An efficient amperometric transketolase assay: Towards inhibitor screening

Touisni, Nadia; Charmantray, Franck; Hélaine, Virgil; Hecquet, Laurence; Mousty, Christine

doi:10.1016/j.bios.2014.06.019

Cited by 10 publications

(18 citation statements)

References 35 publications

(43 reference statements)

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“…(1) and ( 2)]. [19,20] Under these conditions, the oxidation peak attributed to the oxidation of Prussian white (PW) into Prussian blue is observed at + 0.2 Vv s. Ag/ AgCl, and the reverser eactioni sf ound at ap otential of + 0.04 Vv s. Ag/AgCl, in accordance with the literature. [28] In the presence of 1mm H 2 O 2 (Figure2a), the cathodic current increases, which thus confirmst he oxidation of PW by H 2 O 2 .…”

Section: Electrochemical Characterization Of Electrodessupporting

confidence: 86%

“…This buffer is also suitable for the electrochemical detection of the activity of TK, and KCl is necessary for the electrochemical reduction of Prussian blue [Eqs. (1) and (2)] . Under these conditions, the oxidation peak attributed to the oxidation of Prussian white (PW) into Prussian blue is observed at +0.2 V vs. Ag/AgCl, and the reverse reaction is found at a potential of +0.04 V vs. Ag/AgCl, in accordance with the literature .…”

Section: Resultsmentioning

confidence: 99%

“…After having determined the characteristics of the electrodes, this device was used to measure H 2 O 2 produced by GAOx by using IPA in the presence of two of its substrates: dihydroxyacetone (DHA), the best GAOx substrate described in the literature, and l ‐erythrulose ( l ‐ery), a product of the reaction of TK that can be used to determine TK activity …”

Section: Resultsmentioning

confidence: 99%

“…[10] GAOx has been used since the 1990s in electrochemical biosensors for the detection of galactose, [11,12] lactose (together with a b-galactosidase), [13] dihydroxyacetone (DHA), [14][15][16] and, to al esser extent, superoxide radicalsa nd histidine (in the presence of ascorbic acid). [17,18] More recently,i tw as used for the detection of transketolase( TK) activity,a ne nzyme involved in different pathologies: [19,20] transketolase reactionp roducts can be specifically oxidized by GAOx;t his leads to the coproductiono fH 2 O 2 ,w hich is detected by amperometry.…”

Section: Introductionmentioning

confidence: 99%

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High‐Throughput Electrochemical Screening Assay for Free and Immobilized Oxidases: Electrochemiluminescence and Intermittent Pulse Amperometry

Aymard

Bonaventura²,

Henkens³

et al. 2016

ChemElectroChem

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Analysis of a large number of samples by electrochemical methods for rapid optimization of amperometric biosensors or screening applications is still a challenge. To overcome this limitation, a system with 96 screen‐printed electrodes was developed to assay H2O2 produced by free and immobilized galactose oxidase as a model for oxidases. Detection was based on two electrochemical methods: intermittent pulse amperometry (IPA) and electrochemiluminescence (ECL). This 96‐well electrochemical device allowed rapid optimization of the parameters in half a day, regardless of the method used (i.e., IPA or ECL). We demonstrate that ECL is more sensitive for the detection of H2O2 (limit of detection of 1 μm for ECL vs. 10 μm for IPA) with lower interelectrode variability (1 vs. 19 %). Rapid determination of sensitivities, linear ranges, and limits of detection of galactose oxidase substrates (e.g., dihydroxyacetone and l‐erythrulose) by using free or immobilized enzyme was conducted in only 50 min by the mean of 5 different sets of 96 screen‐printed electrodes.

