An efficient and reliable DNA extraction method for preimplantation genetic diagnosis: a comparison of allele drop out and amplification rates using different single cell lysis methods

Kim, Sung Ah; Yoon, Jung Ah; Kang, Moon Joo; Choi, Young Min; Chae, Suchan; Moon, Shin Yong

doi:10.1016/j.fertnstert.2008.06.033

Cited by 14 publications

(11 citation statements)

References 25 publications

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“…Fortunately, in view of the disadvantages in MDA, methods to improve the accuracy of the techniques used will reduce the likelihood of these disadvantages. Several improvements have been suggested to overcome the ADO problem, such as the use of alkaline lysis buffer in order to break down the cell and make the DNA more accessible, and the use of lysis buffer containing proteinase and detergent as an effective way to help reduce ADO (Thornhill et al, 2001;Kim et al, 2009). Linker-adaptor amplification was also suggested to lower ADO rates .…”

Section: Preferential Amplification and Allele Dropoutmentioning

confidence: 99%

Whole genome amplification in preimplantation genetic diagnosis

Zheng

Wang

et al. 2011

J. Zhejiang Univ. Sci. B

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Preimplantation genetic diagnosis (PGD) refers to a procedure for genetically analyzing embryos prior to implantation, improving the chance of conception for patients at high risk of transmitting specific inherited disorders. This method has been widely used for a large number of genetic disorders since the first successful application in the early 1990s. Polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) are the two main methods in PGD, but there are some inevitable shortcomings limiting the scope of genetic diagnosis. Fortunately, different whole genome amplification (WGA) techniques have been developed to overcome these problems. Sufficient DNA can be amplified and multiple tasks which need abundant DNA can be performed. Moreover, WGA products can be analyzed as a template for multi-loci and multi-gene during the subsequent DNA analysis. In this review, we will focus on the currently available WGA techniques and their applications, as well as the new technical trends from WGA products.

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Section: Preferential Amplification and Allele Dropoutmentioning

confidence: 99%

Whole genome amplification in preimplantation genetic diagnosis

Zheng

Wang

et al. 2011

J. Zhejiang Univ. Sci. B

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“…The accurate amplification of a specific region is the most important criterion in the clinical use of PGD. [24][25][26] In conclusion, it is suggested that the hot start PCR method may be a useful method for PGD-PCR that could improve the success of diagnosis. But this data have obtained only two families.…”

Section: Discussionmentioning

confidence: 99%

Application of Hot Start PCR Method in PCR-based Preimplantation Genetic Diagnosis

et al. 2012

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“…Reports also exist in relation to the credibility and precision of these diagnostic methods [2,[9][10][11][12][13][14][21][22][23]. We further evaluated the AF and ADO rates in a large population of single cells obtained from individuals heterozygous for a mutation in one of the three genes, HEXA, IKBKAP and ASPA.…”

Section: Discussionmentioning

confidence: 99%

“…ADO might potentially lead to the transfer of an affected embryo and consequently to misdiagnosis. Several hypotheses as to the origin of ADO and techniques to minimize it have been proposed [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%