An efficient and rapid in vitro regeneration system for metal resistant cotton

Bıçakçı, Emre; Memon, Abdul Razaque

doi:10.1007/s10535-005-0018-5

Cited by 6 publications

(2 citation statements)

References 8 publications

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“…Cotton micropropagation in vitro has been successful in a number of cultivars (Sulekha et al 2000;Jin et al 2006;Sun et al 2006;Tanreer et al 2006). Micropropagation protocols have been developed to use different explant sources, such as shoot apex (Aydin et al 2004;Ozyigit and Gozukirmizi 2009), hypocotyl (Aydin et al 2004;Jin et al 2006;Sun et al 2009), and cotyledonary node (Sulekha et al 2000;Bicakci and Memon 2005); the types of media used can vary in composition, using different concentrations and kinds of plant growth regulators (Zhu and Sun 2000;Mithilesh and Bakesh 2001;Zhang et al 2001;Sakhanokho et al 2005;Sujatha and Sailaja 2005;Zhao et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro

Tang

2010

Plant Cell Tiss Organ Cult

217

118

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The objective of this study was to determine the effects of different light-emitting diode (LED) light sources on the growth of upland cotton (Gossypium hirsutum L.) plantlets. Shoot bud apex cuttings of upland cotton (1.0 cm) were transplanted on Murashige and Skoog (MS) basal medium supplemented with 0.1 mg/l 6-benzyladenine (BA) and 0.5 mg/l naphthalene acetic acid (NAA) and cultured in vitro for 45 days. They were exposed to 50 lmol m -2 s -1 photosynthetic photon flux (PPF) and a 12-h photoperiod under six different lights: fluorescent lamp (CON), monochromatic blue LED (B), three blue and red LED mixtures (B:R = 3:1, 1:1, 1:3) and monochromatic red LED (R). The effects of the six light sources on growth and morphogenesis of upland cotton plantlets grown in vitro were investigated. Fresh weight, dry weight, stem length and second internode length were greatest in plantlets cultured under the B:R = 1:1 blue and red LED light, followed by blue LED light, and they were lowest in plantlets cultured under a fluorescent lamp. Chlorophyll content, leaf thickness, palisade tissue length, leaf and stomata area were highest in plantlets cultured under blue LED light. Root activity, sucrose, starch and soluble sugar contents were highest in plantlets cultured under red LED light. Our results showed that larger, healthier plantlets and a greater biomass of upland cotton were produced in the presence of red LED supplemented with a quantity of blue LED light. Blue and red LED (B:R = 1:1) was the most suitable light for the growth of upland cotton plantlets in vitro, and it may be used as alternative light source for an upland cotton culture system.

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Section: Introductionmentioning

confidence: 99%

Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro

Tang

2010

Plant Cell Tiss Organ Cult

217

118

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“…Olgun 'Station' tohumları, Bıçakcı ve Memon (2005) yöntemine göre, %5 sodyum hipoklorit ile 6 dakika sterilize edildikten sonra steril distile su ile 5 dakika boyunca durulanmış ve bu işlem üç kez tekrarlanmıştır (Bicakci ve Memon 2005). Tohumlar steril filtre kağıdı ile kurutulmuş, ardından %3 sakkaroz ve %0,6 agar ile takviye edilmiş bazal Murasige ve Skoog (Murashige ve Skoog 1962) ortamında inkübe edilmiştir.…”

Section: Kültür Ortamlarının Hazırlanması Ve Sterilizasyonuunclassified

Metal Akümülatör Bitki olan Brassica nigra L.’nin in vitro Kallus İndüksiyonu ve Sürgün Gelişimi

Memon

2021

Turkish JAF Sci.Tech.

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This study established an efficient in vitro callus formation and plant regeneration protocol for a prevıously reported Cu accumulator, Brassica nigra, black mustard collected from Diyarbakir (Station site). Node explants from 10-day old mature plants were used for callus formation and shoot regeneration. The highest callus formation frequency (100%) was observed on Murasige Skoog (MS) medium containing 0.1 mg/L Benzylaminopurine (BAP) + 0.5 mg/L Naphthylacetic acid (NAA) (MS 2), 0.6 mg/L BAP + 0.2 mg/L NAA (MS 7), the highest shoot regeneration frequency (100%) was achieved on MS medium containing 0.6 mg/L BAP + 0.05 mg/L Indole butyric acid (IBA) (MS 8), 0.2 mg/L IBA + 0.2 mg/L NAA (MS 10) and the highest number of shoots per explant (3,25) was obtained on MS medium supplemented 0.6 mg/L BAP + 0.05 mg/L IBA (MS 8). After root, stem, and leaf formation from explants in MS medium, these plants were transferred to soil and grown in the plant growth room for one month. A dependable and effective shoot regeneration procedure was developed, laying the groundwork for genetic transformation in Brassica nigra.

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Stress response of NAD+-dependent formate dehydrogenase in Gossypium hirsutum L. grown under copper toxicity

Gur

DeMi̇rci̇

Sunulu

et al. 2018

Environ Sci Pollut Res

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Cotton (Gossypium hirsutum L.), which is not directly involved in the food chain, appears to be a suitable candidate to remove heavy metals from the food chain and to be a commercial plant which could be planted in contaminated soils. The key point of this approach is selection of the right genotype, which has heavy metal resistance or hyperaccumulation properties. Therefore, in the present study, two G. hirsutum genotypes, Erşan-92 and N-84S, were grown under copper stress and investigated to obtain further insights about the heavy metal tolerance mechanisms of plants by focusing on the expression of NAD-dependent formate dehydrogenase (FDH). In accordance with the results, which were obtained from RT-PCR analysis and activity measurements, in the Erşan-92 root tissue, FDH activity increased significantly with increasing metal concentrations and a 6.35-fold higher FDH activity was observed in the presence of 100-μM Cu. As opposed to Erşan-92, the maximum FDH activity in the roots of N-84S, which were untreated with copper as the control plants, was measured as 0.0141-U mg g FW, and the activity decreased significantly with the increasing metal concentrations. The metallothionein (GhMT3a) transcript level of the plants grown in a medium containing different Cu concentrations showed nearly the same pattern as that of the FDH gene transcription. It was observed that while the tolerance of N-84S in the lower Cu concentration reduces remarkably, Erşan-92 continues to struggle up to 100-μM Cu. The results of the SOD analysis also confirm this activity of Erşan-92 against the Cu stress.

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An efficient and rapid in vitro regeneration system for metal resistant cotton

Cited by 6 publications

References 8 publications

Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro

Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro

Metal Akümülatör Bitki olan Brassica nigra L.’nin in vitro Kallus İndüksiyonu ve Sürgün Gelişimi

Stress response of NAD+-dependent formate dehydrogenase in Gossypium hirsutum L. grown under copper toxicity

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