An efficient gene disruption system for the nematophagous fungus Purpureocillium lavendulum

Liu, Lu; Cao, Yan-Ru; Zhang, Chenchen; Fan, Hai-Feng; Guo, Zhengguang; Yang, Hongju; Mi, Chen; Han, Jingjing; Xu, Jianping; Zhang, Ke‐Qin; Liang, Lianming

doi:10.1016/j.funbio.2018.10.009

Cited by 17 publications

(23 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2A and B ). Our previous study showed that the PlbrlA mutant also had white colonies ( 8 ). Using a scanning electron microscope (SEM), it was observed that the ku80 KO strain developed normal conidiophores ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…They control the initiation and the middle and late stages of conidiogenesis. From our previous study, knocking out the PlbrlA gene in P. lavendulum totally blocked the formation of conidiophores ( 8 ). In the Δ PlbrlA mutant, the hyphae were extremely elongated, and the colony was loose and white.…”

Section: Discussionmentioning

confidence: 98%

“…Purpureocillium lavendulum wild-type strain YMF1.00683 was isolated from soil in Yunnan Province, China. The ku80 gene, involved in the nonhomologous end joining (NHEJ) pathway, was knocked out in this strain to create a deletion mutant which showed a high efficiency in homologous recombination ( 8 ). This deletion mutant was the main strain used for subsequent molecular analyses in this study.…”

Section: Methodsmentioning

confidence: 99%

“…This deletion mutant was the main strain used for subsequent molecular analyses in this study. Both the ku80 KO strain and its derived knockout mutant strains were maintained in minimal solid or liquid medium (MM) with 2% glucose and incubated at 28°C ( 8 ). Escherichia coli DH5α was used for vector construction.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Functional Characterization of Core Regulatory Genes Involved in Sporulation of the Nematophagous Fungus Purpureocillium lavendulum

Chen

Yang

Cao

et al. 2020

mSphere

Self Cite

View full text Add to dashboard Cite

The nematophagous fungus Purpureocillium lavendulum is a natural enemy of plant-parasitic nematodes, which cause severe economic losses in agriculture worldwide. The production of asexual spores (conidia) in P. lavendulum is crucial for its biocontrol activity against nematodes. In this study, we characterized the core regulatory genes involved in conidiation of P. lavendulum at the molecular level. The central regulatory pathway is composed of three genes, P. lavendulum brlA (PlbrlA), PlabaA, and PlwetA, which regulate the early, middle, and late stages of asexual development, respectively. The deletion of PlbrlA completely inhibited conidiation, with only conidiophore stalks produced. PlAbaA determines the differentiation of conidia from phialides. The deletion of PlwetA affected many phenotypes related to conidial maturation, including abscission of conidia from conidium strings, thickening of the cell wall layers, vacuole generation inside the cytoplasm, production of trehalose, tolerance to heat shock, etc. Comparative analyses showed that the upstream regulators of the core regulatory pathway of conidiation, especially the “fluffy” genes, were different from those in Aspergillus. Besides their roles in conidiation, the central regulators also influence the production of secondary metabolites, such as the leucinostatins, in P. lavendulum. Our study revealed a set of essential genes controlling conidiation in P. lavendulum and provided a framework for further molecular genetic studies on fungus-nematode interactions and for the biocontrol of plant-parasitic nematodes. IMPORTANCE Plant-parasitic nematodes cause serious damage to crops throughout the world. Purpureocillium lavendulum is a nematophagous fungus which is a natural enemy of nematodes and a potential biocontrol agent against plant-parasitic nematodes. The conidia play an important role during infection of nematodes. In this study, we identified and characterized genes involved in regulating asexual development of P. lavendulum. We found that these genes not only regulate conidiation but also influence secondary-metabolite production. This work provides a basis for future studies of fungus-nematode interactions and nematode biocontrol.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Functional Characterization of Core Regulatory Genes Involved in Sporulation of the Nematophagous Fungus Purpureocillium lavendulum

Chen

Yang

Cao

et al. 2020

mSphere

Self Cite

View full text Add to dashboard Cite

show abstract

“…The genus Purpureocillium was proposed in 2011 (Luangsa-Ard et al 2011) and P. lavendulum was proposed as a new species in 2013 (Perdomo et al 2013). We have created a high efficient genetic manipulation system for this fungus, thus facilitate the characterization of biological functions of nematode infection-related genes (Liu et al 2019). Based on the system, key regulators in conidiation procedure in P. lavendulum have been characterized (Chen et al 2020).…”

mentioning

confidence: 99%

Characterization of the complete mitochondrial genome of the nematophagous fungus Purpureocillium lavendulum

Liang

Zhang

et al. 2021

Mitochondrial DNA Part B

Self Cite

View full text Add to dashboard Cite

Eco-friendly management of Meloidogyne incognita in cadmium-contaminated soil by using nematophagous fungus Purpureocillium lavendulum YMF1.683: Efficacy and mechanism

Li,

Liang,

Hua

et al. 2024

Environmental Research

View full text Add to dashboard Cite

An efficient gene disruption system for the nematophagous fungus Purpureocillium lavendulum

Cited by 17 publications

References 26 publications

Functional Characterization of Core Regulatory Genes Involved in Sporulation of the Nematophagous Fungus Purpureocillium lavendulum

Functional Characterization of Core Regulatory Genes Involved in Sporulation of the Nematophagous Fungus Purpureocillium lavendulum

Characterization of the complete mitochondrial genome of the nematophagous fungus Purpureocillium lavendulum

Eco-friendly management of Meloidogyne incognita in cadmium-contaminated soil by using nematophagous fungus Purpureocillium lavendulum YMF1.683: Efficacy and mechanism

Contact Info

Product

Resources

About