An effective indexing method for banana tissue culture provides long-term freedom from bacterial contamination

Hamill, S. D.; Rames, E.

doi:10.17660/actahortic.2018.1205.92

Cited by 7 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, micropropagation of banana plantlets is the best choice to produce clones because it provides disease-free planting material with homogeneity of the clones from a certain time regardless of the climate or season. This helps in farm management and increases banana fruit yield [10].…”

Section: Introductionmentioning

confidence: 99%

Effect of biosynthesised silver nanoparticles as sterilant on physiological and biochemical characteristics in micropropagation of Musa sapientum L.

Phongtongpasuk,

Liamnimit,

Buakaew

et al. 2024

Adv. Nat. Sci: Nanosci. Nanotechnol.

View full text Add to dashboard Cite

Green synthesis of silver nanoparticles (AgNPs) using fruit peel extract has gained considerable interest, as it is an eco-friendly and cost-effective method. However, studies on the use of biosynthesised AgNPs to sterilise plant tissue culture medium as an alternative to autoclaving are limited. Thus, this study presented a biogenic method for synthesising AgNPs using mangosteen peel extract. The biosynthesised AgNPs were characterised by different spectroscopic and microscopic methods, including UV-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and energy dispersive x-ray spectroscopy. The results showed that the phytochemical constituents in the mangosteen peel extract (MPE) helped to reduce Ag+ from AgNO3 to metallic silver (Ag0) and stabilise the particles. The formation of biogenic AgNPs was monitored by UV-vis demonstrating a characteristic peak at 425 nm. The AgNPs were spherical and crystalline. The size distribution of the biogenic AgNPs was 5–47 nm with an average diameter of 23.1 ± 6.8 nm. To evaluate the potential of the AgNPs for use as an alternative method to sterilise the culture medium, the antimicrobial activity of the biosynthesised AgNPs (0.1, 1, 10, 100, and 1000 mg l−1) was tested in the banana culture medium. The most feasible concentration of AgNPs to decontaminate the culture medium was 100 mg l−1. Moreover, the results demonstrated that adding 100 mg l−1 AgNPs to the culture medium promoted the growth of the plantlets without any toxic effects. Thus, biogenic AgNPs are a potential biocide to sterilise in vitro banana culture medium.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of biosynthesised silver nanoparticles as sterilant on physiological and biochemical characteristics in micropropagation of Musa sapientum L.

Phongtongpasuk,

Liamnimit,

Buakaew

et al. 2024

Adv. Nat. Sci: Nanosci. Nanotechnol.

View full text Add to dashboard Cite

show abstract

“…Many studies have reported the approaches used in managing lethal browning and contamination in the tissue culture of bananas and plantains [17,[19][20][21][22][23][24]. In general, the browning treatment was carried out by removing phenolic compounds, modifying the redox potential, inhibiting the activation of phenol oxidase enzymes, and reducing phenolase activity and substrate availability by immersing the explants in anti-browning solutions, incorporating anti-browning compounds into the medium, and manipulating the cultural practices [9,17,21].…”

Section: Introductionmentioning

confidence: 99%

“…Meanwhile, the treatment of microbial contamination is generally carried out using the methods of chemotherapy, thermotherapy, and cryotherapy. In addition, chemical agents such as antiseptics, disinfectants, and nanoparticles are also incorporated into plant tissue culture media to control microbial contaminants [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Managing Lethal Browning and Microbial Contamination in Musa spp. Tissue Culture: Synthesis and Perspectives

et al. 2023

View full text Add to dashboard Cite

Browning and contamination are regarded as the main constraints in the plant tissue culture of Musa spp. that can hinder the success of plant propagation in vitro. Browning is caused by enzymatic reactions due to explant injury, while microbial contamination is caused by phyllospheric, rhizospheric, and endophytic microorganisms that reside on, in, and inside the plants. When not properly addressed, they can cause decreased regenerative ability, decreased callus growth, inhibited adventitious shoot growth, and even tissue death. To overcome the browning problem, various attempts have been made in vitro, e.g., immersing the explants in an anti-browning solution, incorporating anti-browning compounds into the medium, and manipulating cultural practices. Correspondingly, to control the problem of contamination, efforts have been made, for example, using various methods, such as thermotherapy, chemotherapy, and cryotherapy, and chemical agents, such as disinfectants, antiseptics, and nanoparticles. This review aims to investigate and provide a comprehensive understanding of the causes of browning and contamination as well as the many approaches used to control browning and contamination problems in Musa spp. tissue cultures.

show abstract

“…However, microbial contamination is a significant obstacle to tissue culture applications and is frequently attributed to ineffective explant sterilization or insufficient sterile equipment (Kim et al, 2017). Banana micropropagation also faces a similar issue, although the majority of the worldwide banana industry relies on tissue culture techniques to generate uniform plants that enable efficient farm management, increased banana fruit yield, and better systems to control pests and diseases (Hamill & Rames, 2018). The majority of the bacteria contamination in banana tissue culture is caused by Brenneria sp., Erwinia sp., Klebsiella sp., Lactococcus sp., Proteus sp., Salmonella sp., Staphylococcus sp.…”

Section: Introductionmentioning

confidence: 99%

Identification and Prevention of Microbial Contaminants in Musa paradisiaca Tissue Culture

Hassen

Badaluddin

Mustapha

et al. 2022

MAB

View full text Add to dashboard Cite

Banana (Musa spp.) is an economically vital fruit crop in Malaysia and therefore, micropropagation was applied to meet the market demand for disease-free seedlings. However, microbial contamination is a significant obstacle in micropropagation techniques. In this research, the microorganisms that were present in the banana culture were characterized and the efficacy of antimicrobial and antifungal agents to inhibit contaminants was evaluated. Three bacterial and two fungal isolates were isolated from the contaminated culture. Klebsiella pneumoniae, Klebsiella quasipneumoniae, and Klebsiella variicola were identified by molecular identification based on the 16S rDNA sequence. The gram-staining method confirmed all three bacteria were gram-negative. Oxidase and catalase tests showed the presence of cytochrome oxidase system and catalase enzyme in all bacteria. The bacteria can also hydrolyze starch, ferment sugars, and reduce sulfur from the amylase test. Phenotypic identification of fungi revealed the presence of conidia and hyphae, indicating the presence of Colletotrichum spp. and Aspergillus spp. In fungi characterization, Colletotrichum gloeosporioides and Aspergillus flavus were detected. Chloramphenicol was identified as an effective antibacterial agent from the disc diffusion method. Fluconazole was a potent antifungal agent by screening the sterilizing agents. The findings may potentially lead the way for the implementation of reducing the contamination rate in banana micropropagation.

show abstract

An effective indexing method for banana tissue culture provides long-term freedom from bacterial contamination

Cited by 7 publications

References 12 publications

Effect of biosynthesised silver nanoparticles as sterilant on physiological and biochemical characteristics in micropropagation of Musa sapientum L.

Effect of biosynthesised silver nanoparticles as sterilant on physiological and biochemical characteristics in micropropagation of Musa sapientum L.

Managing Lethal Browning and Microbial Contamination in Musa spp. Tissue Culture: Synthesis and Perspectives

Identification and Prevention of Microbial Contaminants in Musa paradisiaca Tissue Culture

Contact Info

Product

Resources

About