An Effective Micropropagation System Using Embryogenic Calli Induced from Bud Clusters in Asparagus officinalis L.

Kohmura, Hiroyuki; Chokyu, S.; Harada, Takashi

doi:10.2503/jjshs.63.51

Cited by 18 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, for NAA + KN there was lower percentage of response. Present findings corroborate with the findings of Gyulai et al, (1992), Kohmura et al, (1994), Mustapha et al, (1996), Sharma et al, (2011), Dezfuli et al, (2013), Patel and Patel (2015), Azad et al, (2017), Pank andJoshi (2017), Lamor et al, (2018), and Chaudhary and Dantu (2019). These workers induced calli for morphogenetic studies.…”

Section: Discussionsupporting

confidence: 90%

TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

Kumari¹,

Kumari²,

Singh³

2020

Indian Journal of Scientific Research

View full text Add to dashboard Cite

Tissue culture study was performed to standardize the technique and select suitable plant growth regulators and their concentrations for efficient callus induction from the nodal explants of Asparagus racemosus willd, an important medicinal herb. Different concentrations of NAA, 2,4-D, BAP and KN were supplemented in MS basal medium in which the nodal explants were inoculated. It was observed that MS + 0.2 mg/l NAA was the best culture condition in which maximum percentage of response 90.36 was obtained. This was followed by the culture conditions that were MS+ 2.0 mg/l where the percentage response was 88.68%. Similarly, next higher percentage 88.62 was observed in MS+1.0 mg/l BAP + 1.0 mg/l NAA. It was further observed that growth rate was excellent where the percentage response was higher. Second it was noted that the calli had both compact and friable texture and their colour varied from green yellow to brown even where the growth was excellent. It was further noted that NAA or BAP had no response for callusing when used alone. However, KN at higher concentration 2.0 mg/l gave the highest percentage of response for callusing. NAA + BAP and NAA + KN at the similar concentrations gave different result. In the MS medium having 1.0 mg/l BAP + 1.0 mg/l NAA the percentage of response was 88.62 while MS + 1.0 mg/l KN + 1.0 mg/l NAA the percentage of response was 62.34 only. Growth rate of the calli also differed in different culture conditions.

show abstract

Section: Discussionsupporting

confidence: 90%

TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

Kumari¹,

Kumari²,

Singh³

2020

Indian Journal of Scientific Research

View full text Add to dashboard Cite

show abstract

“…Adventitious regeneration from callus (Kar and Sen, 1985) and clonal propagation through axillary branching (Bopana and Saxena, 2008) have been reported earlier in A. racemosus. In vitro proliferation in this genus through somatic embryos has been reported for Asparagus species such as A. officinalis (Kohmura et al 1994;Li and Wolyn, 1995) A. breslerianus (Mousavizadeh et al 2017) through this process has been reported for a large number of other species such as Carica papaya L. (Anandan et al 2012), Musa (Ma et al 2012;Divakaran and Nair, 2011), and Cymbidium bicolor Lindl. (Mahendran and Bai, 2012).…”

Section: Introductionmentioning

confidence: 92%

Induction of somatic embryos in cultures of Asparagus racemosus Willd: an endangered medicinally important plant

Chaudhary

Dantu

2019

Bull Natl Res Cent

View full text Add to dashboard Cite

Background: Somatic embryogenesis is one of the most popular in vitro regeneration methods for mass micropropagation. In the present study, somatic embryogenesis via zygotic embryos was studied in Asparagus racemosus Willd. Since the callus quality plays an important role in plantlet development, therefore, compact embryogenic callus was selected for further embryogenesis. Results: Somatic embryos were induced by zygotic embryos germinating on callus induction medium (MS media with 1.54 mg L −1 2,4-D and 0.43 mg L −1 kinetin) in dark. Thereafter, the compact embryogenic callus differentiated up on MS media with 0.1 mg L −1 NAA and 0.5 mg L −1 kinetin supplemented with various concentrations of ancymidol. An addition of 0.75 mg L −1 ancymidol resulted in significant enhancement of somatic embryo formation and no malformed embryos were observed. Scanning electron micrographs and thin sections confirmed the structures as somatic embryos. Furthermore, these embryos were transferred to the same medium supplemented with glutamine, casein hydrolysate, and 3% sucrose for conversion into green shoots. These shoots could be multiplied in vitro using BAP-supplemented medium. Conclusions: An effective method for conservation of an overexploited threatened medicinally important species Asparagus racemosus has been developed. This is the first report of the formation of somatic embryos from zygotic embryos in A. racemosus. Ancymidol in combination with kinetin and NAA was found to be most efficient for somatic embryo maturation and germination. The established protocol would certainly advance the efficient somatic embryo induction, maturation, and germination which could be utilized for large-scale propagation of Asparagus species.

show abstract

“…However, not all of the genotypes showed the ability to induce embryogenic calli. Delbreil and Jullien [23] described that differences were observed for the frequency of embryogenic callus induction from shoot for micropropagation [15][16][17]24]. However, several reports showed superior effects of plant growth regulators for enhancing somatic embryogenesis.…”

Section: Genotypementioning

confidence: 99%

Somatic Embryogenesis and Its Application for Breeding and Micropropagation in Asparagus (Asparagus officinalis L.).

Kunitake¹,

Mii

1998

Plant Biotechnology

View full text Add to dashboard Cite

An Effective Micropropagation System Using Embryogenic Calli Induced from Bud Clusters in Asparagus officinalis L.

Cited by 18 publications

References 13 publications

TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

TISSUE CULTURE STUDY FOR EFFICIENT CALLUS INDUCTION FROM INTERNODAL EXPLANTS OF Asparagus racemosus WILLD AN IMPORTANT MEDICINAL HERB

Induction of somatic embryos in cultures of Asparagus racemosus Willd: an endangered medicinally important plant

Somatic Embryogenesis and Its Application for Breeding and Micropropagation in Asparagus (Asparagus officinalis L.).

Contact Info

Product

Resources

About