An effective pre-treatment method for eliminating interference by serum albumin for analysis of anti-rHSA antibodies

Sun, Yunjuan; Xu, Jingzhi; Xie, Xinyao; Song, Haifeng

doi:10.1039/d2ay01528e

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…The removal of serum also eliminates potential interference with the compound's activity. This approach aids in accurately assessing the direct impact of the compound on the cells, facilitating a clearer interpretation of the assay results [76,77].…”

Section: In Vitro Cellular Assaymentioning

confidence: 99%

Cannabidiol-Loaded Solid Lipid Nanoparticles Ameliorate the Inhibition of Proinflammatory Cytokines and Free Radicals in an In Vitro Inflammation-Induced Cell Model

Alcantara,

Malabanan,

Nalinratana

et al. 2024

IJMS

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Cannabidiol (CBD) is a non-psychoactive compound derived from Cannabis sativa. It has demonstrated promising effects in combating inflammation and holds potential as a treatment for the progression of chronic inflammation. However, the clinical application of CBD is limited due to its poor solubility and bioavailability. This study introduces an effective method for preparing CBD-loaded solid lipid nanoparticles (CBD-SLNs) using a combination of low-energy hot homogenization and ultrasonication. We enhanced this process by employing statistical optimization with response surface methodology (RSM). The optimized CBD-SLN formulation utilizes glyceryl monostearate as the primary lipid component of the nanocarrier. The CBD-SLN formulation is screened as a potential tool for managing chronic inflammation. Stable, uniformly dispersed spherical nanoparticles with a size of 123 nm, a surface charge of –32.1 mV, an encapsulation efficiency of 95.16%, and a drug loading of 2.36% were obtained. The CBD-SLNs exhibited sustained release properties, ensuring prolonged and controlled CBD delivery, which could potentially amplify its therapeutic effects. Additionally, we observed that CBD-SLNs significantly reduced both reactive oxygen and nitrogen species and proinflammatory cytokines in chondrocyte and macrophage cell lines, with these inhibitory effects being more pronounced than those of free CBD. In conclusion, CBD-SLNs demonstrated superiority over free CBD, highlighting its potential as an effective delivery system for CBD.

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Section: In Vitro Cellular Assaymentioning

confidence: 99%

Cannabidiol-Loaded Solid Lipid Nanoparticles Ameliorate the Inhibition of Proinflammatory Cytokines and Free Radicals in an In Vitro Inflammation-Induced Cell Model

Alcantara,

Malabanan,

Nalinratana

et al. 2024

IJMS

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show abstract

Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer

Tejerina-Miranda,

Blázquez-García,

Serafín

et al. 2023

International Journal of Biological Macromolecules

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Selective Enrichment and Quantification of Honokiol in Human Plasma by Dispersive Solidphase Extraction with Fluorescence Detection

Tang,

Liu,

Liu

2023

Preprint

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An effective pre-treatment method for eliminating interference by serum albumin for analysis of anti-rHSA antibodies

Abstract: Analysis of anti-drug antibodies (ADAs) is important for risk assessment in clinical trials. ADA detection can be very difficult in the presence of high circulating levels of drug or target...

Cited by 3 publications

References 30 publications

Cannabidiol-Loaded Solid Lipid Nanoparticles Ameliorate the Inhibition of Proinflammatory Cytokines and Free Radicals in an In Vitro Inflammation-Induced Cell Model

Cannabidiol-Loaded Solid Lipid Nanoparticles Ameliorate the Inhibition of Proinflammatory Cytokines and Free Radicals in an In Vitro Inflammation-Induced Cell Model

Electrochemical biotool for the dual determination of epithelial mucins associated to prognosis and minimal residual disease in colorectal cancer

Selective Enrichment and Quantification of Honokiol in Human Plasma by Dispersive Solidphase Extraction with Fluorescence Detection

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