An efficient DNA isolation protocol for  Cymbopogon species suitable for diverse downstream applications

Bishoyi, Ashok Kumar; Kavane, Aarti; Sharma, Avadhesh C.; Geetha, K.

doi:10.37855/jah.2016.v18i02.29

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…The collected leaves were ground to ne powder in mortar and pestle using liquid nitrogen. DNA extraction was carried out according to the modi ed CTAB protocol of Bishoyi et al (2016b). The quality of DNA was checked by performing on 0.8% agarose gel electrophoresis.…”

Section: Plant Materials Collectionmentioning

confidence: 99%

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

Patel

Purohit

Sapra

et al. 2022

Preprint

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Genetic diversity analysis contributes to the conservation, protection and utilization of genetic resources toward efficient management of germplasm. In this investigation, Inter Simple Sequence Repeats (ISSR) markers were used to analyze the genetic diversity of 32 genotypes of Acmella paniculata collected from 12 different states of Gujarat, India. There were 113 loci amplified by 15 different ISSR markers with an average of 7.53 loci per primer. From the total 113 amplicons; 81 were polymorphic, 43 were monomorphic and 9 were found to be unique showing an average of 60.18% polymorphism. The primer (CT)8T produced a high value (0.65) of polymorphic information content (PIC); whereas, primer (TC)8RA produced a low value (0.21). The resolving power (RP) value of the primers ranged from 5.25 in primer (TC)8RA to 23.19 in primer (AC)8YT with an average of 12.17. The Jaccard’s similarity coefficients value ranged from 0.48 to 0.94. The highest percentage of similarity (94%) was found between accessions AP6 and AP16 which were collected from Panchmahal and Narmada respectively, as they are geographically closely located. Whereas, accessions AP2 from Banaskantha and AP7 from Panchmahal were most distantly related with the similarity value of 48%, as expected to their collection locations. The study revealed that there is no correlation between geographical distance and genetic diversity among the populations studied. The resultant data generated from ISSR molecular marker-based genetic diversity analysis of A. paniculata in Gujarat can provide a reference for the conservation and efficient management of the important Indian genetic resources.

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Section: Plant Materials Collectionmentioning

confidence: 99%

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

Patel

Purohit

Sapra

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

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DNA isolation of clove and lemongrass using modification of the Doyle and Doyle methods and their relation with antioxidant activity

Listyanto

Muhammad

Erfianti

et al. 2021

J. Phys.: Conf. Ser.

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Clove (Syzigium aromaticum) and lemongrass (Cymbopogon winterianus) are essential oil-producing plants that are widely available in Indonesia and have high economic value for medicinal and industrial needs because of their antioxidant activity. However, the information about the relationship between antioxidant activity and the DNA content in these plants was very limited. Especially because DNA isolation of aromatic plants is also not easy to do with methods that are widely used. Therefore, this study aims to isolate DNA from clove and lemongrass using a modified Doyle and Doyle method and to find the relationship between the quality and quantity of DNA with their antioxidant activity. The method used was the use of PVP, isopropanol and sodium acetate for the modification of the Doyle and Doyle method in DNA isolation followed by antioxidant analysis using DPPH. The results showed that the modification to the Doyle and Doyle method had obtained high concentrations of DNA. Besides that, the clove DNA content is higher than lemongrass which is supported by the antioxidant activity of both plants.

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An efficient DNA isolation protocol for Cymbopogon species suitable for diverse downstream applications

Cited by 2 publications

References 7 publications

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

Genetic Diversity Analysis Revealed the Hot Spot of Acmella Paniculata Existing in Natural Populations of Gujarat

DNA isolation of clove and lemongrass using modification of the Doyle and Doyle methods and their relation with antioxidant activity

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