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Section: Electrochemical Characterization Of Electrodessupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High‐Throughput Electrochemical Screening Assay for Free and Immobilized Oxidases: Electrochemiluminescence and Intermittent Pulse Amperometry

Aymard

Bonaventura²,

Henkens³

et al. 2016

ChemElectroChem

Self Cite

View full text Add to dashboard Cite

show abstract

“…An analog of thiamine, 5a ['N3'-pyridyl thiamine'; 3-(6-methyl-2-amino-pyridin-3-ylmethyl)-5-(2-hydroxy-ethyl)-4-methylthiazol-3-ium chloride hydrochloride] can also inhibit TKT [108] and ring-opened analogs of 5a improve its pharmacokinetic profile and show better effect in vivo [109]. Consequently, a bienzymatic biosensor was designed to screen various human TKT inhibitors [110]. With the knockdown of TKT and G6PD in A549 cell xenograft mouse model, tumor volume was dramatically repressed, and the same phenomena was observed when Nrf2 was knocked down [79].…”

Section: Targeting Anabolic Processmentioning

confidence: 99%

The greedy nature of mutant RAS: a boon for drug discovery targeting cancer metabolism?

Lv¹,

Wang²,

Chang³

et al. 2016

ABBS

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RAS oncogene mutations are frequently detected in human cancers. Among RAS-mediated tumorigenesis, KRAS-driven cancers are the most frequently diagnosed and resistant to current therapies. Despite more than three decades of intensive efforts, there are still no specific therapies for mutant RAS proteins. While trying to block those well-established downstream pathways, such as the RAF-MAPK pathway and the PI3K-AKT pathway, attentions have been paid to potential effects of RAS on metabolic pathways and the feasibility for targeting these pathways. Recent studies have proved that RAS not only promotes aerobic glycolysis and glutamine metabolism reprograming to provide energy, but it also facilitates branched metabolism pathways, autophagy, and macropinocytosis. These alterations generate building blocks for tumor growth and strengthen antioxidant defense in tumor cells. All of these metabolic changes meet different demands of RAS-driven cancers, making them distinct from normal cells. Indeed, some achievements have been made to inhibit tumor growth through targeting specific metabolism rewiring in preclinical models. Although there is still a long way to elucidate the landscape of altered metabolism, we believe that specific metabolic enzymes or pathways could be therapeutically targeted for selective inhibition of RAS-driven cancers.

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Tailoring Hybrid Layered Double Hydroxides for the Development of Innovative Applications

Taviot-Guého

Prévot

Forano

et al. 2017

Adv Funct Materials

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232

142

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International audienceHybrid materials based on layered double hydroxides (LDHs) exhibit great potential in diverse fields such as health care, polymer composites, environment, catalysis, and energy generation. Indeed, the compositional flexibility and the scalability of LDH structures, their low cost, and their ease of synthesis have made hybrid LDHs extremely attractive for constructing smart and high-performance multifunctional materials. This review provides a comprehensive and critical overview of the current research on multifunctional hybrid LDHs. Organic–inorganic hybrid LDHs, intercalated and surface-immobilized structures, are both specifically addressed. The new trends and strategies for hybrid LDH synthesis are first described, and then the potential of the latest hybrid LDHs, polymer LDH nanocomposites, and LDH bio-nanocomposites are presented. Significant achievements published from ≈2010, including authors' results, which employ hybrid LDH assemblies in materials science, medicine, polymer nanocomposites, cement chemistry, and environmental technologies, are specifically addressed. It is concluded with remarks on present challenges and future prospects

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An efficient amperometric transketolase assay: Towards inhibitor screening

Cited by 10 publications

References 35 publications

High‐Throughput Electrochemical Screening Assay for Free and Immobilized Oxidases: Electrochemiluminescence and Intermittent Pulse Amperometry

High‐Throughput Electrochemical Screening Assay for Free and Immobilized Oxidases: Electrochemiluminescence and Intermittent Pulse Amperometry

The greedy nature of mutant RAS: a boon for drug discovery targeting cancer metabolism?

Tailoring Hybrid Layered Double Hydroxides for the Development of Innovative Applications

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