An Efficient ex vivo Expansion of Adult Mesenchymal Stem Cells in Scaffolds

Park, Eui; Shin, Hong‐In; Kim, Shin‐Yoon

doi:10.1201/b11625-49

Cited by 1 publication

(1 citation statement)

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“…[ 22 ] SF can also be functionalized with different groups like carboxyl, hydroxyl, phenol, and amine. [ 23 ] Moreover, electrospun SF nanofibers have been fabricated as dressing material because of their rapid biodegradation. [ 21 ] The procedure involves the use of formic acid as a solvent after freeze‐drying the SF [ 24 ] and/or the use of an aqueous solvent containing a blend of sacrificial polymer, e.g., poly(ethylene oxide) in SF.…”

Section: Introductionmentioning

confidence: 99%

Hydrothermal Modification of As‐Spun Polyurethane Micro‐Nanofibers Using Silk Fibroin and Biologically Reduced Silver Nanoparticles for Efficient Hydrophilicity and Antibacterial Properties

Khan

Rather

Wani

et al. 2023

Advanced Sustainable Systems

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The hydrophobicity of polyurethane fibers is undesired for tissue engineering. In this work, fibers are fabricated by electrospinning and coated with silk fibroin (SF) for biocompatibility and biologically synthesized silver (Ag) nanoparticles (NPs) for antibacterial activity. The scanning electron microscopy revealed the undistorted morphology and the presence of NPs on fibers. The Fourier transform infrared and thermogravimetric analysis show the successful incorporation of SF and Ag NPs. The water contact angle drops from 101.26±0.2° in pristine to 3.1±0.3° in the hydrothermally treated fibers. The moisture regain from 1.87±0.3% to 6.7±0.4% is observed after 24 h. The mineralization shows the hydroxyapatite NPs in all composites. The large zone of inhibition is observed in the composite, with the Ag NPs equal to 11.5±0.04 mm for Staphylococcus aureus and 18.91±0.02 mm for Escherichia coli. The increased proliferation of HEK 293T cells for 6 days of culture on composite scaffolds compared to the pristine scaffolds is achieved. The cell attachment examinations show successful attachment of cells on fibers and nuclear staining analysis shows the highest proliferation in scaffolds with an intermediate concentration of Ag NPs (10%). This strategy presents a novel improvisation for as‐spun PU fibers that can fabricate wound‐healing biomaterials for tissue engineering.

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