metabolites, was mined to examine the variation in salicin concentration in stem tissue samples, across 86 pure (i.e non-hybrid) Salix genotypes in the NWC, harvested at the dormant stage (February), a time-point when biomass willows are generally cropped. Quantitative data derived from 1 H-NMR via integration of the distinctive and isolated benzylic hydrogens of salicin 1 (δ4.74 and δ4.69) against internal d 4 - trimethylsilylpropionate standard, are given in Table 1 .…”

Section: Resultsmentioning

confidence: 99%

“…For initial metabolite profiling by NMR and UHPLC-MS triplicate aliquots of milled freeze-dried willow stem powder (30 mg) were extracted as previously described [ 11 ]. Separate extractions were made for each analytical method.…”

Section: Methodsmentioning

confidence: 99%

“…As part of a programme dedicated to high value products from plants we have focussed on novel phytochemistry in the Salicaceae, in particular, those species contained in the 1500+ National Willow Collection (NWC), maintained as a short-rotation coppice plantation at Rothamsted Research. Taking a metabolomics approach [ 11 ] to polar extracts using NMR and high mass accuracy LC-MS-MS we have constructed a large annotated data-resource that spans the NWC and the full breadth of phenolic glycoside diversity. In this paper, we report on the discovery and structure determination of salicin-7-sulfate 3 ( Fig.…”

Section: Introductionmentioning

confidence: 99%

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Salicin-7-sulfate: A new salicinoid from willow and implications for herbal medicine

et al. 2018

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Willow (Salix sp.) is a historically well-known herbal medicine that provided the lead compound (salicin) for the discovery of aspirin, one of the most successful plant derived drugs in human medicine. During a metabolomics screen of 86 Salix species contained in the UK National Willow Collection, we have discovered, isolated and fully characterised a new natural salicinoid – salicin-7-sulfate. This molecule may have important human pharmacological actions that need to be considered in determining the efficacy and safety of willow herbal medicines.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Salicin-7-sulfate: A new salicinoid from willow and implications for herbal medicine

et al. 2018

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“…The 1D 1 H NMR data was collected on wheat leaf tissues according to procedures described by Ward et al (2010) and Corol et al (2014). Known metabolites present in the 1 H NMR spectra were quantified against an internal standard and compared using multivariate analysis.…”

Section: Methodsmentioning

confidence: 99%

Triticum monococcumlines with distinct metabolic phenotypes and phloem-based partial resistance to the bird cherry-oat aphidRhopalosiphum padi

et al. 2016

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Crop protection is an integral part of establishing food security, by protecting the yield potential of crops. Cereal aphids cause yield losses by direct damage and transmission of viruses. Some wild relatives of wheat show resistance to aphids but the mechanisms remain unresolved. In order to elucidate the location of the partial resistance to the bird cherry–oat aphid, Rhopalosiphum padi, in diploid wheat lines of Triticum monococcum, we conducted aphid performance studies using developmental bioassays and electrical penetration graphs, as well as metabolic profiling of partially resistant and susceptible lines. This demonstrated that the partial resistance is related to a delayed effect on the reproduction and development of R. padi. The observed partial resistance is phloem based and is shown by an increase in number of probes before the first phloem ingestion, a higher number and duration of salivation events without subsequent phloem feeding and a shorter time spent phloem feeding on plants with reduced susceptibility. Clear metabolic phenotypes separate partially resistant and susceptible lines, with the former having lower levels of the majority of primary metabolites, including total carbohydrates. A number of compounds were identified as being at different levels in the susceptible and partially resistant lines, with asparagine, octopamine and glycine betaine elevated in less susceptible lines without aphid infestation. In addition, two of those, asparagine and octopamine, as well as threonine, glutamine, succinate, trehalose, glycerol, guanosine and choline increased in response to infestation, accumulating in plant tissue localised close to aphid feeding after 24 h. There was no clear evidence of systemic plant response to aphid infestation.

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“…While comprehensive metabolomics relies on highly resolved and unbiased methods capable of distinguishing and quantifying speci c metabolites, these methods are not necessarily conducive to large sample set analysis in a reasonable timeframe, particularly if there are many metabolites present in the extracts. There have been a number of high-throughput metabolic screening methods developed for plant metabolomics, particularly involving the use of NMR (6) advanced mass spectrometry (7) and colorimetric (8) approaches to analyze extracts. Also, the rapid determination of biomass cell wall polymer content and composition has been achieved using minimal sample preparation followed by analysis using near-infrared spectroscopy or pyrolysis-Molecular Beam Mass Spectrometry (5).…”

Section: Introductionmentioning

confidence: 99%

Rapid screening of secondary aromatic metabolites in Populus trichocarpa leaves

Harman‐Ware

Martin

Engle

et al. 2020

Preprint

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Background High-throughput metabolomics analytical methodology is needed for population-scale studies of bioenergy-relevant feedstocks such as poplar (Populus sp.). Here, the authors report the relative abundance of secondary aromatic metabolites in Populus trichocarpa leaves rapidly estimated using pyrolysis-molecular beam mass spectrometry (py-MBMS). A training set of leaves was analyzed in conjunction with GC/MS analysis of extracts to determine key spectral features associated with the aromatic metabolites and build PLS models to predict the relative composition of extractable aromatic metabolites in a second test set of leaves. The relative abundance of major secondary aromatic metabolites for the test set of leaves was validated by GC/MS analysis of extracts. Results The Pearson correlation coefficient for the relative abundance of aromatic metabolites based on ranking between GC/MS analysis and py-MBMS analysis of the test leaf set was 0.86 with R2 = 0.76 using a simplified prediction approach from select ions in MBMS spectra. Metabolites most influential to py-MBMS spectral features in the training set included the following compounds: catechol, salicortin, salicyloyl-coumaroyl-glucoside, a-salicyloylsalicin, tremulacin, and to a lesser degree: benzyl-coumaroyl glucoside, benzoyl-salicyloylsalicin, tremuloidin, salireposide, trichocarpin, salicylic acid, salicyl-salicylic acid-2-O-glucoside, phenethyl-tremuloidin, coumaroyl-tremuloidin, 2,6-cyclohexadiene-1,2-diol, and salicyl-tremuloidin. Ions in py-MBMS spectra with the highest correlation to the abundance of aromatic secondary metabolites, particularly those previously mentioned as determined by GC/MS analysis of extracts, included m/z 68, 71, 77, 91, 94, 105, 107, 108, and 122, and were used to develop the simplified prediction approach without PLS models or a priori measurements. Conclusions The simplified py-MBMS method is capable of rapidly screening leaf tissue for relative abundance of extractable aromatic secondary metabolites to enable prioritization of samples in large populations requiring comprehensive metabolomics that will ultimately inform plant systems biology models and advance the development of optimized biomass feedstocks for renewable fuels and chemicals.

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An Efficient High Throughput Metabotyping Platform for Screening of Biomass Willows

Cited by 17 publications

References 59 publications

Salicin-7-sulfate: A new salicinoid from willow and implications for herbal medicine

Salicin-7-sulfate: A new salicinoid from willow and implications for herbal medicine

Triticum monococcumlines with distinct metabolic phenotypes and phloem-based partial resistance to the bird cherry-oat aphidRhopalosiphum padi

Rapid screening of secondary aromatic metabolites in Populus trichocarpa leaves

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