An efficient electrochemical method for direct screening of the mutation status of DNA base in oligonucleotides

Wang, Po; Li, Xijiao; Dai, Zong; Zou, Xiaoyong; Han, Xiang

doi:10.1016/j.snb.2014.05.022

Cited by 12 publications

(2 citation statements)

References 55 publications

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“…In contrast, our method is based on commercially available screen‐printed electrodes and avoids surface functionalization step. Furthermore, the analytical performance of our method is either comparable or much better than many of the currently reported electrochemical methods for point mutations . For example, our method is much faster than the recently reported electrochemical detection of KRAS point mutation in pancreatic cancer samples .…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Detection of FAM134B Mutations in Oesophageal Cancer Based on DNA‐Gold Affinity Interactions

Haque

Islam

et al. 2017

Electroanalysis

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Inexpensive, simple and rapid DNA sensors capable of accurate and sensitive detection of cancer specific point mutations in DNA biomarkers are crucial for the routine screening of genetic mutations in cancer. Conventional approaches based on sequencing, mass spectroscopy, and fluorescence are highly effective, but they are tedious, slow and require labels and expensive equipment. Recent electrochemistry based approaches mostly rely on conventional DNA biosensing using recognition and transduction layers, and hence limited by the complicated steps of sensor fabrication associated with surface cleaning, self‐assembled monolayer formation, and target hybridization. Herein we report a relatively simple and inexpensive method for detecting point mutation in cancer by using the direct adsorption of purified DNA sequences onto an unmodified gold surface. The method relies on the base dependent affinity interaction of DNA with gold. Since the affinity interaction (adsorption) trend of DNA bases follows as adenine (A) > cytosine (C) > guanine (G)> thymine (T), two DNA sequences with different DNA base compositions (i. e., amplified mutated sequences will be distinctly different than its original sequence) will have different adsorption affinity towards gold. The amount of mutation sites on a DNA sequence is quantified by monitoring the electrochemical current as a function of the relative adsorption level of DNA samples onto a bare gold electrode. This method can successfully distinguish single point mutation in DNA from oesophageal cancer. We demonstrated the clinical utility of this approach by detecting different levels of mutations in tissue samples (n=9) taken from oesophageal cancer patients. Finally, the method was validated with High Resolution Melt (HRM) curve analysis and Sanger Sequencing.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Detection of FAM134B Mutations in Oesophageal Cancer Based on DNA‐Gold Affinity Interactions

Haque

Islam

et al. 2017

Electroanalysis

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“…The most important objective in DNA biosensors is to fabricate inexpensive, reproducible and rapid‐response devices for detection of DNA hybridization with high specificity and sensitivity. There are many studies based on spectrometry , surface plasmon resonance and electrochemical techniques which have been made to DNA determination. Electrochemical techniques bring some advantages such as simplicity, high speed, low cost, and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Label Free BRCA Hybridization by Single Use Antioxidant Modified Electrode

Muti

2017

Electroanalysis

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A novel DNA probe based on caffeic acid modified disposable pencil graphite electrodes were developed for the first time for the electrochemical determination of breast cancer gene sequence (BRCA) hybridization. Amino‐linked BRCA probe highly immobilized onto the caffeic acid modified electrode by means of the interaction between the amino group of BRCA probe and the carboxyl group of caffeic acid compared to the bare electrode. 44 % signal enhancement in guanine oxidation signal was measured by caffeic acid modified electrode. Besides, these probes exhibited high selectivity towards its complementary DNA sequences (target). Hybridization between probe and target (BRCA1) was studied to evaluate the selectivity of the probes for complementary, non‐complementary and mismatch sequences. The selectivity was also tested in the presence of mixture containing the target and one base mismatch BRCA sequences in the same ratio (1 : 1). It can be said this probe can select its complementary from the mixture.

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CdSe Quantum Dots Based Nano-Biosensor for Detection of 185delAG Mutation in BRCA1 Gene, Responsible for Breast Cancer

Eftekhari‐Sis

Karaminejad

Malekan

et al. 2017

J Inorg Organomet Polym

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An efficient electrochemical method for direct screening of the mutation status of DNA base in oligonucleotides

Cited by 12 publications

References 55 publications

Electrochemical Detection of FAM134B Mutations in Oesophageal Cancer Based on DNA‐Gold Affinity Interactions

Electrochemical Detection of FAM134B Mutations in Oesophageal Cancer Based on DNA‐Gold Affinity Interactions

Electrochemical Determination of Label Free BRCA Hybridization by Single Use Antioxidant Modified Electrode

CdSe Quantum Dots Based Nano-Biosensor for Detection of 185delAG Mutation in BRCA1 Gene, Responsible for Breast Cancer

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