“…Because the HUPE 114158 strain did not grow properly in YPD, the BHI medium was used with the same concentration of hygromycin B. For the selective purpose, yeast nitrogen base (YNB) solid medium (0.16% BD Difco YNB, 2% glucose, 0.5% ammonium sulfate, 25 μg/mL of leucine, 25 μg/mL of histidine, 25 μg/mL of methionine, and 25 μg/mL of uracil), supplemented with Chlorimuron ethyl (30 μg/mL, Santa Cruz Biotechnology, USA) was used for selection of S. brasiliensis transformed with A. tumefaciens carrying the plasmid described elsewhere ( 22 ).…”